Click here to switch to the map view.

The map label for this gene is vacJ

Identifier: 218700824

GI number: 218700824

Start: 2586441

End: 2587196

Strand: Reverse

Name: vacJ

Synonym: ECIAI39_2499

Alternate gene names: 218700824

Gene position: 2587196-2586441 (Counterclockwise)

Preceding gene: 218700829

Following gene: 218700821

Centisome position: 50.41

GC content: 52.51

Gene sequence:

>756_bases
ATGAAGCTTCGCCTGTCGGCGCTTGCTCTGGGAACTACGCTTCTGGTGGGGTGTGCGAGTTCCGGTACAGATCAGCAAGG
GCGTTCTGACCCGTTAGAAGGGTTCAACCGCACCATGTACAACTTCAACTTCAATATATTAGACCCGTATATTGTTCGAC
CGGTCGCTGTCGCCTGGCGTGATTATGTTCCGCAACCGGCGCGTAACGGTTTGAGCAACTTTACTGGCAACCTTGAAGAA
CCTGCGGTGATGGTTAACTACTTCTTGCAGGGCGACCCTTATCAGGGGATGGTCCACTTTACCCGCTTTTTCCTGAACAC
CATTTTGGGGATGGGCGGCTTTATTGATGTTGCAGGGATGGCGAACCCGAAACTGCAACGGACTGAACCTCACCGCTTCG
GTAGTACGCTTGGTCATTATGGTGTGGGTTATGGGCCATACGTTCAGTTACCGTTCTACGGTAGCTTCACGCTGCGTGAT
GACGGTGGTGATATGGCGGATGGTCTTTACCCGGTTCTTTCCTGGCTGACCTGGCCGATGTCTGTGGGTAAATGGACGCT
TGAAGGGATAGAAACTCGTGCGCAGTTGTTGGATTCCGACGGCCTGCTGCGTCAGTCGTCCGACCCATACATTATGGTGC
GCGAAGCGTACTTCCAGCGTCATGATTTCATCGCCAATGGCGGCGAACTCAAACCGCAGGAAAACCCGAACGCGCAAGCG
ATTCAGGATGAGTTAAAAGATATCGATTCTGAATAA

Upstream 100 bases:

>100_bases
GACGTGCTGTTACAATCGCCCACACCTAAACAGGCGGATACGGTATCGTTCCGTCATGGATGGCAAACTGCATAAGCCAT
AAAAAAACAGGGAGACATTT

Downstream 100 bases:

>100_bases
GAAACAAATAAAAAAGGTGAGTCACAAAACTCACCTTTTTTGTTGTTATCCCTCAATTACCCCAAGTAAAAGAAGATAAA
CCGACAATGACCTGGACATA

Product: putative lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNFNILDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEE
PAVMVNYFLQGDPYQGMVHFTRFFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGGELKPQENPNAQA
IQDELKDIDSE

Sequences:

>Translated_251_residues
MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNFNILDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEE
PAVMVNYFLQGDPYQGMVHFTRFFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGGELKPQENPNAQA
IQDELKDIDSE
>Mature_251_residues
MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNFNILDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEE
PAVMVNYFLQGDPYQGMVHFTRFFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGGELKPQENPNAQA
IQDELKDIDSE

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=251, Percent_Identity=98.804780876494, Blast_Score=515, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 28036; Mature: 28036

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNFNILDPYIVRPVAVAWR
CCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCEEEEECCEEECHHHHHHHHHHHH
DYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTRFFLNTILGMGGFIDVAGM
HHCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEECCC
ANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRDDGGDMADGLYPVLSWLTWPM
CCCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHH
SVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGGELKPQENPNAQA
HCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCHHH
IQDELKDIDSE
HHHHHHHCCCC
>Mature Secondary Structure
MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNFNILDPYIVRPVAVAWR
CCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCEEEEECCEEECHHHHHHHHHHHH
DYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTRFFLNTILGMGGFIDVAGM
HHCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEECCC
ANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRDDGGDMADGLYPVLSWLTWPM
CCCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHH
SVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYFQRHDFIANGGELKPQENPNAQA
HCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCHHH
IQDELKDIDSE
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]