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The map label for this gene is iucD

Identifier: 218701709

GI number: 218701709

Start: 3573587

End: 3574864

Strand: Direct

Name: iucD

Synonym: ECIAI39_3426

Alternate gene names: 218701709

Gene position: 3573587-3574864 (Clockwise)

Preceding gene: 218701708

Following gene: 218701710

Centisome position: 69.63

GC content: 47.18

Gene sequence:

>1278_bases
ATGAAAAAAAGTGTCGATTTTATTGGTGTAGGGACAGGGCCATTTAATCTCAGCATTGCTGCGTTGTCACATCAGATCGA
AGAACTGGACTGTCTCTTCTTTGATGAACATCCTCATTTTTCCTGGCATCCGGGTATGCTGGTACCGGATTGTCATATGC
AGACCGTCTTTCTGAAAGATCTGGTCAGTGCTGTTGCACCTACAAATCCCTACAGTTTTGTTAACTATCTGGTGAAGCAC
AAAAAGTTCTATCGCTTCCTTACAAGCAGACTACGTACAGTATCCCGTGAAGAGTTTTCTGACTACCTCCGCTGGGCTGC
TGAAGATATGAATAACCTGTATTTCAGTCATACCGTTGAAAACATTGATTTCGATAAAAAACGTCGATTGTTTCTGGTGC
AAACCAGCCAGGGAGAATATTTTGCCCGCAATATCTGCCTTGGTACAGGAAAACAACCTTATTTACCACCCTGTGTGAAG
CATATGACACAATCCTGTTTCCATGCCAGTGAAATGAATCTTCGTCGGCCGGACCTTAGTGGAAAACGGATAACCGTGGT
TGGTGGAGGACAGAGTGGTGCAGACCTGTTCCTTAATGCATTACGCGGGGAATGGGGAGAAGCGGCGGAAATAAACTGGG
TCTCCCGGCGTAATAATTTTAACGCACTGGATGAGGCTGCTTTTGCTGATGAGTATTTTACACCTGAATATATTTCAGGC
TTCTCCGGACTGGAGGAAGATATTCGCCATCAGTTACTGGATGAGCAGAAAATGACATCGGATGGCATCACTGCCGATTC
TTTACTGACCATTTATCGTGAGTTGTACCACCGTTTTGAAGTTCTGAGAAAACCAAGAAATATCCGTCTGCTACCCAGCC
GCTCGGTAACAACTCTGGAAAGTAGTGGTCCTGGCTGGAAGTTGCTGATGGAGCATCATCTGGATCAGGGCAGGGAGAGC
CTGGAAAGTGATGTGGTGATTTTCGCCACAGGTTACCGTTCTGCGTTGCCACAAATACTTCCCTCACTGATGCCCCTGAT
CACCATGCACGATAAGAACACCTTTAAAGTGCGTGATGACTTCACTCTGGAATGGAGTGGCCCGAAAGAGAACAACATCT
TCGTGGTCAACGCCAGTATGCAAACCCATGGCATCGCCGAACCCCAGCTCAGCCTGATGGCATGGAGATCTGCACGTATT
CTTAATCGCGTAATGGGACGTGATTTATTCGATCTCAGTATGCCGCCCGCCCTGATTCAGTGGCGCAGCGGCACCTAG

Upstream 100 bases:

>100_bases
AACTGACCTGGCCTGATCTGGATGGCGGCAGCCGCATGCTGCCGAATTACCTTGAGGATCTGCAAAATCCGCTGTGGCTG
GTAACTCAGGAATATGAATC

Downstream 100 bases:

>100_bases
GGAAAACGCAGCCGGAGGCTGCTTCTTTAACTCGCTACACAGCATCTTTGGGCTGATTTTTTCCGCCCGTATGGAGGAAT
AATGATGATAAGCAAAAAGT

Product: L-lysine 6-monooxygenase

Products: NA

Alternate protein names: Lysine 6-N-hydroxylase; Lysine N(6)-hydroxylase; Lysine-N-oxygenase

