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The map label for this gene is yfcU

Identifier: 218700816

GI number: 218700816

Start: 2574511

End: 2577168

Strand: Reverse

Name: yfcU

Synonym: ECIAI39_2490

Alternate gene names: 218700816

Gene position: 2577168-2574511 (Counterclockwise)

Preceding gene: 218700817

Following gene: 218700815

Centisome position: 50.22

GC content: 54.29

Gene sequence:

>2658_bases
ATGTGTATGCCTAATCACTCAAATTTTCGGCTGCGGGGAATCGCCTGCTATATTGCGCTGGCAATCTCTGGTGGATCAGT
CAATGCATGGGCTGATGATTCCATTCAATTTGACCCCCGTTTCCTTGAGTTAAAGGGTGACACGAAAATTGATCTCGGTA
AGTTTTCAAAAAAAGGGTATGTCGACGCGGGTAAATATAATTTACGTGTATTTATAAATAAACAACCCCTTTCTGATGAA
TACGACATTAACTGGTACGTCTCTGAAAATGATCCAACAAAAACTTATGCCTGCCTGACACCTGAGTTAGTGGCGGCGCT
GGGGCTGAAAGAAGGGATAGCAAAAAGCCTGCAGTGGACGCACAACGATGAATGCCTTAAACCGGGGCAATTAGATGGGA
TGGAAGTCGAGAATGATTTAAGCCAGTCGGCGTTGCTGCTGACAGTGCCACAGGCTTATCTCGAATATACCAGCAGCGAC
TGGGACCCACCCTCACGCTGGGACGACGGTATTCCTGGCCTGATTGCCGACTACAGCCTCAATGCGCAAACCCGTCATCA
GGAGCAGGGTGGCGAGGACTCACATGATATCAGCGGCAACGGTACCGTTGGGGCGAATCTGGGGGCATGGCGTTTCCGCG
CAGACTGGCAAAGTGATTATCAGCACACCCGCAGCAACGACGACGAAGACGATGACAGCAGTAACAGTACAACGAGCAAA
AACTGGGACTGGAGCCGTTATTACGCCTGGCGGGCCTTACCCTCTTTAAAAGCCAAGCTGTCGCTGGGTGAAGATTATCT
CAACTCCGATATTTTCGACGGCTTTAACTATATTGGCAGCAGCGTCAGCACCGATGACCAGATGCTGCCGCCCAACCTGC
GCGGCTATGCGCCGGATGTCTCCGGCGTGGCGCACAGCAGTGCAAAAGTCACTATTAGCCAGATGGGCCGGGTACTTTAC
GAAACCCAGGTTCCCGCCGGGCCATTCCGCATTCAGGATATCGGCGACTCCGTCTCCGGCACACTGCACGTCCGCGTTGA
AGAACAGAATGGTCAGGTGCAGGAATATGACGTTACGACCGCATCTATGCCATTCCTCACGCGCCAGGGGCAGGTGCGTT
ACAAAGTGATGATGGGGCGTCCGGAAGACTGGAACCACAAGACCGAAGGCGGCTTTTTCTCCGGCGGAGAAGCGTCATGG
GGGGTGGCAGATGGCTGGTCGCTCTACGGTGGCGCGCTGGCAGATGAACACTACCAGTCGGCGGCGATGGGGGTGGGGCG
CGACCTCGCACAGTTTGGCGCGCTGGCGTTCGATGTGACTCACTCGCACGTCAACCTGGATCATGACAGCGCATACGGCA
AAGGAAAACTGGACGGCAACTCCTTTCGCGTGAGCTATGCCAAAGACTTTGACGAACTTAACAGCCGCGTCACCTTTGCA
GGCTACCGTTTTTCTGAAAAGAACTTCATGACCATGAGCGAGTATCTGGACGCGAACCAGTCGGACATGGCGCGGACCGG
TAACGACAAAGAGATGTATACGATCACCTATAACCAGAACTTTGCCGCTGCGGGTGTCTCGATCTATCTCAACTACTCCC
ATCGTACTTACTGGGATCGCCCGGAACAGACAAACTATAACCTGATGTTTTCCCACTATTTTAATATGGGGAGTATTCGC
AACATGAGCATCTCGGTGACCGGCTATCGCTACGAATATGACGATAACGCGGATAAGGGGATGTACCTCAGCATGAGCAT
TCCGTGGAGCGACAGCAGCACCGTGACCTACAACGGCTCCTACGGCAGTGGGTCGGACAGCAGCCAGGTCGGTTACTTTA
AGCGCGTTGATGACGCAACGCACTACCAGGTTAACGTCGGTACCAGCGAACAGCACGGCAGCGTGGATGGTTATCTGAGT
CACGACGGCTCGCTGGCGAAGGTTGATCTCAGCGCCAACTACCATGAGGGGGAATACCGCTCGGCGGGGATCGCCTTACA
GGGCGGGGCAACGCTGACCGCGCATGGTGGGGCGCTGCATCGCACTCAAAGCATGGGCGGTACGCGCTTGCTGATTGACG
CCGACGGGATTGCCAATGTGCCGGTCGAAAGCAACGGCGCGCCGGTGTACACCAATATGTTTGGCAAGGCGGTGGTCGCC
GATATTAACAACTACTATCGCAATCAGGCGTATATCGATTTAAACAACCTGCCAGAAGATGCGGAAGCCACCCAGTCGGT
GGTACAGGCAACCCTGACGGAAGGTGCAATTGGCTATCGCAAATTCAAAGTGATCAGCGGGCAAAAAGCGATGGCGGTAC
TGCGGTTGCGTGACGGCAGCTACCCGCCGTTTGGCGCGGAAGTGAAAAACGACGAGCAGCAGCAGGTTGGCATTGTGGAT
GATGAAGGCAATGTCTATCTGGCGGGAGTCAATGCGGGTGAGCATATGACGGTGTTCTGGGAAGGCAGCGCACAATGCGA
GATCGTATTGCCGAAGCCGCTACCTGCCGATCTGTTCAGCGGCCTGTTGTTGCCGTGTGAACAAAAGGGAACGGCAGCCC
CTGATTCTTCAGCGCCAGAAATTAAGCCTGTTATTCAGGACCAGACGCGGCAAGTCACACCAACGGAAGCGCCGACGTCA
ATTTCAGCTACTCAATAA

