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The map label for this gene is ascB

Identifier: 218701206

GI number: 218701206

Start: 3011380

End: 3012804

Strand: Direct

Name: ascB

Synonym: ECIAI39_2902

Alternate gene names: 218701206

Gene position: 3011380-3012804 (Clockwise)

Preceding gene: 218701205

Following gene: 218701218

Centisome position: 58.68

GC content: 51.72

Gene sequence:

>1425_bases
ATGTCAGTATTTCCGCAAGGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTACCGTGAAGGTGG
CAAAGGGCTGACCACCGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGACTGGAAAAACGTTTTC
AGTTGCGTGATGACGAGTTTTATCCCAGCCATGAAGCGACGGATTTTTATCATCGTTATAAAGAAGATATCGCCCTGATG
GCAGAGATGGGGTTCAAAGTGTTCCGTACCTCAATTGCCTGGAGCCGTCTCTTTCCGCAGGGCGATGAACTGACGCCCAA
CCAGCAGGGCATTGCTTTTTACCGTTCTGTCTTTGAAGAGTGTAAAAAGTACGGTATCGAACCGCTGGTCACGTTGTGCC
ACTTCGATGTGCCGATGCATCTGGTCACCGAATATGGCTCCTGGCGTAACCGCAAGCTGGTGGAGTTTTTCAGCCGCTAC
GCCAGAACCTGCTTTGAAGCATTTGATGGTCTGGTGAAATACTGGCTTACCTTCAATGAAATCAACATTATGTTGCATAG
CCCGTTCTCCGGCGCGGGTCTGGTGTTTGAAGAAGGGGAAAATCAGGATCAGGTGAAATATCAGGCTGCGCACCACCAGC
TGATTGCCAGTGCGTTAGCCACCAAAATCGCCCATGAAGTTAACCCGCAAAATCAGGTGGGCTGTATGCTGGCGGGCGGT
AACTTCTACCCTTACAGTTGCAAGCCGGAAGATGTCTGGGCGGCGCTGGAGAAAGATCGGGAAAACCTGTTTTTTATCGA
TGTGCAGGCGCGGGGCGCGTATCCGGCTTACTCTGCCCGCGTATTCCGCGAAAAAGGGGTAACCATCGACAAAGCACCGG
GCGATGATGAGATCCTGAAAAACACCGTCGATTTTGTCTCTTTCAGCTATTACGCCTCGCGCTGCGCCTCGGCGGAGATG
AACGCCAACAATAGCAGTGCGGCGAACGTAGTGAAATCGCTGCGTAACCCGTATCTGCAAGTGAGCGACTGGGGCTGGGG
TATTGATCCACTCGGTCTGCGTATCACCATGAATATGATGTACGACCGTTATCAGAAGCCGCTGTTTCTGGTGGAAAACG
GCCTCGGCGCAAAAGATGAATTTGCTGCCAACGGTGAGATTAACGATGACTATCGCATCAGCTATTTACGCGAACATATC
CGCGCAATGGGCGAAGCGATTGCAGATGGCATTCCGCTGATGGGCTACACCACATGGGGCTGTATTGATTTAGTTTCCGC
CTCAACGGGTGAAATGAGCAAACGCTACGGTTTTGTCTATGTAGACCGTGACGATGCAGGCAACGGCACGCTGGCGCGCA
CGCGTAAGAAATCGTTCTGGTGGTATAAAAAAGTAATTGCCAGTAATGGTGAGGATTTAGAGTAA

Upstream 100 bases:

>100_bases
TTGAGGATATTCCTGTTGAGGAAGCGACTGCCGAGGCGCGAAAGCATCAGAGCGCACAACCGACCGTCGCCAAAGAAGTA
AGTCTTAATTGAGGATGAAA

Downstream 100 bases:

>100_bases
GTAACCGTGCCGGATGCAGCGTGATGAACTGAACCCCAAAACTTGGACAGTTTCAGTTAACCGGCTTTTTGATACTCAGT
CCGGTATTCTACCGGGCTGA

Product: cryptic 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSVFPQGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALM
AEMGFKVFRTSIAWSRLFPQGDELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASALATKIAHEVNPQNQVGCMLAGG
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEM
NANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYRISYLREHI
RAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGTLARTRKKSFWWYKKVIASNGEDLE

Sequences:

>Translated_474_residues
MSVFPQGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALM
AEMGFKVFRTSIAWSRLFPQGDELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASALATKIAHEVNPQNQVGCMLAGG
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEM
NANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYRISYLREHI
RAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGTLARTRKKSFWWYKKVIASNGEDLE
>Mature_473_residues
SVFPQGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMA
EMGFKVFRTSIAWSRLFPQGDELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRYA
RTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASALATKIAHEVNPQNQVGCMLAGGN
FYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMN
ANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDEFAANGEINDDYRISYLREHIR
AMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGTLARTRKKSFWWYKKVIASNGEDLE

Specific function: Can hydrolyze salicin, cellobiose, and probably arbutin [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=499, Percent_Identity=30.4609218436874, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI32481206, Length=494, Percent_Identity=28.7449392712551, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI13273313, Length=487, Percent_Identity=27.7207392197125, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI24497614, Length=490, Percent_Identity=27.1428571428571, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI28376633, Length=491, Percent_Identity=24.2362525458248, Blast_Score=129, Evalue=7e-30,
Organism=Escherichia coli, GI1789070, Length=474, Percent_Identity=97.8902953586498, Blast_Score=980, Evalue=0.0,
Organism=Escherichia coli, GI2367270, Length=474, Percent_Identity=54.0084388185654, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI2367174, Length=478, Percent_Identity=51.4644351464435, Blast_Score=506, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17552856, Length=487, Percent_Identity=29.9794661190965, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17539390, Length=495, Percent_Identity=30.3030303030303, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI21356577, Length=473, Percent_Identity=24.9471458773784, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 53833; Mature: 53702

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVFPQGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEF
CCCCCCCCEECCEEECCCCCCCHHCCCCCCEEEEECCCCCCEEEEEECCHHHCCCCCCCC
YPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGDELTPNQQGIAFYRSVFEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
CKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRYARTCFEAFDGLVKYWLTFNE
HHHCCCCHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
INIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASALATKIAHEVNPQNQVGCMLAGG
EEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKAPGDDEILK
CCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEECCCCCHHHHH
NTVDFVSFSYYASRCASAEMNANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMM
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEHHHH
YDRYQKPLFLVENGLGAKDEFAANGEINDDYRISYLREHIRAMGEAIADGIPLMGYTTWG
HHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
CIDLVSASTGEMSKRYGFVYVDRDDAGNGTLARTRKKSFWWYKKVIASNGEDLE
HHHHHCCCCCCHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SVFPQGFLWGGALAANQSEGAYREGGKGLTTVDMIPHGEHRMAVKLGLEKRFQLRDDEF
CCCCCCCEECCEEECCCCCCCHHCCCCCCEEEEECCCCCCEEEEEECCHHHCCCCCCCC
YPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGDELTPNQQGIAFYRSVFEE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
CKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRYARTCFEAFDGLVKYWLTFNE
HHHCCCCHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
INIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLIASALATKIAHEVNPQNQVGCMLAGG
EEEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
NFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPAYSARVFREKGVTIDKAPGDDEILK
CCCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEECCCCCHHHHH
NTVDFVSFSYYASRCASAEMNANNSSAANVVKSLRNPYLQVSDWGWGIDPLGLRITMNMM
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEHHHH
YDRYQKPLFLVENGLGAKDEFAANGEINDDYRISYLREHIRAMGEAIADGIPLMGYTTWG
HHHHCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
CIDLVSASTGEMSKRYGFVYVDRDDAGNGTLARTRKKSFWWYKKVIASNGEDLE
HHHHHCCCCCCHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]