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHPGMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKH
KKFYRFLTSRLRTVSREEFSDYLRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRNNFNALDEAAFADEYFTPEYISG
FSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELYHRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRES
LESDVVIFATGYRSALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSLMAWRSARI
LNRVMGRDLFDLSMPPALIQWRSGT

Sequences:

>Translated_425_residues
MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHPGMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKH
KKFYRFLTSRLRTVSREEFSDYLRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRNNFNALDEAAFADEYFTPEYISG
FSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELYHRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRES
LESDVVIFATGYRSALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSLMAWRSARI
LNRVMGRDLFDLSMPPALIQWRSGT
>Mature_425_residues
MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHPGMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKH
KKFYRFLTSRLRTVSREEFSDYLRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRNNFNALDEAAFADEYFTPEYISG
FSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELYHRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRES
LESDVVIFATGYRSALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSLMAWRSARI
LNRVMGRDLFDLSMPPALIQWRSGT

Specific function: This is one of the proteins involved in the biosynthesis of siderophores (such as the dihydroxamate siderophore, aerobactin), which are chelators that mediate the high-affinity iron transport systems induced by the organism under iron-stressed conditions

COG id: COG3486

COG function: function code Q; Lysine/ornithine N-monooxygenase

Gene ontology:

Cell location: Cytoplasm. Cell membrane

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): IUCD_ECOLX (P11295)

Other databases:

- EMBL:   M18968
- EMBL:   U90207
- EMBL:   AF016586
- EMBL:   AF016587
- PIR:   A28665
- RefSeq:   YP_002527487.1
- RefSeq:   YP_002539373.1
- ProteinModelPortal:   P11295
- GeneID:   7324509
- GeneID:   7379560
- ProtClustDB:   CLSK888708
- BioCyc:   MetaCyc:MONOMER-11590
- BRENDA:   1.14.13.59
- GO:   GO:0005737
- InterPro:   IPR013027

Pfam domain/function: PF07992 Pyr_redox_2

EC number: =1.14.13.59

Molecular weight: Translated: 48726; Mature: 48726

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHPGMLVPDCHMQTVFLKD
CCCCCCEEEECCCCCEEEHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
LVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDYLRWAAEDMNNLYFSHTVE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEHHHHHC
NIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVKHMTQSCFHASEMNLRRPDLS
CCCCCCCCEEEEEEECCCCEEEHEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
GKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRNNFNALDEAAFADEYFTPEYISG
CCEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHH
FSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELYHRFEVLRKPRNIRLLPSRSVTTLE
HCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEC
SSGPGWKLLMEHHLDQGRESLESDVVIFATGYRSALPQILPSLMPLITMHDKNTFKVRDD
CCCCCHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHEEECCCCEEEEECC
FTLEWSGPKENNIFVVNASMQTHGIAEPQLSLMAWRSARILNRVMGRDLFDLSMPPALIQ
EEEEECCCCCCCEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEE
WRSGT
ECCCC
>Mature Secondary Structure
MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHPGMLVPDCHMQTVFLKD
CCCCCCEEEECCCCCEEEHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHH
LVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDYLRWAAEDMNNLYFSHTVE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEHHHHHC
NIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVKHMTQSCFHASEMNLRRPDLS
CCCCCCCCEEEEEEECCCCEEEHEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
GKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRNNFNALDEAAFADEYFTPEYISG
CCEEEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHH
FSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELYHRFEVLRKPRNIRLLPSRSVTTLE
HCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEC
SSGPGWKLLMEHHLDQGRESLESDVVIFATGYRSALPQILPSLMPLITMHDKNTFKVRDD
CCCCCHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHEEECCCCEEEEECC
FTLEWSGPKENNIFVVNASMQTHGIAEPQLSLMAWRSARILNRVMGRDLFDLSMPPALIQ
EEEEECCCCCCCEEEEECCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEE
WRSGT
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3275632; 10064136