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The map label for this gene is murI

Identifier: 218701323

GI number: 218701323

Start: 3139670

End: 3140527

Strand: Reverse

Name: murI

Synonym: ECIAI39_3022

Alternate gene names: 218701323

Gene position: 3140527-3139670 (Counterclockwise)

Preceding gene: 218701324

Following gene: 218701321

Centisome position: 61.19

GC content: 51.63

Gene sequence:

>858_bases
ATGGCTACCAAACTGCAGGACGGGAATACACCTTGTCTGGCAGCTACACCTTCTGAACCACGTCCCACCGTGCTGGTGTT
TGACTCCGGCGTCGGTGGGTTGTCGGTCTATGACGAGATCCGGCATCTCTTACCGGATCTCCATTACATTTATGCTTTCG
ATAACGTCGCTTTCCCGTATGGCGAAAAAAGCGAAGCGTTTATTGTTGAGCGAGTGGTGGCAATTGTCACCGCAGTGCAA
AAACGTTATCCCCTTGCGCTGGCTGTGGTCGCTTGCAACACTGCCAGTACCGTTTCACTTCCTGCATTACGCGAAAAGTT
CGACTTCCCGGTTGTTGGTGTCGTGCCGGCGATTAAACCTGCTGCACGTCTGACGGCAAATGGCATTGTCGGATTACTGG
CAACCCGCGGAACAGTTAAACGTTCTTATACTCATGAGCTGATCGCGCGTTTCGCTAATGAATGCCAGATAGAAATGCTG
GGCTCGGCAGAGATGGTTGAGTTGGCTGAAGCGAAGCTGCATGGCGAAGATGTTTCTCTGGATGCGCTAAAACGTATCTT
GCGTCCGTGGTTAAGAATGAAAGAGCCGCCAGATACCGTTGTACTGGGGTGTACCCATTTCCCTCTACTACAAGAAGAAC
TGTTACAGGTGCTGCCAGAGGGAACCCGGCTGGTGGATTCTGGCGCAGCGATTGCTCGCCGAACGGCCTGGCTGTTAGAA
CATGAAGCCCCGGATGCAAAATCTGCCGATGCGAATATTGCCTTTTGTATGGCAATGACGCCAGAAGCTGAACAATTATT
GCCCGTTTTACAGCGTTACGGCTTCGAAACGCTCGAAAAACTGGCAGTTTTAGGCTGA

Upstream 100 bases:

>100_bases
GTGAGCTTGTGGGATCTTGCGGTTGCGTATCCGGTCACCTCTCATCTGACAGTTCGTGGTAAAATAGCCAACCTGTTCGA
CAAAGATTATGAGACAGTCT

Downstream 100 bases:

>100_bases
TTTGGTTGAATGTTGCGCGGTCAGAAAATTATTTTAAATTTCCTCTTGTCAGGCCGGAATAACTCCCTATAATGCGCCAC
CACTGACACGGAACAACGGC

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLLPDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQ
KRYPLALAVVACNTASTVSLPALREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVLPEGTRLVDSGAAIARRTAWLLE
HEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFETLEKLAVLG

Sequences:

>Translated_285_residues
MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLLPDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQ
KRYPLALAVVACNTASTVSLPALREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVLPEGTRLVDSGAAIARRTAWLLE
HEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFETLEKLAVLG
>Mature_284_residues
ATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLLPDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQK
RYPLALAVVACNTASTVSLPALREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEMLG
SAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVLPEGTRLVDSGAAIARRTAWLLEH
EAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFETLEKLAVLG

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=285, Percent_Identity=99.2982456140351, Blast_Score=575, Evalue=1e-165,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 31073; Mature: 30942

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00923 ASP_GLU_RACEMASE_1 ; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLLPDLHYIYAFDNVAFPY
CCCCCCCCCCCEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCEECCC
GEKSEAFIVERVVAIVTAVQKRYPLALAVVACNTASTVSLPALREKFDFPVVGVVPAIKP
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHCCCCEEECCCCCCH
AARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEMLGSAEMVELAEAKLHGEDVSL
HHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCH
DALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVLPEGTRLVDSGAAIARRTAWLLE
HHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHH
HEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFETLEKLAVLG
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
ATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLLPDLHYIYAFDNVAFPY
CCCCCCCCCCEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEEECCEECCC
GEKSEAFIVERVVAIVTAVQKRYPLALAVVACNTASTVSLPALREKFDFPVVGVVPAIKP
CCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHCCCCEEECCCCCCH
AARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEMLGSAEMVELAEAKLHGEDVSL
HHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCH
DALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVLPEGTRLVDSGAAIARRTAWLLE
HHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHH
HEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFETLEKLAVLG
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA