Click here to switch to the map view.

The map label for this gene is pheA

Identifier: 218701111

GI number: 218701111

Start: 2915052

End: 2916212

Strand: Direct

Name: pheA

Synonym: ECIAI39_2803

Alternate gene names: 218701111

Gene position: 2915052-2916212 (Clockwise)

Preceding gene: 218701110

Following gene: 218701114

Centisome position: 56.8

GC content: 48.84

Gene sequence:

>1161_bases
ATGACATCGGAAAACCCGTTACTGGCGCTGCGAGAGAAAATCAGCGCGCTGGATGAAAAATTATTAGCATTACTGGCAGA
GCGACGCGAACTGGCCGTCGAGGTGGGAAAAGCCAAACTGCTCTCGCATCGCCCGGTACGTGATATTGATCGCGAACGCG
ATTTGCTGGAAAGATTAATTACGCTCGGTAAAGCGCACCATCTGGACGCCCATTACATTACTCGCCTGTTCCAGCTCATC
ATTGAAGATTCCGTATTAACTCAGCAGGCACTGCTCCAGCAACATCTCAATAAAATTAATCCGCACTCAGCACGCATCGC
TTTTCTCGGCCCCAAAGGCTCCTATTCACATCTTGCCGCTCGTCAGTACGCTGCGCGTCACTTTGAGCAATTCATTGAAA
GTGGCTGCGCCAAATTTGCCGATATTTTTAATCAGGTGGAAACCGGCCAGGCCGACTATGCCGTCGTACCGATTGAAAAT
ACCAGCTCCGGTGCCATAAACGACGTTTACGATCTGCTGCAACATACCAGTTTGTCGATTGTTGGCGAGATGACGTTAAC
TATCGATCATTGTTTGTTGGTCTCCGGCACTACTGATTTATCCACCATTAATACGGTCTACAGCCATCCGCAGCCATTCC
AGCAATGCAGCAAATTCCTTAATCGTTATCCGCACTGGAAGATTGAATATACCGAAAGTACGTCTGCGGCAATGGAAAAG
GTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGA
GCGTATTGAAGCGAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCACGTAAAGCCATTAACGTTTCTGACCAGG
TTCCGGCGAAAACGACGTTGTTAATGGCGACCGGACAACAAGCTGGTGCACTGGTTGAAGCGTTGCTGGTACTGCGCAAC
CACAGTCTAATTATGACCCGTCTGGAATCACGTCCGATTCACGGTAATCCGTGGGAAGAGATGTTTTATCTGGATATTCA
GGCCAATCTTGAATCAGCGGAAATGCAAAAAGCATTGAAAGAGTTAGGGGAAATCACCCGTTCGATGAAGGTATTGGGCT
GTTACCCAAGTGAGAACGTAGTGCCTGTTGATCCAACCTGA

Upstream 100 bases:

>100_bases
GAATGGGAGGCGTTTCGTCGTGTGAAACAGAATGCGAAGACGAACAATAAGGCCTCCCAAATCGGGGGGCCTTTTTTATT
GATAACAAAAAGGCAACACT

Downstream 100 bases:

>100_bases
TGAAAAAGTGCCGGATGATGTGAATCATCCGGCACTGGATTATTACTGGCGATTGTCATTCGCCTGACGCAATAACACGC
GGCTTTCACTCTGAAAACGC

Product: bifunctional chorismate mutase/prephenate dehydratase

Products: NA

Alternate protein names: Chorismate mutase; CM; Prephenate dehydratase; PDT [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLI
IEDSVLTQQALLQQHLNKINPHSARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEK
VAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQNFTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRN
HSLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT

Sequences:

>Translated_386_residues
MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLI
IEDSVLTQQALLQQHLNKINPHSARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEK
VAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQNFTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRN
HSLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT
>Mature_385_residues
TSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLII
EDSVLTQQALLQQHLNKINPHSARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIENT
SSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEKV
AQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQNFTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNH
SLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT

Specific function: L-phenylalanine biosynthesis. [C]

COG id: COG0077

COG function: function code E; Prephenate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 prephenate dehydratase domain [H]

Homologues:

Organism=Escherichia coli, GI1788951, Length=386, Percent_Identity=99.7409326424871, Blast_Score=798, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324013, Length=323, Percent_Identity=26.9349845201238, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008242
- InterPro:   IPR002701
- InterPro:   IPR020822
- InterPro:   IPR010952
- InterPro:   IPR001086
- InterPro:   IPR018528 [H]

Pfam domain/function: PF01817 CM_2; PF00800 PDT [H]

EC number: =5.4.99.5; =4.2.1.51 [H]

Molecular weight: Translated: 43085; Mature: 42954

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00857 PREPHENATE_DEHYDR_1 ; PS00858 PREPHENATE_DEHYDR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDIDRERDLLERLI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPHSARIAFLGPKGSYSHLAA
HHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
RQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIENTSSGAINDVYDLLQHTSLSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHH
VGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEK
HHHHHEEEEHEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
VAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQNFTRFVVLARKAINVSDQVPAKTTL
HHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEE
LMATGQQAGALVEALLVLRNHSLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALK
EEECCCHHHHHHHHHHHHHCCHHEEEHHHCCCCCCCCHHHEEEEEEECCCCHHHHHHHHH
ELGEITRSMKVLGCYPSENVVPVDPT
HHHHHHHHHHHEEECCCCCEEECCCC
>Mature Secondary Structure 
TSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHRPVRDIDRERDLLERLI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPHSARIAFLGPKGSYSHLAA
HHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
RQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIENTSSGAINDVYDLLQHTSLSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHH
VGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSKFLNRYPHWKIEYTESTSAAMEK
HHHHHEEEEHEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
VAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQNFTRFVVLARKAINVSDQVPAKTTL
HHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEE
LMATGQQAGALVEALLVLRNHSLIMTRLESRPIHGNPWEEMFYLDIQANLESAEMQKALK
EEECCCHHHHHHHHHHHHHCCHHEEEHHHCCCCCCCCHHHEEEEEEECCCCHHHHHHHHH
ELGEITRSMKVLGCYPSENVVPVDPT
HHHHHHHHHHHEEECCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]