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The map label for this gene is hycD

Identifier: 218701214

GI number: 218701214

Start: 3018314

End: 3019237

Strand: Reverse

Name: hycD

Synonym: ECIAI39_2910

Alternate gene names: 218701214

Gene position: 3019237-3018314 (Counterclockwise)

Preceding gene: 218701215

Following gene: 218701213

Centisome position: 58.83

GC content: 57.9

Gene sequence:

>924_bases
ATGAGTGTTTTATATCCGTTAATTCAGGCGCTGGTGTTATTTGCCGTTGCGCCGCTGCTCTCCGGTATTACCCGCGTAGC
GCGCGCCCGCTTGCATAACCGTCGTGGGCCGGGCGTGTTGCAGGAGTATCGCGACATTATCAAACTGCTGGGGCGTCAGA
GCGTTGGCCCGGATGCCTCCGGCTGGGTGTTCCGCCTGACGCCGTATGTGATGGTGGGCGTCATGCTGACTATCGCCACT
GCGCTGCCGGTGGTGACCGTCGGTTCTCCGCTGCCGCAACTGGGCGATTTGATCACCTTACTGTATCTCTTCGCCATCGC
GCGTTTCTTCTTTGCCATTTCTGGTCTGGATACCGGTAGCCCGTTTACCGCTATCGGCGCGAGCCGTGAAGCGATGCTTG
GCGTGTTGGTTGAACCGATGCTGCTGCTTGGTCTGTGGGTCGCCGCACAGGTTGCCGGTTCCACCAACATCAGCAACATC
ACCGACACCGTTTATCACTGGCCACTGAGCCAGAGCATCCCGCTGGTGCTGGCGCTTTGTGCCTGTGCGTTCGCCACCTT
TATCGAAATGGGCAAACTGCCGTTCGACCTGGCGGAAGCCGAGCAGGAGTTGCAGGAAGGTCCGCTCTCCGAATACAGCG
GTAGCGGCTTTGGCGTCATGAAATGGGGTATCAGCCTGAAACAGCTGGTGGTGTTGCAGATGTTCGTCGGGGTGTTTATT
CCGTGGGGACAAATGGAAACCTTCACCGCTGGTGGACTGCTGCTGGCGCTGGTGATTGCCATCATCAAACTGGTGGTTGG
CGTCCTGGTTATCGCGCTGTTCGAAAACAGCATGGCCCGTCTGCGTCTTGATATTACTCCGCGCATTACCTGGGCTGGGT
TTGGCTTTGCATTTTTAGCGTTCGTCTCCTTGCTGGCGGCGTGA

Upstream 100 bases:

>100_bases
CGGGCTGGCAGTGCGAGGGGAGTGCGTTGCTGTTCCGCCGGATGGCGCTGGTTGAACTGGCGGTACTGGTGGTGATTATT
GTTTCACGAGGAGCCTGAGA

Downstream 100 bases:

>100_bases
TTAAAGAGAGTTTGAGCATGTCTGAAGAAAAATTAGGTCAACATTATCTCGCCGCGCTGAATGAGGCATTTCCGGGCGTC
GTGCTGGACCACGCGTGGCA

Product: hydrogenase 3, membrane subunit

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIAT
ALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
PWGQMETFTAGGLLLALVIAIIKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLAFVSLLAA

Sequences:

>Translated_307_residues
MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIAT
ALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
PWGQMETFTAGGLLLALVIAIIKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLAFVSLLAA
>Mature_306_residues
SVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATA
LPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNIT
DTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFIP
WGQMETFTAGGLLLALVIAIIKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLAFVSLLAA

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=307, Percent_Identity=99.6742671009772, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI87082115, Length=303, Percent_Identity=52.1452145214521, Blast_Score=284, Evalue=4e-78,
Organism=Escherichia coli, GI1788618, Length=306, Percent_Identity=25.8169934640523, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 33044; Mature: 32913

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00667 COMPLEX1_ND1_1 ; PS00668 COMPLEX1_ND1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSVGPDAS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC
GWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPLSQSIPLVLALC
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHH
ACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PWGQMETFTAGGLLLALVIAIIKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCEEHHHHHHHHHH
FVSLLAA
HHHHHCC
>Mature Secondary Structure 
SVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSVGPDAS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCC
GWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPLSQSIPLVLALC
CCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHH
ACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PWGQMETFTAGGLLLALVIAIIKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCEEHHHHHHHHHH
FVSLLAA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]