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The map label for this gene is norW

Identifier: 218701201

GI number: 218701201

Start: 3004364

End: 3005497

Strand: Direct

Name: norW

Synonym: ECIAI39_2897

Alternate gene names: 218701201

Gene position: 3004364-3005497 (Clockwise)

Preceding gene: 218701200

Following gene: 218701205

Centisome position: 58.54

GC content: 54.06

Gene sequence:

>1134_bases
ATGAGCAACAGCATTGTGATCATTGGTTCGGGCTTCGCCGCCCGCCAACTGGTGAAAAATATTCGCAAACAGGACGCCAG
TATTCCATTAACTCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGC
AACGTGCCGACGACCTTACCCGCCAGACGGCAGGTGAATTTGCCGAGCAGTTTAATCTGCGCCTGTTTCCGCACACCTGG
GTAACGGATATCGATGCCGAAGCCCATGTGGTGAAAAGTCAGAATAATCAGTGGCAATACGACAAGTTAGTGCTGGCAAC
CGGTGCCAGCGCCTTTGTCCCGCCAGTGCCCGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCT
GTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGCTTGATTGGTATCGAACTGGCGATGGATTTT
TGTCGTGCAGGCAAAGCGGTCACGCTAATCGACAACGCTGCCAGTATTCTGGCATCGTTAATGCTACCGGAAGTAAGCAG
CCGCTTGCAGCATCGGTTGACGGAGATGGGCGTTCATCTGCTGCTAAAATCTCAGTTGCAGGGGCTGGAAAAAACGGATT
CTGGCATTCTGGCAACGCTGGACCGCCAGCGCAGCATCGAAGTGGATGCGGTAATTGCCGCCACCGGGCTGCGCCCGGAA
ACCGCCCTGGCACGACGCGCCGGGCTGACGATTAATCGTGGCGTTTGCGTCGATAGTTATCTGCAAACCAGTAATGCCGA
TATTTATGCGCTGGGCGATTGCGCGGAAATTAACGGTCAGGTATTGCCGTTCCTCCAGCCGATTCAACTTAGCGCAATGG
TGCTGGCAAAAAATCTTCTCGGCAATAACACGCCGCTGAAACTCCCGGCGATGCTGGTGAAAATCAAAACGCCAGAATTA
CCGCTGCATCTGGCAGGCGAAACCCAGCGTCAGGATTTACGCTGGCAAATTAATACCGAACGCCAGGGAATGGTGGCGCG
CGGTGTTGACGATGGTGACCAGCTTCGCGCCTTTGTGGTCAGTGAGGATCGGATGAAAGAGGCGTTTGGATTGTTGAAAA
CGTTGCCGATGTAG

Upstream 100 bases:

>100_bases
TTGCGCCAGGAACGCCGTGGAGTGAAGTCCCGGATAACTTCCTGTGCCCGGAATGCTCTCTCGGCAAAGACGTCTTTGAC
GAACTGGCATCGGAGGCAAA

Downstream 100 bases:

>100_bases
GCATGGTTTTCTCCCTCGCCCAAAGGGCGAGGGGACTGTCCGCGCTACTTTTAACGCTTCGTTACGCCACATCCGCCACT
ATGCAGCATGCAGGATGCGC

Product: nitric oxide reductase

Products: NAD; reduced flavorubredoxin; H2O [C]

Alternate protein names: Flavorubredoxin reductase; FlRd-reductase; FlavoRb reductase [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSNSIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKPDLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTW
VTDIDAEAHVVKSQNNQWQYDKLVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGIELAMDF
CRAGKAVTLIDNAASILASLMLPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILATLDRQRSIEVDAVIAATGLRPE
TALARRAGLTINRGVCVDSYLQTSNADIYALGDCAEINGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPEL
PLHLAGETQRQDLRWQINTERQGMVARGVDDGDQLRAFVVSEDRMKEAFGLLKTLPM

Sequences:

>Translated_377_residues
MSNSIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKPDLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTW
VTDIDAEAHVVKSQNNQWQYDKLVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGIELAMDF
CRAGKAVTLIDNAASILASLMLPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILATLDRQRSIEVDAVIAATGLRPE
TALARRAGLTINRGVCVDSYLQTSNADIYALGDCAEINGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPEL
PLHLAGETQRQDLRWQINTERQGMVARGVDDGDQLRAFVVSEDRMKEAFGLLKTLPM
>Mature_376_residues
SNSIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKPDLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWV
TDIDAEAHVVKSQNNQWQYDKLVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGIELAMDFC
RAGKAVTLIDNAASILASLMLPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILATLDRQRSIEVDAVIAATGLRPET
ALARRAGLTINRGVCVDSYLQTSNADIYALGDCAEINGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELP
LHLAGETQRQDLRWQINTERQGMVARGVDDGDQLRAFVVSEDRMKEAFGLLKTLPM

Specific function: One of at least two accessory proteins for anaerobic nitric oxide (NO) reductase. Reduces the rubredoxin moiety of NO reductase [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=315, Percent_Identity=21.9047619047619, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI226437568, Length=315, Percent_Identity=21.9047619047619, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI65787454, Length=315, Percent_Identity=21.9047619047619, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789065, Length=377, Percent_Identity=97.0822281167109, Blast_Score=746, Evalue=0.0,
Organism=Escherichia coli, GI1789765, Length=323, Percent_Identity=25.6965944272446, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1788892, Length=275, Percent_Identity=26.5454545454545, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787352, Length=246, Percent_Identity=29.2682926829268, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17559934, Length=276, Percent_Identity=25.3623188405797, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24639257, Length=373, Percent_Identity=22.7882037533512, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281359715, Length=373, Percent_Identity=22.7882037533512, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281359713, Length=373, Percent_Identity=22.7882037533512, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24639250, Length=373, Percent_Identity=22.7882037533512, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI18543267, Length=373, Percent_Identity=22.7882037533512, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24639252, Length=373, Percent_Identity=22.7882037533512, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24585130, Length=280, Percent_Identity=21.7857142857143, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 41385; Mature: 41254

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKPDLSHVISQGQRADDLT
CCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCHHHHH
RQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDKLVLATGASAFVPPVPGRE
HHHHHHHHHHCCCEECCCCCEECCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCE
LMLTLNSQQEYRACETQLRDARRVLIVGGGLIGIELAMDFCRAGKAVTLIDNAASILASL
EEEEECCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHH
MLPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILATLDRQRSIEVDAVIAATGLRPE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEEEECCCCCH
TALARRAGLTINRGVCVDSYLQTSNADIYALGDCAEINGQVLPFLQPIQLSAMVLAKNLL
HHHHHHCCCEEECCCCHHHHHHCCCCCEEEECCHHHCCCCCCCCCCHHHHHHHHHHHHHH
GNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLRWQINTERQGMVARGVDDGDQLRAFVV
CCCCCCEECEEEEEEECCCCCEEECCCCCCCCCEEEECCCCCCCEEECCCCHHHEEEEEE
SEDRMKEAFGLLKTLPM
CHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNSIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKPDLSHVISQGQRADDLT
CCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCHHHHH
RQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDKLVLATGASAFVPPVPGRE
HHHHHHHHHHCCCEECCCCCEECCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCCCE
LMLTLNSQQEYRACETQLRDARRVLIVGGGLIGIELAMDFCRAGKAVTLIDNAASILASL
EEEEECCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEHHHHHHHHHH
MLPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILATLDRQRSIEVDAVIAATGLRPE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEEEECCCCCH
TALARRAGLTINRGVCVDSYLQTSNADIYALGDCAEINGQVLPFLQPIQLSAMVLAKNLL
HHHHHHCCCEEECCCCHHHHHHCCCCCEEEECCHHHCCCCCCCCCCHHHHHHHHHHHHHH
GNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLRWQINTERQGMVARGVDDGDQLRAFVV
CCCCCCEECEEEEEEECCCCCEEECCCCCCCCCEEEECCCCCCCEEECCCCHHHEEEEEE
SEDRMKEAFGLLKTLPM
CHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; oxidized flavorubredoxin; O2 [C]

Specific reaction: NADH + oxidized flavorubredoxin + O2 = NAD + reduced flavorubredoxin + H2O [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA