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The map label for this gene is queF

Identifier: 218701511

GI number: 218701511

Start: 3353939

End: 3354787

Strand: Direct

Name: queF

Synonym: ECIAI39_3216

Alternate gene names: 218701511

Gene position: 3353939-3354787 (Clockwise)

Preceding gene: 218701492

Following gene: 218701512

Centisome position: 65.35

GC content: 49.12

Gene sequence:

>849_bases
ATGTCTTCTTATGCAAACCATCAGGCACTTGCGGGCCTGACTCTTGGAAAATCAACCGATTACCGGGATACCTATGACGC
CAGCCTACTGCAAGGCGTTCCGCGTAGTCTGAATCGCGATCCGCTGGGTCTGAAAGCGGATAACCTGCCTTTTCACGGTA
CGGATATCTGGACGCTATATGAACTTTCCTGGCTGAATGCGAAAGGTTTGCCGCAGGTCGCCGTCGGTCATGTTGAACTT
GATTACACCAGCGCAAATCTGATTGAGTCGAAGAGTTTTAAGCTCTATCTCAACAGTTTTAACCAGACGCGTTTTAATAA
CTGGGATGAGGTGCGCCAGACGCTGGAGCGCGACTTAAGCACTTGCGCTCAGGGTAAGGTTAGCGTGGCGTTATATCGTC
TTGATGAACTGGAAGGCCAACCGATAGGTCATTTCAATGGCACTTGCATTGATGACCAGGATATCACTATCGATAACTAT
GAATTCACTACTGACTATCTGGAGAATGCCACCAGTGGTGAAAAAGTGGTGGAAGAGACGCTTGTCAGCCACCTGCTGAA
ATCAAACTGCCTGATCACCCATCAACCAGATTGGGGATCGATCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAA
AACTGCTGCGTTACCTGGTCTCTTTCCGCCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTG
TTACGCTTCTGCCAGCCAGAGAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCG
CAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAA

Upstream 100 bases:

>100_bases
ATCTTTCAGGGCTTGTGCGGTCAAATCGTCCACTAATTTCTCTCTTCACTTAAACCAGATACACTTGTCGTTTAGTTTAT
CTGGTTTATGACGGTGAAAC

Downstream 100 bases:

>100_bases
ATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGA
ATGACGATATTCGTCCCATA

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGLKADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVEL
DYTSANLIESKSFKLYLNSFNQTRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRYLVSFRHHNEFHEQCVERIFNDL
LRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPSTTRLVRQ

Sequences:

>Translated_282_residues
MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGLKADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVEL
DYTSANLIESKSFKLYLNSFNQTRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRYLVSFRHHNEFHEQCVERIFNDL
LRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPSTTRLVRQ
>Mature_281_residues
SSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGLKADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELD
YTSANLIESKSFKLYLNSFNQTRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNYE
FTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRYLVSFRHHNEFHEQCVERIFNDLL
RFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPSTTRLVRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=282, Percent_Identity=98.5815602836879, Blast_Score=582, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 32530; Mature: 32398

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGLKADNLPFHGTDIWTLY
CCCCCCCHHHHEEEECCCCCCCCHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCEEEEE
ELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQTRFNNWDEVRQTLERDLS
EEEECCCCCCCCEEEEEEEEECCCHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHH
TCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNYEFTTDYLENATSGEKVVEET
HHHCCCEEEEEEEHHHCCCCCCCCCCCCEECCCCCEECCCCCHHHHHHCCCCHHHHHHHH
LVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRYLVSFRHHNEFHEQCVERIFNDL
HHHHHHHCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPSTTRLVRQ
HHHCCCCHHEEEEEHHHCCCCCCCCCCCCCCCCCCHHHHHCC
>Mature Secondary Structure 
SSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGLKADNLPFHGTDIWTLY
CCCCCCHHHHEEEECCCCCCCCHHHHHHHHHCHHHCCCCCCCCCCCCCCCCCCCEEEEE
ELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQTRFNNWDEVRQTLERDLS
EEEECCCCCCCCEEEEEEEEECCCHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHH
TCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNYEFTTDYLENATSGEKVVEET
HHHCCCEEEEEEEHHHCCCCCCCCCCCCEECCCCCEECCCCCHHHHHHCCCCHHHHHHHH
LVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRYLVSFRHHNEFHEQCVERIFNDL
HHHHHHHCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPSTTRLVRQ
HHHCCCCHHEEEEEHHHCCCCCCCCCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA