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The map label for this gene is ygaF

Identifier: 218701151

GI number: 218701151

Start: 2956084

End: 2957352

Strand: Direct

Name: ygaF

Synonym: ECIAI39_2846

Alternate gene names: 218701151

Gene position: 2956084-2957352 (Clockwise)

Preceding gene: 218701150

Following gene: 218701152

Centisome position: 57.6

GC content: 57.37

Gene sequence:

>1269_bases
ATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTGATTGATGTTTATCCGGATGC
CCGCATTGCGTTGCTGGAAAAAGAGTCCGGCCCGGCCTTTCATCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGG
TCTATTACACGCCCGGTAGCCTGAAGGCACAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAC
GGCATTCGCTATGACAACTGCGGCAAGATGCTGGTCGCCACCTCTGAACTCGAAATGGAACGGATGCGCGCGTTGTGGGA
ACGCACGGCGGCGAACGGTATCGAGCGCGAGTGGTTAAACGCCGAGGAACTGCGCGAGCGCGAACCGAATATCACCGGGC
TCGGCGGCATTTTTGTGCCGTCCAGCGGCATTGTCAGCTATCGCGAAGTGACGGCGGCGATGGCAAAAATCTTCCAGGCC
AGAGGCGGCGAGATTATTTATAACGCCGAAGTCAGCGCCCTCAGTGAGCATAAAAACGGCGTGGTGATACGTACCCGTCA
GGGCGGTGAATATGAAGCATCGACGCTGATTAGCTGTTCCGGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGCCTCG
AACCGGGCTTTATTATCTGCCCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCATAACCAGATTGTTAACCACCTG
ATTTACCCCATTCCCGACCCCGCGATGCCATTTTTGGGCGTGCATCTCACCCGCATGATCGATGGCAGCGTGACCGTCGG
GCCAAACGCGGTGCTGGCTTTCAAACGTGAAGGCTATCGCAAGCGCGACTTCTCATTTAGCGACACGCTGGAGATTTTGG
GCTCGTCGGGGATTCGCCGGGTGCTGCAAAACCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGC
GGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGC
GCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATG
CGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAG
AGTAACCCCGGACGCACGCTGCGAGCGGCACGTAGTGTGGATGCCTTACACGCCGCATTTAATCAATAA

Upstream 100 bases:

>100_bases
CACCCGGATCTGCGCCGTGAGCTGATGCGTCAGCGTGGCTATTTCGCTTACGCCACTAACCACTACCAGACGCATCAGTA
AGCGCAAAGGAATTGAGCGG

Downstream 100 bases:

>100_bases
CCTTTGAAAACAGGACGTAACAATGAAACTTAACGACAGTAACTTATTCCGCCAGCAGGCGTTAATTAACGGGGAGTGGC
TGGACGCCAACAATGGCGAA

Product: hydroxyglutarate oxidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPAFHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQN
GIRYDNCGKMLVATSELEMERMRALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNQIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKS
GYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQ
SNPGRTLRAARSVDALHAAFNQ

Sequences:

>Translated_422_residues
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPAFHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQN
GIRYDNCGKMLVATSELEMERMRALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNQIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKS
GYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQ
SNPGRTLRAARSVDALHAAFNQ
>Mature_422_residues
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPAFHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQN
GIRYDNCGKMLVATSELEMERMRALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNQIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKS
GYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQ
SNPGRTLRAARSVDALHAAFNQ

Specific function: Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate [H]

COG id: COG0579

COG function: function code R; Predicted dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L2HGDH family [H]

Homologues:

Organism=Homo sapiens, GI13376331, Length=398, Percent_Identity=41.4572864321608, Blast_Score=322, Evalue=6e-88,
Organism=Escherichia coli, GI87082148, Length=422, Percent_Identity=98.1042654028436, Blast_Score=860, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17565764, Length=401, Percent_Identity=42.8927680798005, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI24585081, Length=407, Percent_Identity=39.5577395577396, Blast_Score=280, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015657
- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: =1.1.3.15 [H]

Molecular weight: Translated: 46126; Mature: 46126

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPAFHQTGHNSGVIHAGVYYTPGS
CCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHCCCCCCCEEEEEEEECCCC
LKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERMRALWERTAANGIEREWLN
CEEEEEEECCCHHHHHHCCCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHCCC
AEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQARGGEIIYNAEVSALSEHKNG
HHHHHHCCCCCEECCEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHCCCC
VVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNQIVNHL
EEEEECCCCCCCHHHHEEHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCHHHHHHHC
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRR
CCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHH
VLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEHHE
LFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQSNPGRTLRAARSVDALHAAF
EEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
NQ
CC
>Mature Secondary Structure
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPAFHQTGHNSGVIHAGVYYTPGS
CCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHCCCCCCCEEEEEEEECCCC
LKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERMRALWERTAANGIEREWLN
CEEEEEEECCCHHHHHHCCCCCEECCCCCEEEEEHHHHHHHHHHHHHHHHHCCCHHHCCC
AEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQARGGEIIYNAEVSALSEHKNG
HHHHHHCCCCCEECCEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHCCCC
VVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNQIVNHL
EEEEECCCCCCCHHHHEEHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCHHHHHHHC
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRR
CCCCCCCCCHHHHHHHHHHCCCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHCCHHHHH
VLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEHHE
LFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQSNPGRTLRAARSVDALHAAF
EEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
NQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 8297211; 7984428 [H]