Upstream 100 bases:

>100_bases
CCACATTCCAGATTACCTATCTCTGATGTGACAACCCCACAGCGTTCTGCTGTGGGGTTTTTCAACGCGCAACGGATTTA
CGACTGTTTCACAAACGGAT

Downstream 100 bases:

>100_bases
TCTGATTAAGGAACGATCAATGTCTTATAAATTATCCAGTTCAATGGTCGTCAGCACGGCGCTGGCGGCACTGCTTTCCA
CCGTCAGCCTGGCGGCGCAC

Product: putative export usher protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 885; Mature: 885

Protein sequence:

>885_residues
MCMPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFDPRFLELKGDTKIDLGKFSKKGYVDAGKYNLRVFINKQPLSDE
YDINWYVSENDPTKTYACLTPELVAALGLKEGIAKSLQWTHNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSD
WDPPSRWDDGIPGLIADYSLNAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDYQHTRSNDDEDDDSSNSTTSK
NWDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIGSSVSTDDQMLPPNLRGYAPDVSGVAHSSAKVTISQMGRVLY
ETQVPAGPFRIQDIGDSVSGTLHVRVEEQNGQVQEYDVTTASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSGGEASW
GVADGWSLYGGALADEHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYGKGKLDGNSFRVSYAKDFDELNSRVTFA
GYRFSEKNFMTMSEYLDANQSDMARTGNDKEMYTITYNQNFAAAGVSIYLNYSHRTYWDRPEQTNYNLMFSHYFNMGSIR
NMSISVTGYRYEYDDNADKGMYLSMSIPWSDSSTVTYNGSYGSGSDSSQVGYFKRVDDATHYQVNVGTSEQHGSVDGYLS
HDGSLAKVDLSANYHEGEYRSAGIALQGGATLTAHGGALHRTQSMGGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVA
DINNYYRNQAYIDLNNLPEDAEATQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVGIVD
DEGNVYLAGVNAGEHMTVFWEGSAQCEIVLPKPLPADLFSGLLLPCEQKGTAAPDSSAPEIKPVIQDQTRQVTPTEAPTS
ISATQ

Sequences:

>Translated_885_residues
MCMPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFDPRFLELKGDTKIDLGKFSKKGYVDAGKYNLRVFINKQPLSDE
YDINWYVSENDPTKTYACLTPELVAALGLKEGIAKSLQWTHNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSD
WDPPSRWDDGIPGLIADYSLNAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDYQHTRSNDDEDDDSSNSTTSK
NWDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIGSSVSTDDQMLPPNLRGYAPDVSGVAHSSAKVTISQMGRVLY
ETQVPAGPFRIQDIGDSVSGTLHVRVEEQNGQVQEYDVTTASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSGGEASW
GVADGWSLYGGALADEHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYGKGKLDGNSFRVSYAKDFDELNSRVTFA
GYRFSEKNFMTMSEYLDANQSDMARTGNDKEMYTITYNQNFAAAGVSIYLNYSHRTYWDRPEQTNYNLMFSHYFNMGSIR
NMSISVTGYRYEYDDNADKGMYLSMSIPWSDSSTVTYNGSYGSGSDSSQVGYFKRVDDATHYQVNVGTSEQHGSVDGYLS
HDGSLAKVDLSANYHEGEYRSAGIALQGGATLTAHGGALHRTQSMGGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVA
DINNYYRNQAYIDLNNLPEDAEATQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVGIVD
DEGNVYLAGVNAGEHMTVFWEGSAQCEIVLPKPLPADLFSGLLLPCEQKGTAAPDSSAPEIKPVIQDQTRQVTPTEAPTS
ISATQ
>Mature_885_residues
MCMPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFDPRFLELKGDTKIDLGKFSKKGYVDAGKYNLRVFINKQPLSDE
YDINWYVSENDPTKTYACLTPELVAALGLKEGIAKSLQWTHNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSD
WDPPSRWDDGIPGLIADYSLNAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDYQHTRSNDDEDDDSSNSTTSK
NWDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIGSSVSTDDQMLPPNLRGYAPDVSGVAHSSAKVTISQMGRVLY
ETQVPAGPFRIQDIGDSVSGTLHVRVEEQNGQVQEYDVTTASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSGGEASW
GVADGWSLYGGALADEHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYGKGKLDGNSFRVSYAKDFDELNSRVTFA
GYRFSEKNFMTMSEYLDANQSDMARTGNDKEMYTITYNQNFAAAGVSIYLNYSHRTYWDRPEQTNYNLMFSHYFNMGSIR
NMSISVTGYRYEYDDNADKGMYLSMSIPWSDSSTVTYNGSYGSGSDSSQVGYFKRVDDATHYQVNVGTSEQHGSVDGYLS
HDGSLAKVDLSANYHEGEYRSAGIALQGGATLTAHGGALHRTQSMGGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVA
DINNYYRNQAYIDLNNLPEDAEATQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVGIVD
DEGNVYLAGVNAGEHMTVFWEGSAQCEIVLPKPLPADLFSGLLLPCEQKGTAAPDSSAPEIKPVIQDQTRQVTPTEAPTS
ISATQ

Specific function: Involved in the export and assembly of a fimbrial subunit across the outer membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fimbrial export usher family [H]

Homologues:

Organism=Escherichia coli, GI87081778, Length=848, Percent_Identity=35.4952830188679, Blast_Score=497, Evalue=1e-141,
Organism=Escherichia coli, GI1790772, Length=888, Percent_Identity=27.1396396396396, Blast_Score=293, Evalue=3e-80,
Organism=Escherichia coli, GI1787172, Length=866, Percent_Identity=28.175519630485, Blast_Score=286, Evalue=4e-78,
Organism=Escherichia coli, GI1789533, Length=828, Percent_Identity=28.743961352657, Blast_Score=281, Evalue=2e-76,
Organism=Escherichia coli, GI1786332, Length=861, Percent_Identity=26.1324041811847, Blast_Score=248, Evalue=1e-66,
Organism=Escherichia coli, GI1786744, Length=880, Percent_Identity=25.2272727272727, Blast_Score=248, Evalue=2e-66,
Organism=Escherichia coli, GI1788427, Length=783, Percent_Identity=26.1813537675607, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI1789610, Length=609, Percent_Identity=23.8095238095238, Blast_Score=120, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000015
- InterPro:   IPR018030 [H]

Pfam domain/function: PF00577 Usher [H]

