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The map label for this gene is pphB

Identifier: 218701227

GI number: 218701227

Start: 3032051

End: 3032707

Strand: Direct

Name: pphB

Synonym: ECIAI39_2924

Alternate gene names: 218701227

Gene position: 3032051-3032707 (Clockwise)

Preceding gene: 218701226

Following gene: 218701231

Centisome position: 59.08

GC content: 42.77

Gene sequence:

>657_bases
ATGCCATCTACACGCTATCAAAAAATCAATGCCCATCACTATCGCCATATATGGGCCGTTGGTGATATTCATGGTGACTA
TCAATTATTACAATCCCGCTTACATCAACTCTCTTTTTGCCCTGAAACCGACTTACTTATTTCTGTCGGCGATAATATTG
ATCGTGGGCCGGAGAGTCTTAACGTGCTGCGCCTGCTAAACCAGCCCTGGTTTACATCGGTTAAAGGCAACCACGAAGCG
ATGGCGTTGGATGCATTCGAAACTGGCGATGGAAATATGTGGCTTGCCAGCGGTGGAGACTGGTTTTTCGATTTAAATGA
TTCAGAGCTACAAGCGGCAATAGATCTGTTGCTGAAATTCCATCACCTTCCACATATTATTGAAGTTACTAACGACAACA
TAAAATATGTCATCGCACATGCGGATTATCCAGGTGATGAATATCAGTTTGGCAAAGAAATAGCAGAAAGCGAATTACTC
TGGCCTGTTGATCGCGTACTGAAATCGCTCAAAGGCGAGTTACAGGAAATAAACGGCGCCGACTGCTTTATCTTCGGTCA
TATGATGTTTGATAACATCCAGACATTTGCCAATCAGGTTTATATTGATACCGGCTCGCCGGAAAGCAGACGGTTATCGT
TTTATAAAATTAAATAA

Upstream 100 bases:

>100_bases
ATTCCCGATAGCCTTTTGCTATCGGGAATATTAACGACAACTGACGAATCAAATAAAAATACCCTGTATAATAGGAAATC
TTATTTTACAGGGTAAAACC

Downstream 100 bases:

>100_bases
CGTGGCCGGGTAAAGCAAAGCCTCTACCCGGCACTTTTTTATTAACACTTACCTTCCGCCAGCAGCGGCGGGATGCTCGG
CACCATCGGCGCGTCATCAA

Product: serine/threonine-specific protein phosphatase 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MPSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETDLLISVGDNIDRGPESLNVLRLLNQPWFTSVKGNHEA
MALDAFETGDGNMWLASGGDWFFDLNDSELQAAIDLLLKFHHLPHIIEVTNDNIKYVIAHADYPGDEYQFGKEIAESELL
WPVDRVLKSLKGELQEINGADCFIFGHMMFDNIQTFANQVYIDTGSPESRRLSFYKIK

Sequences:

>Translated_218_residues
MPSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETDLLISVGDNIDRGPESLNVLRLLNQPWFTSVKGNHEA
MALDAFETGDGNMWLASGGDWFFDLNDSELQAAIDLLLKFHHLPHIIEVTNDNIKYVIAHADYPGDEYQFGKEIAESELL
WPVDRVLKSLKGELQEINGADCFIFGHMMFDNIQTFANQVYIDTGSPESRRLSFYKIK
>Mature_217_residues
PSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETDLLISVGDNIDRGPESLNVLRLLNQPWFTSVKGNHEAM
ALDAFETGDGNMWLASGGDWFFDLNDSELQAAIDLLLKFHHLPHIIEVTNDNIKYVIAHADYPGDEYQFGKEIAESELLW
PVDRVLKSLKGELQEINGADCFIFGHMMFDNIQTFANQVYIDTGSPESRRLSFYKIK

Specific function: Has been shown, in vitro, to act on Ser, Thr and Tyr- phosphorylated substrates [H]

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPP phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1789090, Length=218, Percent_Identity=90.8256880733945, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI87081988, Length=217, Percent_Identity=45.1612903225806, Blast_Score=187, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 25010; Mature: 24879

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00125 SER_THR_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETDLLISVGDNIDRGPESL
CCCCCCEECCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHH
NVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWFFDLNDSELQAAIDLLLKF
HHHHHHCCCCEECCCCCCCEEEEEEEECCCCCEEEECCCCEEEECCCHHHHHHHHHHHHH
HHLPHIIEVTNDNIKYVIAHADYPGDEYQFGKEIAESELLWPVDRVLKSLKGELQEINGA
HCCCEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
DCFIFGHMMFDNIQTFANQVYIDTGSPESRRLSFYKIK
EEEEEHHHHHHHHHHHHHEEEEECCCCCCCEEEEEEEC
>Mature Secondary Structure 
PSTRYQKINAHHYRHIWAVGDIHGDYQLLQSRLHQLSFCPETDLLISVGDNIDRGPESL
CCCCCEECCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHH
NVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWFFDLNDSELQAAIDLLLKF
HHHHHHCCCCEECCCCCCCEEEEEEEECCCCCEEEECCCCEEEECCCHHHHHHHHHHHHH
HHLPHIIEVTNDNIKYVIAHADYPGDEYQFGKEIAESELLWPVDRVLKSLKGELQEINGA
HCCCEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
DCFIFGHMMFDNIQTFANQVYIDTGSPESRRLSFYKIK
EEEEEHHHHHHHHHHHHHEEEEECCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9130712; 9278503 [H]