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The map label for this gene is dnaT [H]

Identifier: 218701004

GI number: 218701004

Start: 2785665

End: 2786498

Strand: Reverse

Name: dnaT [H]

Synonym: ECIAI39_2694

Alternate gene names: 218701004

Gene position: 2786498-2785665 (Counterclockwise)

Preceding gene: 218701005

Following gene: 218701003

Centisome position: 54.3

GC content: 47.48

Gene sequence:

>834_bases
ATGAGCAGACATGCAACAGATTGGGCCTGGGAGACAGATCCAGGTAGCTCATCATTAAAGCTCATACTGCTCTCGATGGC
TGACAGAGCCGATGAATATAACCTCTGCTACCCCAGCATAGAACGCCTCGTTAAAGACACTTGCCTGAATAAAAAAACCG
TGCAGGCCGGGCTTATATCGCTCATGAAAATGGGGCTTATTTCAGATACCGGAGAGAGAAAGGGAGCGACAAAAAGAGTG
CGGGTTTTCTCTCTTAATATAACCAAAAACGGGAACATTAAAGGCAACCGGGAGGGGGGCAATGAACCCGAAAACGGTAA
TGTTACCGAAAACGGGAATATACCCAAAAACGGGATGTTGAATGATCCCAAAAACGGGATGTTGAATGATCCCAAAAACG
GGATCCAGAACCAGTCATATAACCAGTCATTTAACCAAGAGAGGGAGAGCAGGACAAAAACCGGGGATTCTGTTCCTCAT
GACCCCGGCGCAAACAACGCCGTGATGAATAACTTTGTTCCTCCTGGTGGGCCAGGGCAATTAGGCAAATTTGTCATGCA
TGAACAATGGCAGCCATCAGATGACTTTCTTCGGAAAAGCTCATTGCAGGGAATCTACCTGGACAGTCTGCCAACGGCAC
AGGAACTTGCAGAGTTCAGAATTTACTGGATGGCTGAGGGTAAGGCATACCATCAGGCACAGTGGGAGCAGAAGCTGGCA
AGGCGCATCCAGTTCTGTAGACAGAAATCAGGTGAAGTCAGAAAGAGCCTTGACTGGCATAACACTGACTGGATAGATGA
GGTGTGGGATGAAATCAACTCCAGAACTTCTTAA

Upstream 100 bases:

>100_bases
TGAGTGCACCATGGAATCAAACCGTCGCATCAGGGCAGAGATTAAGCAGATCATGATTAATTCCTCCCCACAACACTCAA
GCTGATAGCGGAGATTAATC

Downstream 100 bases:

>100_bases
TGAGTACGATCGCTTTCGTGGTGGGCATGACCACTCGACGGCGGTTGCTTCTGCTGACGATGAAAGGGCAAAGAAGGAGC
AGGTTGCAAGAATTTTCAAC

Product: putative primosomal protein from phage

Products: NA

Alternate protein names: Primosomal protein I [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSRHATDWAWETDPGSSSLKLILLSMADRADEYNLCYPSIERLVKDTCLNKKTVQAGLISLMKMGLISDTGERKGATKRV
RVFSLNITKNGNIKGNREGGNEPENGNVTENGNIPKNGMLNDPKNGMLNDPKNGIQNQSYNQSFNQERESRTKTGDSVPH
DPGANNAVMNNFVPPGGPGQLGKFVMHEQWQPSDDFLRKSSLQGIYLDSLPTAQELAEFRIYWMAEGKAYHQAQWEQKLA
RRIQFCRQKSGEVRKSLDWHNTDWIDEVWDEINSRTS

Sequences:

>Translated_277_residues
MSRHATDWAWETDPGSSSLKLILLSMADRADEYNLCYPSIERLVKDTCLNKKTVQAGLISLMKMGLISDTGERKGATKRV
RVFSLNITKNGNIKGNREGGNEPENGNVTENGNIPKNGMLNDPKNGMLNDPKNGIQNQSYNQSFNQERESRTKTGDSVPH
DPGANNAVMNNFVPPGGPGQLGKFVMHEQWQPSDDFLRKSSLQGIYLDSLPTAQELAEFRIYWMAEGKAYHQAQWEQKLA
RRIQFCRQKSGEVRKSLDWHNTDWIDEVWDEINSRTS
>Mature_276_residues
SRHATDWAWETDPGSSSLKLILLSMADRADEYNLCYPSIERLVKDTCLNKKTVQAGLISLMKMGLISDTGERKGATKRVR
VFSLNITKNGNIKGNREGGNEPENGNVTENGNIPKNGMLNDPKNGMLNDPKNGIQNQSYNQSFNQERESRTKTGDSVPHD
PGANNAVMNNFVPPGGPGQLGKFVMHEQWQPSDDFLRKSSLQGIYLDSLPTAQELAEFRIYWMAEGKAYHQAQWEQKLAR
RIQFCRQKSGEVRKSLDWHNTDWIDEVWDEINSRTS

Specific function: This protein is required for primosome-dependent normal DNA replication; it is also involved in inducing stable DNA replication during SOS response. It forms, in concert with dnaB protein and other prepriming proteins dnaC, N, N', N'' a prepriming protein

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaT family [H]

Homologues:

Organism=Escherichia coli, GI1790824, Length=75, Percent_Identity=48, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020917 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 31333; Mature: 31202

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRHATDWAWETDPGSSSLKLILLSMADRADEYNLCYPSIERLVKDTCLNKKTVQAGLIS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LMKMGLISDTGERKGATKRVRVFSLNITKNGNIKGNREGGNEPENGNVTENGNIPKNGML
HHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NDPKNGMLNDPKNGIQNQSYNQSFNQERESRTKTGDSVPHDPGANNAVMNNFVPPGGPGQ
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCHH
LGKFVMHEQWQPSDDFLRKSSLQGIYLDSLPTAQELAEFRIYWMAEGKAYHQAQWEQKLA
HHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCHHHHHHEEEEEEECCCHHHHHHHHHHHH
RRIQFCRQKSGEVRKSLDWHNTDWIDEVWDEINSRTS
HHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRHATDWAWETDPGSSSLKLILLSMADRADEYNLCYPSIERLVKDTCLNKKTVQAGLIS
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LMKMGLISDTGERKGATKRVRVFSLNITKNGNIKGNREGGNEPENGNVTENGNIPKNGML
HHHHHHHCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NDPKNGMLNDPKNGIQNQSYNQSFNQERESRTKTGDSVPHDPGANNAVMNNFVPPGGPGQ
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCHH
LGKFVMHEQWQPSDDFLRKSSLQGIYLDSLPTAQELAEFRIYWMAEGKAYHQAQWEQKLA
HHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCHHHHHHEEEEEEECCCHHHHHHHHHHHH
RRIQFCRQKSGEVRKSLDWHNTDWIDEVWDEINSRTS
HHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA