Click here to switch to the map view.

The map label for this gene is cysP

Identifier: 218700891

GI number: 218700891

Start: 2666508

End: 2667524

Strand: Reverse

Name: cysP

Synonym: ECIAI39_2570

Alternate gene names: 218700891

Gene position: 2667524-2666508 (Counterclockwise)

Preceding gene: 218700892

Following gene: 218700890

Centisome position: 51.98

GC content: 53.69

Gene sequence:

>1017_bases
ATGGCCGTTAACTTACTGAAAAAGAACTCACTCGCGCTGGTCGCTTCTCTGCTGCTGGCGGGCCATGTACAGGCAACGGA
ACTGCTGAACAGTTCTTATGACGTCTCCCGCGAGCTGTTTGCCGCCCTGAATCCGCCGTTTGAGCAACAATGGGCAAAAG
ATAACGGCGGCGACAAACTGACGATTAAACAATCTCATGCCGGGTCATCAAAACAGGCGCTGGCGATTTTACAGGGCTTA
AAAGCGGACGTTGTCACTTATAACCAGGTGACTGACGTACAAATCCTGCATGACAAAGGCAAGCTTATCCCGGCGGACTG
GCAAACGCGCCTGCCGAATAACAGCTCGCCGTTCTACTCCACCATGGGCTTCCTGGTGCGTAAGGGCAACCCGAAGAATA
TCCACGACTGGAACGATCTGGTGCGCTCCGACGTGAAGCTGATTTTCCCGAACCCGAAAACGTCGGGTAACGCGCGTTAT
ACCTATCTGGCGGCATGGGGCGCAGCGGATAAAGCTGACGGTGGCGACAAAGCCAAAACCGAACAGTTTATGACCCAGTT
CCTGAAAAACGTTGAAGTGTTCGATACTGGCGGTCGTGGCGCGACCACCACTTTTGCCGAGCGCGGCCTGGGCGATGTGC
TGATCAGCTTCGAGTCGGAAGTGAACAACATCCGCAAACAGTATGAAGCGCAGGGCTTTGAAGTGGTGATTCCGAAAACC
AATATTCTGGCGGAATTCCCGGTGGCGTGGGTCGATAAAAACGTGCAGGCCAACGGTACGGAAAAAGCAGCAAAAGCTTA
CCTGAACTGGCTCTACAGCCCGCAGGCGCAAACCATCATCACCGACTATTACTACCGCGTAAATAACCCGGAGGTGATGG
ACAAACTGAAAGACAAATTCCCGCAGACCGAACTGTTCCGCGTGGAAGACAAATTTGGCTCCTGGCCGGAAGTGATGAAA
ACCCACTTCACCAGCGGCGGCGAGTTAGACAAGCTGTTAGCGGCGGGGCGTAAGTAA

Upstream 100 bases:

>100_bases
ACTTCCAAATCACCAAACGGTATATAAAACCGTTACTCCTTTCACGTCCGTTATAAATATGATGGCTATTAGAAAGTCAT
TAAATTTATAAGGGTGCGCA

Downstream 100 bases:

>100_bases
TGTTTGCAGTTTCTTCAAGACGCGTGCTGCCGGGCTTTACCTTAAGCCTCGGCACCAGTCTGCTGTTTGTGTGCCTGATT
TTGCTGCTGCCGCTCTCCGC

Product: thiosulfate transporter subunit

Products: SO42- [Cytoplasm]; phosphate; ADP; S2O32- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALNPPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGL
KADVVTYNQVTDVQILHDKGKLIPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQYEAQGFEVVIPKT
NILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMK
THFTSGGELDKLLAAGRK

Sequences:

>Translated_338_residues
MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALNPPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGL
KADVVTYNQVTDVQILHDKGKLIPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQYEAQGFEVVIPKT
NILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMK
THFTSGGELDKLLAAGRK
>Mature_337_residues
AVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALNPPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLK
ADVVTYNQVTDVQILHDKGKLIPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARYT
YLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQYEAQGFEVVIPKTN
ILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKT
HFTSGGELDKLLAAGRK

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. This protein specifically binds thiosulfate and is involved in its transmembrane transport [H]

COG id: COG4150

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1788765, Length=337, Percent_Identity=99.406528189911, Blast_Score=692, Evalue=0.0,
Organism=Escherichia coli, GI1790351, Length=323, Percent_Identity=46.4396284829721, Blast_Score=301, Evalue=4e-83,

Paralogues:

None

Copy number: 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37657; Mature: 37526

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS00092 N6_MTASE ; PS00401 PROK_SULFATE_BIND_1 ; PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALNPPFEQQWAKDNGGDKL
CCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHCCCCCCCEE
TIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKLIPADWQTRLPNNSSPFYS
EEECCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCEECCCHHHCCCCCCCCHHH
TMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARYTYLAAWGAADKADGGDKAKT
HHHHHEECCCCCCCCHHHHHHHCCCEEEECCCCCCCCCEEEEEEEECCCCCCCCCCHHHH
EQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQYEAQGFEVVIPKT
HHHHHHHHCCCEEEECCCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCEEEECCC
NILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQTIITDYYYRVNNPEVMDKLKDKF
CCHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHHC
PQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLAAGRK
CCCCEEEEHHHCCCCHHHHHHHCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
AVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALNPPFEQQWAKDNGGDKL
CCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHHCCCCCCCEE
TIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKLIPADWQTRLPNNSSPFYS
EEECCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEEECCCCEECCCHHHCCCCCCCCHHH
TMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARYTYLAAWGAADKADGGDKAKT
HHHHHEECCCCCCCCHHHHHHHCCCEEEECCCCCCCCCEEEEEEEECCCCCCCCCCHHHH
EQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFESEVNNIRKQYEAQGFEVVIPKT
HHHHHHHHCCCEEEECCCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCEEEECCC
NILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQAQTIITDYYYRVNNPEVMDKLKDKF
CCHHHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHHHHHHHHC
PQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLAAGRK
CCCCEEEEHHHCCCCHHHHHHHCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: SO42- [Periplasm]; H2O; ATP; S2O32- [Periplasm] [C]

Specific reaction: SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188959; 9205837; 9278503; 9298646 [H]