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The map label for this gene is yihU

Identifier: 218701418

GI number: 218701418

Start: 3245531

End: 3246427

Strand: Direct

Name: yihU

Synonym: ECIAI39_3118

Alternate gene names: 218701418

Gene position: 3245531-3246427 (Clockwise)

Preceding gene: 218701408

Following gene: 218701419

Centisome position: 63.24

GC content: 55.96

Gene sequence:

>897_bases
ATGGCAGCAATCGCGTTTATCGGTTTAGGACAAATGGGTTCGCCAATGGCGAGCAATTTATTGCAGCAAGGGCATCAACT
TTGCGTCTTCGATGTGAACGCCGAAGCTGTGCGGCATCTGGTAGACAAAGGTGCGACTCCCGCCGCCAACCCGGCGCAGG
CCGCTAAAGATGCCGAATTTATCATTACCATGTTGCCGAATGGCGATCTGGTGCGCAGCGTGTTGTTCGGTGAAAACGGC
GTTTGCGAAAGCTTATCTACCGATGCGCTGGTCATTGATATGTCGACCATCCATCCGCTGCAAACCGATAAATTGATTGC
CGATATGCAGGCCAGAGGCTTCAGCATGATGGATGTTCCGGTAGGCCGTACTTCTGCAAATGCCATTACCGGTACTCTGT
TACTGCTGGCTGGCGGCACCGCTGAACAAGTTGAACGCGCCACACCGATCCTGATGGCGATGGGCAGCGAGTTGATCAAC
GCGGGCGGTCCGGGCATGGGGATCCGCGTTAAGCTTATCAACAACTACATGAGCATCGCGCTCAATGCGTTGTCGGCAGA
AGCCGCCGTTTTGTGCGAAGCCCTCAATCTTCCCTTCGATGTTGCCGTCAAGGTAATGAGCGGTACTGCCGCCGGTAAAG
GCCACTTCACCACTTCCTGGCCGAACAAAGTCCTCAGCGGGGATCTTTCTCCCGCCTTCATGATCGATCTTGCCCATAAG
GATCTTGGCATCGCCCTTGATGTCGCCAACCAGTTGCATGTGCCGATGCCACTGGGGGCCGCCTCACGGGAGGTTTATAG
CCAGGCGCGCGCAGCGGGTCGCGGTCGCCAGGACTGGTCCGCCATTCTGGAGCAGGTCCGTGTCAGTGCCGGGATGACTG
CCAAAGTAAAAATGTAA

Upstream 100 bases:

>100_bases
GTCAATTTTTTGACGATAAATTACAAATACGATCAAAAACAGACAAGCAAGGCATGTCATAAATGACAAAAAGTGACATC
ACTGTATTCAGGAGAGGGTT

Downstream 100 bases:

>100_bases
CGACTGGATAAAGGAATAAATGAATGAATAAGTACACCATCAACGACATTACGCGTGCATCGGGCGGTTTTGCCATGCTG
GCGGTCGATCAGCGCGAAGC

Product: putative oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MAAIAFIGLGQMGSPMASNLLQQGHQLCVFDVNAEAVRHLVDKGATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENG
VCESLSTDALVIDMSTIHPLQTDKLIADMQARGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHK
DLGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILEQVRVSAGMTAKVKM

Sequences:

>Translated_298_residues
MAAIAFIGLGQMGSPMASNLLQQGHQLCVFDVNAEAVRHLVDKGATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENG
VCESLSTDALVIDMSTIHPLQTDKLIADMQARGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHK
DLGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILEQVRVSAGMTAKVKM
>Mature_297_residues
AAIAFIGLGQMGSPMASNLLQQGHQLCVFDVNAEAVRHLVDKGATPAANPAQAAKDAEFIITMLPNGDLVRSVLFGENGV
CESLSTDALVIDMSTIHPLQTDKLIADMQARGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELINA
GGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHKD
LGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILEQVRVSAGMTAKVKM

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=31.6151202749141, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI40556376, Length=281, Percent_Identity=25.6227758007117, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1790315, Length=298, Percent_Identity=98.6577181208054, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI145693186, Length=280, Percent_Identity=36.0714285714286, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1786719, Length=289, Percent_Identity=31.1418685121107, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1789092, Length=292, Percent_Identity=29.7945205479452, Blast_Score=116, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17557316, Length=283, Percent_Identity=29.6819787985866, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24655230, Length=292, Percent_Identity=32.8767123287671, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI19922568, Length=292, Percent_Identity=32.8767123287671, Blast_Score=140, Evalue=8e-34,
Organism=Drosophila melanogaster, GI28574115, Length=287, Percent_Identity=22.9965156794425, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24655240, Length=64, Percent_Identity=53.125, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 31137; Mature: 31005

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIAFIGLGQMGSPMASNLLQQGHQLCVFDVNAEAVRHLVDKGATPAANPAQAAKDAEF
CCEEEEEECCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCCCHHHHCCCCEE
IITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQTDKLIADMQARGFSMMDVP
EEEECCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEECC
VGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELINAGGPGMGIRVKLINNYMSIA
CCCCCCHHHHEEEEEEECCCHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHH
LNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHK
HHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCCCCEECCCCHHHHCCCCCCEEEEEECCC
DLGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILEQVRVSAGMTAKVKM
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure 
AAIAFIGLGQMGSPMASNLLQQGHQLCVFDVNAEAVRHLVDKGATPAANPAQAAKDAEF
CEEEEEECCCCCCHHHHHHHHCCCCEEEEECCHHHHHHHHHCCCCCCCCHHHHCCCCEE
IITMLPNGDLVRSVLFGENGVCESLSTDALVIDMSTIHPLQTDKLIADMQARGFSMMDVP
EEEECCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEECC
VGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELINAGGPGMGIRVKLINNYMSIA
CCCCCCHHHHEEEEEEECCCHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHH
LNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHK
HHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCCCCEECCCCHHHHCCCCCCEEEEEECCC
DLGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILEQVRVSAGMTAKVKM
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8346018; 9278503 [H]