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The map label for this gene is ygfM

Identifier: 218701589

GI number: 218701589

Start: 3447646

End: 3448425

Strand: Direct

Name: ygfM

Synonym: ECIAI39_3295

Alternate gene names: 218701589

Gene position: 3447646-3448425 (Clockwise)

Preceding gene: 218701588

Following gene: 218701590

Centisome position: 67.18

GC content: 53.85

Gene sequence:

>780_bases
ATGATTGAACAATTTTTCAGACCCGACTCTGTCGAACAAGCGCTGGAACTGAAGCGCCGCTACCAGGATGAAGCCGTCTG
GTTCGCCGGGGGCAGCAAACTCAACGCTACACCAACCCGTACCGATAAAAAGATTGCCATTTCCTTGCAGGATCTGGAGC
TGGACTGGATTGACTGGGATAACGGTGCACTACGAATTGGCGCAATGTCTCGCTTGCAGTCACTGCGTGATGCACGATTT
ATTCCTGCAGCACTGCGTGAAGCTCTCGGTTTTGTTTACTCACGCCATGTTCGTAATCAGTCGACCATTGGTGGTGAAAT
CGCCGCCCGGCAGGAAGAGTCGGTGCTGCTTCCCGTCCTGCTGGCACTGGATGCTGAACTGGTTTTTGGCAACGGCGAAA
CGCTGTCAATCGAGGACTACCTGGCCTGCCCATGCGATCGCCTGTTAACCGAAATTATCATTAAAGATCCGTATCGCACC
TGTGCGACCCGCAAAATTAGCCGTTCTCAGGCAGGTTTAACCGTCGTGACGGCAGCCGTTGCAATGACAGACCACGACGG
TATGCGAATTGCGCTGGATGGCGTCGCCAGTAAAGCACTGCGTCTGCACGATGTTGAAAAACAAAACCTGGAAGGCAATG
CACTTGAACAGGCTGTCGCCAACGCCATTTTCCCGCAGGAAGATTTGCGGGGCAGCGTGGCCTATAAACGCTATATCACG
GGAGTTCTGGTAGCCGACCTGTATGCCGACTGCCAACAGGCTGGGGAGGAAGCCGTATGA

Upstream 100 bases:

>100_bases
CCAGAAAAGCCGCTGCCAGTATGTGGCGTCGGATGGATGCGCTGGCATAAATAACAGCTCCCCTCTTCTTTCAGAGAAGA
GGGGAAATTAAGGAAAGACG

Downstream 100 bases:

>100_bases
TCATCCACTTTACTTTAAATGGCGCGCCTCAGGAGCTAACCGTTAATCCAGGCGAAAACGTGCAAAAGCTGTTGTTTAAC
ATGGGAATGCACTCTGTACG

Product: putative selenate reductase subunit YgfM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKKIAISLQDLELDWIDWDNGALRIGAMSRLQSLRDARF
IPAALREALGFVYSRHVRNQSTIGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQAVANAIFPQEDLRGSVAYKRYIT
GVLVADLYADCQQAGEEAV

Sequences:

>Translated_259_residues
MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKKIAISLQDLELDWIDWDNGALRIGAMSRLQSLRDARF
IPAALREALGFVYSRHVRNQSTIGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQAVANAIFPQEDLRGSVAYKRYIT
GVLVADLYADCQQAGEEAV
>Mature_259_residues
MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKKIAISLQDLELDWIDWDNGALRIGAMSRLQSLRDARF
IPAALREALGFVYSRHVRNQSTIGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQAVANAIFPQEDLRGSVAYKRYIT
GVLVADLYADCQQAGEEAV

Specific function: Unknown

COG id: COG1319

COG function: function code C; Aerobic-type carbon monoxide dehydrogenase, middle subunit CoxM/CutM homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Escherichia coli, GI1789245, Length=259, Percent_Identity=99.2277992277992, Blast_Score=526, Evalue=1e-151,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005107
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR002346
- InterPro:   IPR017698 [H]

Pfam domain/function: PF03450 CO_deh_flav_C; PF00941 FAD_binding_5 [H]

EC number: NA

Molecular weight: Translated: 28654; Mature: 28654

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKKIAISLQDLELDWIDWD
CCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEEEEEEECC
NGALRIGAMSRLQSLRDARFIPAALREALGFVYSRHVRNQSTIGGEIAARQEESVLLPVL
CCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHH
LALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRTCATRKISRSQAGLTVVTAAV
HHHCCEEEECCCCEEEEHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHH
AMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQAVANAIFPQEDLRGSVAYKRYIT
HEECCCCCEEEECHHHHHHHHHHHCHHCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHH
GVLVADLYADCQQAGEEAV
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKKIAISLQDLELDWIDWD
CCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEEEEEEEEEEECC
NGALRIGAMSRLQSLRDARFIPAALREALGFVYSRHVRNQSTIGGEIAARQEESVLLPVL
CCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCHHHHHH
LALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRTCATRKISRSQAGLTVVTAAV
HHHCCEEEECCCCEEEEHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHH
AMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQAVANAIFPQEDLRGSVAYKRYIT
HEECCCCCEEEECHHHHHHHHHHHCHHCCCCCHHHHHHHHHHCCCHHHHCCHHHHHHHHH
GVLVADLYADCQQAGEEAV
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]