EC number: NA

Molecular weight: Translated: 97469; Mature: 97469

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS01151 FIMBRIAL_USHER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCMPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFDPRFLELKGDTKIDLGKFSKKGY
CCCCCCCCEEEEEEEEEEEEEEECCCEECCCCCCEEECCEEEEECCCCEEECCCCCCCCC
VDAGKYNLRVFINKQPLSDEYDINWYVSENDPTKTYACLTPELVAALGLKEGIAKSLQWT
EECCCEEEEEEEECCCCCCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCC
HNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSDWDPPSRWDDGIPGLIADYSL
CCCCCCCCCCCCCEECCCCCCCCEEEEEECHHHHHHCCCCCCCCCCCCCCCCCEEEEEEC
NAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDYQHTRSNDDEDDDSSNSTTSK
CCCHHHHHCCCCCCCCCCCCCEECCCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCCC
NWDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIGSSVSTDDQMLPPNLRGYAPDV
CCCHHHEEEECCCCCHHEEEECCHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
SGVAHSSAKVTISQMGRVLYETQVPAGPFRIQDIGDSVSGTLHVRVEEQNGQVQEYDVTT
CCCCCCCCEEEHHHHCCEEEECCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEEEEE
ASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSGGEASWGVADGWSLYGGALADEHYQS
CCCCEEECCCCEEEEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCHHCCCCCCHHHHHH
AAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYGKGKLDGNSFRVSYAKDFDELNSRVTFA
HHHHCCHHHHHHCCEEEEEEECEEECCCCCCCCCCCCCCCEEEEEHHHCHHHHCCEEEEE
GYRFSEKNFMTMSEYLDANQSDMARTGNDKEMYTITYNQNFAAAGVSIYLNYSHRTYWDR
EEEECCCCCEEHHHHHCCCCHHHHHCCCCCEEEEEEECCCEEECCEEEEEEECCCEECCC
PEQTNYNLMFSHYFNMGSIRNMSISVTGYRYEYDDNADKGMYLSMSIPWSDSSTVTYNGS
CCCCCCEEEEEEECCCCCCCEEEEEEEEEEEEECCCCCCCEEEEEECCCCCCCEEEECCC
YGSGSDSSQVGYFKRVDDATHYQVNVGTSEQHGSVDGYLSHDGSLAKVDLSANYHEGEYR
CCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCC
SAGIALQGGATLTAHGGALHRTQSMGGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVA
CCCEEEECCEEEEECCCCEEEHHCCCCEEEEEECCCCEECCCCCCCCEEEEHHCCCHHHH
DINNYYRNQAYIDLNNLPEDAEATQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGS
HHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEEECCC
YPPFGAEVKNDEQQQVGIVDDEGNVYLAGVNAGEHMTVFWEGSAQCEIVLPKPLPADLFS
CCCCCCCCCCCCHHCCCEEECCCCEEEEEECCCCEEEEEECCCCEEEEEECCCCCHHHHC
GLLLPCEQKGTAAPDSSAPEIKPVIQDQTRQVTPTEAPTSISATQ
CCEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCMPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFDPRFLELKGDTKIDLGKFSKKGY
CCCCCCCCEEEEEEEEEEEEEEECCCEECCCCCCEEECCEEEEECCCCEEECCCCCCCCC
VDAGKYNLRVFINKQPLSDEYDINWYVSENDPTKTYACLTPELVAALGLKEGIAKSLQWT
EECCCEEEEEEEECCCCCCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHHCCCC
HNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSDWDPPSRWDDGIPGLIADYSL
CCCCCCCCCCCCCEECCCCCCCCEEEEEECHHHHHHCCCCCCCCCCCCCCCCCEEEEEEC
NAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDYQHTRSNDDEDDDSSNSTTSK
CCCHHHHHCCCCCCCCCCCCCEECCCCCCEEEECCCHHHHHHHCCCCCCCCCCCCCCCCC
NWDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIGSSVSTDDQMLPPNLRGYAPDV
CCCHHHEEEECCCCCHHEEEECCHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
SGVAHSSAKVTISQMGRVLYETQVPAGPFRIQDIGDSVSGTLHVRVEEQNGQVQEYDVTT
CCCCCCCCEEEHHHHCCEEEECCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEEEEE
ASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSGGEASWGVADGWSLYGGALADEHYQS
CCCCEEECCCCEEEEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCHHCCCCCCHHHHHH
AAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYGKGKLDGNSFRVSYAKDFDELNSRVTFA
HHHHCCHHHHHHCCEEEEEEECEEECCCCCCCCCCCCCCCEEEEEHHHCHHHHCCEEEEE
GYRFSEKNFMTMSEYLDANQSDMARTGNDKEMYTITYNQNFAAAGVSIYLNYSHRTYWDR
EEEECCCCCEEHHHHHCCCCHHHHHCCCCCEEEEEEECCCEEECCEEEEEEECCCEECCC
PEQTNYNLMFSHYFNMGSIRNMSISVTGYRYEYDDNADKGMYLSMSIPWSDSSTVTYNGS
CCCCCCEEEEEEECCCCCCCEEEEEEEEEEEEECCCCCCCEEEEEECCCCCCCEEEECCC
YGSGSDSSQVGYFKRVDDATHYQVNVGTSEQHGSVDGYLSHDGSLAKVDLSANYHEGEYR
CCCCCCCCCCCEEEECCCCCEEEEECCCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCC
SAGIALQGGATLTAHGGALHRTQSMGGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVA
CCCEEEECCEEEEECCCCEEEHHCCCCEEEEEECCCCEECCCCCCCCEEEEHHCCCHHHH
DINNYYRNQAYIDLNNLPEDAEATQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGS
HHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEEECCC
YPPFGAEVKNDEQQQVGIVDDEGNVYLAGVNAGEHMTVFWEGSAQCEIVLPKPLPADLFS
CCCCCCCCCCCCHHCCCEEECCCCEEEEEECCCCEEEEEECCCCEEEEEECCCCCHHHHC
GLLLPCEQKGTAAPDSSAPEIKPVIQDQTRQVTPTEAPTSISATQ
CCEEECCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]