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The map label for this gene is ecoRIIR [H]

Identifier: 218701132

GI number: 218701132

Start: 2933379

End: 2934593

Strand: Direct

Name: ecoRIIR [H]

Synonym: ECIAI39_2824

Alternate gene names: 218701132

Gene position: 2933379-2934593 (Clockwise)

Preceding gene: 218701131

Following gene: 218701134

Centisome position: 57.16

GC content: 41.89

Gene sequence:

>1215_bases
ATGTATATGTCGGTTTTTCATAACTGGCTGCTTGATATCGCAAGCGGGAATTACTTTATCTACATCAAACGCCTTTCTGC
AAACGACACTGGCGCAACAGGTGGCCATCAGGTCGGACTTTATATCCCCTCAAATATCGTTGAAAAGCTTTTTCCATCTA
TCAATCATACTCGCGAACTGAACCCTTCAGTCTTCCTTACTGCGCATGTATCATCCCATGATTGCCCTGATACCCAAGCA
CGCGCAATTTATTACAACAACCGTTATTTTGGTAAGACCCGAAACGAAAAAAGAATTACGCGCTGGGGGAGAGGAAGTCC
ATTACAGAACCCTGAAAATACAGGAGCTCTCACAATTCTTGCTTTCAGGTTAAACGAACAGAACACTGACTGTTCCGAGG
TAGATATATGGGTCTGCGTCAATCCCGATGAAGAGGATATCATCGAGTCTGCTATTGGCGAAATCATACCTGGAACCCTA
ATTTCCGGCCCTGCCGGACAAATTTTGGGCGGATTATCTCTTCAGCAAACTCCAGTAAATCATAAATATGTTATTCCTGA
AGACTGGAAGAAGCGTTTTCCTTCTGGAAACGAAATTATTCAATATGCTGCTGGCCATTATGCTAAAAACTCCAAGGATC
CAGATGAGCAACTGATTGACCGTCGGCGTGTCGAGTATGATATTTTTCTACTCGTCGAGGAATTACATGTTCTTGATATT
ATTAAGAAAGGATTCGATTCTGTAGATGAGTTTATTGCATTAGCCAACTCTGTCAGTAATCGACGTAAATCAAGGGCAGG
CAAATCACTTGAACTTCACCTAGAGAAGCTTTTTATCGAGCACGGACTACGGCATTTCTCCACTCAGGCAGTTACTGAAG
GTAATAAAAAACCAGATTTCCTGTTTCCTTCAGCAGAGGCATATCATAACGTTGAATTTCCTGTAGAAAACTTACGTATG
CTGGCAGTAAAGACCACCTGCAAAGATCGCTGGCGTCAGATACTGAATGAAGCAGATAAAATCCATCAGGTACATTTATT
TACACTGCAAGAAGGTGTTTCTTCAGCACAATACCGAGAAATGAAAGATGCGGGTGTCAGACTCGTTGTACCATCAACTT
TACATAAAAAATACCCAGAAGCAGTTAGAGAAGAATTAATAACGCTCGGAGCATTCATTACTGAGCTGATAGAGCTTTAC
GCTGAACTATCATAG

Upstream 100 bases:

>100_bases
AAAGCCCGCTTATGCGGGCTTTTTGTTTTTCCCTTAAGTTCCTAGCCGCTTATCTATAACTATGGAAAAATGTTAACCCT
GACTGTATGCTAACAAAGGG

Downstream 100 bases:

>100_bases
GCTGACTCCCGGCTTAAAAGGCCGGGAGGTGTTCTCAAGGCTGCCCAGTCTTACCAGCATCAGCAGAAACAGCTTTGAGG
ATATAGGGTTCCAGAAGTCT

Product: Type-2 restriction enzyme EcoRII (R.EcoRII) (Type II restriction enzyme EcoRII) (Endonuclease EcoRII)

Products: NA

Alternate protein names: R.EcoRII; Endonuclease EcoRII; Type II restriction enzyme EcoRII [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MYMSVFHNWLLDIASGNYFIYIKRLSANDTGATGGHQVGLYIPSNIVEKLFPSINHTRELNPSVFLTAHVSSHDCPDTQA
RAIYYNNRYFGKTRNEKRITRWGRGSPLQNPENTGALTILAFRLNEQNTDCSEVDIWVCVNPDEEDIIESAIGEIIPGTL
ISGPAGQILGGLSLQQTPVNHKYVIPEDWKKRFPSGNEIIQYAAGHYAKNSKDPDEQLIDRRRVEYDIFLLVEELHVLDI
IKKGFDSVDEFIALANSVSNRRKSRAGKSLELHLEKLFIEHGLRHFSTQAVTEGNKKPDFLFPSAEAYHNVEFPVENLRM
LAVKTTCKDRWRQILNEADKIHQVHLFTLQEGVSSAQYREMKDAGVRLVVPSTLHKKYPEAVREELITLGAFITELIELY
AELS

Sequences:

>Translated_404_residues
MYMSVFHNWLLDIASGNYFIYIKRLSANDTGATGGHQVGLYIPSNIVEKLFPSINHTRELNPSVFLTAHVSSHDCPDTQA
RAIYYNNRYFGKTRNEKRITRWGRGSPLQNPENTGALTILAFRLNEQNTDCSEVDIWVCVNPDEEDIIESAIGEIIPGTL
ISGPAGQILGGLSLQQTPVNHKYVIPEDWKKRFPSGNEIIQYAAGHYAKNSKDPDEQLIDRRRVEYDIFLLVEELHVLDI
IKKGFDSVDEFIALANSVSNRRKSRAGKSLELHLEKLFIEHGLRHFSTQAVTEGNKKPDFLFPSAEAYHNVEFPVENLRM
LAVKTTCKDRWRQILNEADKIHQVHLFTLQEGVSSAQYREMKDAGVRLVVPSTLHKKYPEAVREELITLGAFITELIELY
AELS
>Mature_404_residues
MYMSVFHNWLLDIASGNYFIYIKRLSANDTGATGGHQVGLYIPSNIVEKLFPSINHTRELNPSVFLTAHVSSHDCPDTQA
RAIYYNNRYFGKTRNEKRITRWGRGSPLQNPENTGALTILAFRLNEQNTDCSEVDIWVCVNPDEEDIIESAIGEIIPGTL
ISGPAGQILGGLSLQQTPVNHKYVIPEDWKKRFPSGNEIIQYAAGHYAKNSKDPDEQLIDRRRVEYDIFLLVEELHVLDI
IKKGFDSVDEFIALANSVSNRRKSRAGKSLELHLEKLFIEHGLRHFSTQAVTEGNKKPDFLFPSAEAYHNVEFPVENLRM
LAVKTTCKDRWRQILNEADKIHQVHLFTLQEGVSSAQYREMKDAGVRLVVPSTLHKKYPEAVREELITLGAFITELIELY
AELS

Specific function: Recognizes the double-stranded sequence CCWGG and cleaves before C-1 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011335
- InterPro:   IPR015109
- InterPro:   IPR015300 [H]

Pfam domain/function: PF09019 EcoRII-C; PF09217 EcoRII-N [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 45938; Mature: 45938

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYMSVFHNWLLDIASGNYFIYIKRLSANDTGATGGHQVGLYIPSNIVEKLFPSINHTREL
CHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCEEEEEECHHHHHHHCCCCCCCCCC
NPSVFLTAHVSSHDCPDTQARAIYYNNRYFGKTRNEKRITRWGRGSPLQNPENTGALTIL
CCCEEEEEECCCCCCCCCCCEEEEECCEEECCCCCCHHHHHCCCCCCCCCCCCCCEEEEE
AFRLNEQNTDCSEVDIWVCVNPDEEDIIESAIGEIIPGTLISGPAGQILGGLSLQQTPVN
EEEECCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCC
HKYVIPEDWKKRFPSGNEIIQYAAGHYAKNSKDPDEQLIDRRRVEYDIFLLVEELHVLDI
CEEECCCHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
IKKGFDSVDEFIALANSVSNRRKSRAGKSLELHLEKLFIEHGLRHFSTQAVTEGNKKPDF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
LFPSAEAYHNVEFPVENLRMLAVKTTCKDRWRQILNEADKIHQVHLFTLQEGVSSAQYRE
ECCCHHHCCCCCCCHHHEEEEEEHHHHHHHHHHHHHHHHHHHEEEEEEHHHCCCHHHHHH
MKDAGVRLVVPSTLHKKYPEAVREELITLGAFITELIELYAELS
HHHCCEEEEECHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYMSVFHNWLLDIASGNYFIYIKRLSANDTGATGGHQVGLYIPSNIVEKLFPSINHTREL
CHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCEEEEEECHHHHHHHCCCCCCCCCC
NPSVFLTAHVSSHDCPDTQARAIYYNNRYFGKTRNEKRITRWGRGSPLQNPENTGALTIL
CCCEEEEEECCCCCCCCCCCEEEEECCEEECCCCCCHHHHHCCCCCCCCCCCCCCEEEEE
AFRLNEQNTDCSEVDIWVCVNPDEEDIIESAIGEIIPGTLISGPAGQILGGLSLQQTPVN
EEEECCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCC
HKYVIPEDWKKRFPSGNEIIQYAAGHYAKNSKDPDEQLIDRRRVEYDIFLLVEELHVLDI
CEEECCCHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
IKKGFDSVDEFIALANSVSNRRKSRAGKSLELHLEKLFIEHGLRHFSTQAVTEGNKKPDF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
LFPSAEAYHNVEFPVENLRMLAVKTTCKDRWRQILNEADKIHQVHLFTLQEGVSSAQYRE
ECCCHHHCCCCCCCHHHEEEEEEHHHHHHHHHHHHHHHHHHHEEEEEEHHHCCCHHHHHH
MKDAGVRLVVPSTLHKKYPEAVREELITLGAFITELIELYAELS
HHHCCEEEEECHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2104830; 2597679; 2612358; 8392701 [H]