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The map label for this gene is yfhQ

Identifier: 218701042

GI number: 218701042

Start: 2835781

End: 2836521

Strand: Reverse

Name: yfhQ

Synonym: ECIAI39_2733

Alternate gene names: 218701042

Gene position: 2836521-2835781 (Counterclockwise)

Preceding gene: 218701046

Following gene: 218701041

Centisome position: 55.27

GC content: 54.25

Gene sequence:

>741_bases
ATGCTGCAAAATATTCGAATTGTGCTGGTGGAGACGTCACACACCGGCAATATGGGTTCTGTTGCCCGTGCCATGAAAAC
AATGGGATTAACCAATCTGTGGCTGGTTAATCCACTGGTGAAACCTGACTCCCAGGCGATTGCCCTGGCAGCAGGGGCCA
GCGATGTGATTGGTAATGCTCACATCGTCGATACGCTCGACGAAGCGTTAGCCGGTTGTAGCCTGGTGGTGGGCACCAGC
GCACGCTCCCGCACGCTGCCGTGGCCGATGCTCGACCCGCGCGAATGCGGTCTGAAAAGTGTCGCTGAAGCGGCAAATAC
CCCGGTGGCGCTGGTTTTTGGTCGCGAGCGCGTAGGCTTGACCAATGAAGAGTTGCAGAAATGCCATTATCATGTCGCGA
TTGCTGCGAACCCGGAATACAGTTCGCTGAACCTGGCGATGGCGGTTCAGGTTATTGCCTATGAAGTGCGCATGGCCTGG
CTGGCAACTCAGGAAAATGGCGAACAAGTCGAGCATGAAGAGACGCCGTATCCGCTGGTAGATGATCTGGAGCGTTTTTA
CGGTCATCTCGAACAAACGCTGCGGGCAACCGGTTTTATCCGTGAAAACCATCCGGGGCAGGTGATGAATAAATTGCGCC
GTCTGTTTACCCGTGCACGCCCGGAAAGCCAGGAATTGAATATCCTGCGCGGGATTCTGGCTTCTATAGAGCAGCAGAAT
AAAGGTAATAAGGCCGAATAA

Upstream 100 bases:

>100_bases
GTTAAAGAACGGGAAAACGGCGCAGAGTATAGCAGCGAGATCGGCATCTGTCTTCGTTTTATGATAAGATGCGTGAATAA
TCTTTAGATGAAGAAAGACA

Downstream 100 bases:

>100_bases
CAGTGGTTGCCTGATGCGATGCTTAACGCGTCTTATCAGGCTTGTAAGTGCACGGAATCCCAGGTCGGATAAGGTGTTCA
CACCGCATCCGACAACAGGT

Product: putative methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLRATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
KGNKAE

Sequences:

>Translated_246_residues
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLRATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
KGNKAE
>Mature_246_residues
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLRATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
KGNKAE

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=246, Percent_Identity=99.5934959349593, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1790865, Length=153, Percent_Identity=37.2549019607843, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27091; Mature: 27091

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNA
CCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCEEEEECCCHHHCCCC
HIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGL
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHCCCEEEEECCCCCCC
TNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWLATQENGEQVEHEETPYPLV
CHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHH
DDLERFYGHLEQTLRATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
KGNKAE
CCCCCC
>Mature Secondary Structure
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNA
CCCCEEEEEEEECCCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCEEEEECCCHHHCCCC
HIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVAEAANTPVALVFGRERVGL
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHCCHHHHHHHHCCCEEEEECCCCCCC
TNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWLATQENGEQVEHEETPYPLV
CHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHH
DDLERFYGHLEQTLRATGFIRENHPGQVMNKLRRLFTRARPESQELNILRGILASIEQQN
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
KGNKAE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA