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The map label for this gene is iap

Identifier: 218701244

GI number: 218701244

Start: 3045713

End: 3046750

Strand: Direct

Name: iap

Synonym: ECIAI39_2942

Alternate gene names: 218701244

Gene position: 3045713-3046750 (Clockwise)

Preceding gene: 218701231

Following gene: 218701249

Centisome position: 59.35

GC content: 49.52

Gene sequence:

>1038_bases
ATGTTTTCCGCATTGCGCCACCGTACCGCTGCCCTGGCGCTCGGCGTATGCTTTATCCTCCCTGTCCAGGCTGCCACTGT
AAGACCTGGAGATATTGCCAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCAGCAG
AAATGTTATCTGCCGATTATATTCGCCAACAGTTTCAGCAAATGGGTTATCGCAGTGATATTCGGACATTTAATAGTCGG
TATATTTATACCGCCCGCGATAATCGCAAGAGCTGGCATAACGTGACGGGAAGTACGGTGATTGCCGCTCATGAAGGCAA
AGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCG
GCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGGTTTAGGTGTCATGCTGGAATTGGCAGAACGCCTGAAAAAT
ACGCCTACCGAGTATGGTATTCGATTTGTGGCGACCAGCGGCGAAGAGGAAGGGAAATTAGGCGCTGAGAATTTACTCAA
GCGGATGAGTGACTCCGAAAAGAAAAATACACTGCTGGTGATTAATCTCGATAACTTAATTGTTGGCGATAAATTGTATT
TCAACAGCGGTGTAAAAACCCCTGAAGCAGTAAGGAAATTAACGCGCGACAGGGCGCTGGCAATTGCGCGTAGTCATGGA
ATTGCCGCAACGACCAATCCGGGTTTGAATAAAAATTATCCGAAGGGTACTGGCTGTTGTAATGATGCCGAAGTTTTCGA
CAAAGCCGGTATTGCGGTACTTTCGGTGGAGGCGACCAACTGGAATCTTGGAAATAAGGATGGTTATCAGCAACGCTCAA
AAACACCGGCCTTCCCGGCGGGAAATAGCTGGCATGATGTAAGGCTTGATAATCAACAGCATATTGATAAGGCGCTTCCT
GGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCGCTGGTGAAGGAGCTGGCGAAGGCGTCTTGA

Upstream 100 bases:

>100_bases
CCGGATTTCGGGCGTTTAGGAAGATTTGAAATTGTTTTAGCGCAGCGGCAGTTTCATACTATGGCGGTAAAAAAATTTGC
ATGGTATTTAAGGACTCACT

Downstream 100 bases:

>100_bases
TGGGTTTGAAATGATTACTCCCTTGCCTGCAGGAAATCAATGCCACCAGAAACAGAGTATAAAAAAGGGGTGCAGGAGGC
CAAACTTTAACAATTCAATA

Product: alkaline phosphatase isozyme conversion aminopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MFSALRHRTAALALGVCFILPVQAATVRPGDIANTQARHIATFFPGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSR
YIYTARDNRKSWHNVTGSTVIAAHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
TPTEYGIRFVATSGEEEGKLGAENLLKRMSDSEKKNTLLVINLDNLIVGDKLYFNSGVKTPEAVRKLTRDRALAIARSHG
IAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSVEATNWNLGNKDGYQQRSKTPAFPAGNSWHDVRLDNQQHIDKALP
GRIERRCRDVMRIMLPLVKELAKAS

Sequences:

>Translated_345_residues
MFSALRHRTAALALGVCFILPVQAATVRPGDIANTQARHIATFFPGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSR
YIYTARDNRKSWHNVTGSTVIAAHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
TPTEYGIRFVATSGEEEGKLGAENLLKRMSDSEKKNTLLVINLDNLIVGDKLYFNSGVKTPEAVRKLTRDRALAIARSHG
IAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSVEATNWNLGNKDGYQQRSKTPAFPAGNSWHDVRLDNQQHIDKALP
GRIERRCRDVMRIMLPLVKELAKAS
>Mature_345_residues
MFSALRHRTAALALGVCFILPVQAATVRPGDIANTQARHIATFFPGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSR
YIYTARDNRKSWHNVTGSTVIAAHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
TPTEYGIRFVATSGEEEGKLGAENLLKRMSDSEKKNTLLVINLDNLIVGDKLYFNSGVKTPEAVRKLTRDRALAIARSHG
IAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSVEATNWNLGNKDGYQQRSKTPAFPAGNSWHDVRLDNQQHIDKALP
GRIERRCRDVMRIMLPLVKELAKAS

Specific function: This protein, presumably an aminopeptidase, mediates the conversion of E.coli alkaline phosphatase isozyme 1, to isozymes 2 and 3 by removing, one by one, the two amino-terminal arginine residues [H]

COG id: COG2234

COG function: function code R; Predicted aminopeptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M28 family. M28C subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789111, Length=345, Percent_Identity=97.1014492753623, Blast_Score=703, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007484 [H]

Pfam domain/function: PF04389 Peptidase_M28 [H]

EC number: 3.4.11.- [C]

Molecular weight: Translated: 37885; Mature: 37885

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSALRHRTAALALGVCFILPVQAATVRPGDIANTQARHIATFFPGRMTGTPAEMLSADY
CCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHEECCCCCCCCHHHHHHHHH
IRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIAAHEGKAPQQIIIMAHLDT
HHHHHHHCCCHHHHHHCCCEEEEEECCCCCHHHCCCCCEEEEECCCCCCCEEEEEEECCC
YAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKNTPTEYGIRFVATSGEEEGKL
CCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHCEEEEEECCCCCCCC
GAENLLKRMSDSEKKNTLLVINLDNLIVGDKLYFNSGVKTPEAVRKLTRDRALAIARSHG
CHHHHHHHHCCCCCCCEEEEEEECCEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
IAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSVEATNWNLGNKDGYQQRSKTPAFPA
CEEECCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCCCCCHHHHCCCCCCCC
GNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIMLPLVKELAKAS
CCCCCEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFSALRHRTAALALGVCFILPVQAATVRPGDIANTQARHIATFFPGRMTGTPAEMLSADY
CCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHEECCCCCCCCHHHHHHHHH
IRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIAAHEGKAPQQIIIMAHLDT
HHHHHHHCCCHHHHHHCCCEEEEEECCCCCHHHCCCCCEEEEECCCCCCCEEEEEEECCC
YAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKNTPTEYGIRFVATSGEEEGKL
CCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHCEEEEEECCCCCCCC
GAENLLKRMSDSEKKNTLLVINLDNLIVGDKLYFNSGVKTPEAVRKLTRDRALAIARSHG
CHHHHHHHHCCCCCCCEEEEEEECCEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
IAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSVEATNWNLGNKDGYQQRSKTPAFPA
CEEECCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEEECCCCCCCCCCHHHHCCCCCCCC
GNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIMLPLVKELAKAS
CCCCCEEEECCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Aminopeptidases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316184; 9278503; 1316900 [H]