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The map label for this gene is frwB

Identifier: 218701340

GI number: 218701340

Start: 3161650

End: 3161970

Strand: Reverse

Name: frwB

Synonym: ECIAI39_3039

Alternate gene names: 218701340

Gene position: 3161970-3161650 (Counterclockwise)

Preceding gene: 218701341

Following gene: 218701339

Centisome position: 61.61

GC content: 50.78

Gene sequence:

>321_bases
ATGACGAAAATTATTGCAGTAACCGCGTGTCCATCTGGTGTTGCCCATACTTATATGGCGGCAGAGGCGCTGGAAAGTGC
CGCGAAAGCGAAAGGCTGGGACGTGAAAGTAGAAACCCAGGGGTCGATTGGTCTGGAAAATGAACTGACTGCGGAAGATG
TGGCGAGCGCCGATATGGTGATTCTGACCAAAGATATCGGCATCAAGTTTGAAGAGCGTTTTGCTGGCAAGACCATCGTG
CGCGTCAACATCAGCGACGCGGTAAAACGCGCTGATGCCATTATGAGCAAAATTGAAGCTCACCTGGCACAAACCGCTTA
A

Upstream 100 bases:

>100_bases
ACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACTTGGATTTGGATTTTGAAATT
AATTAATTGAGGAACGAGCC

Downstream 100 bases:

>100_bases
GTTCTTATCCCGCCCGTAAGGGCGGGTTCGCTTTCCCACAGGAGTTCCTCATGACGAATCGTATCTCTCGCCTCAAAACT
GCACTGTTTGCCAATACCCG

Product: putative PTS system fructose-like transporter subunit EIIB

Products: NA

Alternate protein names: PTS system fructose-like EIIB component 2 [H]

Number of amino acids: Translated: 106; Mature: 105

Protein sequence:

>106_residues
MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWDVKVETQGSIGLENELTAEDVASADMVILTKDIGIKFEERFAGKTIV
RVNISDAVKRADAIMSKIEAHLAQTA

Sequences:

>Translated_106_residues
MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWDVKVETQGSIGLENELTAEDVASADMVILTKDIGIKFEERFAGKTIV
RVNISDAVKRADAIMSKIEAHLAQTA
>Mature_105_residues
TKIIAVTACPSGVAHTYMAAEALESAAKAKGWDVKVETQGSIGLENELTAEDVASADMVILTKDIGIKFEERFAGKTIVR
VNISDAVKRADAIMSKIEAHLAQTA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1445

COG function: function code G; Phosphotransferase system fructose-specific component IIB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790387, Length=106, Percent_Identity=99.0566037735849, Blast_Score=210, Evalue=2e-56,
Organism=Escherichia coli, GI1788492, Length=88, Percent_Identity=53.4090909090909, Blast_Score=100, Evalue=3e-23,
Organism=Escherichia coli, GI87082348, Length=98, Percent_Identity=50, Blast_Score=99, Evalue=6e-23,
Organism=Escherichia coli, GI1788730, Length=101, Percent_Identity=44.5544554455446, Blast_Score=96, Evalue=4e-22,
Organism=Escherichia coli, GI1790390, Length=98, Percent_Identity=43.8775510204082, Blast_Score=87, Evalue=3e-19,
Organism=Escherichia coli, GI1786951, Length=94, Percent_Identity=43.6170212765958, Blast_Score=86, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003353 [H]

Pfam domain/function: PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 11234; Mature: 11103

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS51099 PTS_EIIB_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWDVKVETQGSIGLENELTAEDVASADMV
CCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHCCCCEE
ILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHLAQTA
EEEECCCCEEHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKIIAVTACPSGVAHTYMAAEALESAAKAKGWDVKVETQGSIGLENELTAEDVASADMV
CEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHCCCCEE
ILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHLAQTA
EEEECCCCEEHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]