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The map label for this gene is wecC

Identifier: 218701299

GI number: 218701299

Start: 3108685

End: 3109947

Strand: Reverse

Name: wecC

Synonym: ECIAI39_3000

Alternate gene names: 218701299

Gene position: 3109947-3108685 (Counterclockwise)

Preceding gene: 218701300

Following gene: 218701298

Centisome position: 60.6

GC content: 53.6

Gene sequence:

>1263_bases
ATGAGTTTTGCGACCATTTCTGTTATCGGACTGGGTTACATCGGGCTGCCTACAGCAGCTGCGTTTGCCTCAAGGCAAAA
ACAGGTGATTGGTGTCGATATCAACCAACACGCGGTTGATACCATCAATCGTGGCGAAATCCATATCGTCGAACCTGATT
TGGCGAGCGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTG
ATTGCTGTACCCACGCCGTTTAAAGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCC
AGTGCTGAAAAAAGGCGCGCTGGTGATCCTTGAATCCACCTCGCCGGTGGGGTCAACCGAGAAGATGGCAGAATGGTTAG
CAGAGATGCGTCCAGATCTCACTTTCCCACAGCAGGTGGGCGAGTTGGCGGACGTCAACATTGCTTACTGTCCGGAACGC
GTGTTACCGGGCCAGGTTATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCCCG
CGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCA
CCGAAAACAGCTTCCGCGATGTGAATATCGCTTTTGCTAACGAACTGTCGCTGATTTGTGCCGATCAGGGAATTAACGTC
TGGGAACTGATTCGCCTGGCGAATCGTCACCCTCGCGTTAATATTCTTCAGCCTGGTCCTGGCGTGGGCGGTCACTGCAT
TGCTGTTGATCCGTGGTTTATCGTGGCACAGAACCCGCAGCAGGCGCGGCTTATCCGTACCGCGCGCGAAGTGAACGATC
ACAAACCGTTCTGGGTTATCGATCAGGTGAAAGCGGCGGTTGCCGACTGCCTTGCTGCTACCGATAAACGCGCCAGTGAA
CTGAAAATCGCCTGCTTTGGTCTGGCGTTTAAACCGAATATTGATGACCTGCGCGAAAGCCCGGCGATGGAAATCGCTGA
ACTGATCGCCCAGTGGCATAGCGGCGAAACGCTGGTTGTTGAGCCTAACATCCACCAGTTGCCGAAAAAACTGACCGGGC
TTTGTACTCTGGCGCAGCTTGACGAGGCGCTGGCAACGGCAGACGTGCTGGTGATGCTGGTCGATCATAGTCAGTTCAAA
GTTATCAATGGCGACAATGTCCATCAGCAGTATGTCGTCGATGCCAAAGGAGTTTGGCGCTGA

Upstream 100 bases:

>100_bases
AAAACGAATATCAAGCTATGAGCCGCGCCCATAACCCGTATGGTGATGGTCAGGCATGCTCTCGCATTCTGGAAGCGTTA
AAAAATAATCGGATATCACT

Downstream 100 bases:

>100_bases
TGAGAAAGATTCTGATAACAGGTGGTGCCGGGTTTATTGGCTCGGCGCTGGTGCGTTATATCATCAACGAAACGAGCGAC
GCGGTGGTAGTGGTCGATAA

Product: UDP-N-acetyl-D-mannosamine dehydrogenase

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: UDP-ManNAc 6-dehydrogenase [H]

Number of amino acids: Translated: 420; Mature: 419

Protein sequence:

>420_residues
MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINRGEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWL
IAVPTPFKGDHEPDMTYVESAARSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSFRDVNIAFANELSLICADQGINV
WELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVAQNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASE
LKIACFGLAFKPNIDDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVMLVDHSQFK
VINGDNVHQQYVVDAKGVWR

Sequences:

>Translated_420_residues
MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINRGEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWL
IAVPTPFKGDHEPDMTYVESAARSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSFRDVNIAFANELSLICADQGINV
WELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVAQNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASE
LKIACFGLAFKPNIDDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVMLVDHSQFK
VINGDNVHQQYVVDAKGVWR
>Mature_419_residues
SFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINRGEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLI
AVPTPFKGDHEPDMTYVESAARSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPERV
LPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSFRDVNIAFANELSLICADQGINVW
ELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVAQNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASEL
KIACFGLAFKPNIDDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVMLVDHSQFKV
INGDNVHQQYVVDAKGVWR

Specific function: Catalyzes the four-electron oxidation of UDP-N-acetyl-D- mannosamine (UDP-ManNAc), reducing NAD(+) and releasing UDP-N- acetylmannosaminuronic acid (UDP-ManNAcA) [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4507813, Length=457, Percent_Identity=26.6958424507659, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI296040438, Length=358, Percent_Identity=25.1396648044693, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI48994968, Length=420, Percent_Identity=99.7619047619048, Blast_Score=862, Evalue=0.0,
Organism=Escherichia coli, GI1788340, Length=376, Percent_Identity=23.6702127659574, Blast_Score=69, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17560350, Length=437, Percent_Identity=28.8329519450801, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17136908, Length=370, Percent_Identity=27.5675675675676, Blast_Score=107, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 45824; Mature: 45693

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINRGEIHIVEPDLASVVKT
CCEEEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCHHHCCCCCCEEEEECCHHHHHHHH
AVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAARSIAPVLKKGALVILEST
HHCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECC
SPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPERVLPGQVMVELIKNDRVIGGM
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCCCEECCC
TPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSFRDVNIAFANELSLICADQGINV
CCHHHHHHHHEEEEEECCEEEEECCCHHHHHHHHCCCCCEEEEEEECCEEEEEECCCCCH
WELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVAQNPQQARLIRTAREVNDHKPFWVI
HHHHHHHCCCCCEEEECCCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHHCCCCCEEEH
DQVKAAVADCLAATDKRASELKIACFGLAFKPNIDDLRESPAMEIAELIAQWHSGETLVV
HHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHHHCCHHHHHHHHHHHHCCCCEEEE
EPNIHQLPKKLTGLCTLAQLDEALATADVLVMLVDHSQFKVINGDNVHQQYVVDAKGVWR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
SFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINRGEIHIVEPDLASVVKT
CEEEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCHHHCCCCCCEEEEECCHHHHHHHH
AVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAARSIAPVLKKGALVILEST
HHCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECC
SPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPERVLPGQVMVELIKNDRVIGGM
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCCCEECCC
TPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSFRDVNIAFANELSLICADQGINV
CCHHHHHHHHEEEEEECCEEEEECCCHHHHHHHHCCCCCEEEEEEECCEEEEEECCCCCH
WELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVAQNPQQARLIRTAREVNDHKPFWVI
HHHHHHHCCCCCEEEECCCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHHCCCCCEEEH
DQVKAAVADCLAATDKRASELKIACFGLAFKPNIDDLRESPAMEIAELIAQWHSGETLVV
HHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHHHCCHHHHHHHHHHHHCCCCEEEE
EPNIHQLPKKLTGLCTLAQLDEALATADVLVMLVDHSQFKVINGDNVHQQYVVDAKGVWR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEECCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]