Click here to switch to the map view.

The map label for this gene is metB [H]

Identifier: 218700780

GI number: 218700780

Start: 2541383

End: 2542600

Strand: Reverse

Name: metB [H]

Synonym: ECIAI39_2453

Alternate gene names: 218700780

Gene position: 2542600-2541383 (Counterclockwise)

Preceding gene: 218700781

Following gene: 218700779

Centisome position: 49.54

GC content: 44.83

Gene sequence:

>1218_bases
ATGAACAGTTATCAGAACATGTTACTCAATGATGGAAATATTGCCGAATCCGATTACCTTGCCTGTTCCGGTATAAATGA
AAATCATGATGAGTATTTCGGTGCCGTTGCGCCGCCGATTATTCAAACCAGTCTTTTTGTGCAGAAGAATTACCAGCAGT
ATTGTGATGATATGCGTCACGAAGCCCAGCGTTACATATACAGTCGCGGCTTAAATCCAACCGTAAGTATGGTTGAAACC
AAACTTGCTTTGCTGGAGGGGGGTGAAGAGGCGCGGTGCTTTGCATCAGGCATGGGGGCAATCAGCGCAGTGATGGTCGG
TTTGTTAAACAGCGGTGACCATATTTTATTAGTTAATCAAATCTATGGTCCGGCTATCGAATTAGTTAAAAACCTGGCAA
AAAAATACCATATTGAATATGACATTATCTCCAACGGCGATATCAATATTATTGAACAAAAAATCAAACTGAATACCCGA
ATGATATACGCTGAGTCACCCGGCACCATGACAATGCAGATTGTTGATTTACAGGCGATAGCTCAACTGGCGACCCGTTA
TGGGATTATCACCGCAATTGATAACACCTGGCCGACACCGCTATTTCAAAAACCGTTAAGTCTGGGATTTGATATTGTCA
TTCATTCCTGCACAAAATATCTCGCCGGGCACAGTGATGTGTTAGCTGGTGTGGTAATCACCCGCCGTGAATTGATGAGC
ATCATTCGTGATTTCTCGCATCAGCTATTAGGTGCCGTGCTTTCGCCGTTTAGCGCCTGGTTACTGTTACGTGGTATACG
AACACTCCCGATCAGAATGGCAACGCATCAGCATAACGCGATTAACGTTATTGAATGGTTAGCTACTCGTGATGAGGTGG
AAAATATACGTCATCCTCTCTGCGCTAAAGATAAGGAAAAAGAGATAATTCGTCGCCAGATGTGTGGCTTTTCAGGACTG
TTTAGTTTCGAATTAAAAGGAGCCGGCTTTGAGGAAGTGAAATGCTTTATCGATGCCTGCCGTATTTTCAAACCGGGCTG
TAGCTGGGGCGGCTATGAAAGCCTGATTATCTCACCTAACCGTGGTTATAATCAGTCTGCACTGGATGATGCGGAGATCC
CGCGCGGATTAATTCGTCTCTCGCTTGGGCAGGAAAATGCCGCTCTTTTAATTGCCGATCTGGAGCGTGCTTTTGACTCG
CTAAGGACGAAACCATGA

Upstream 100 bases:

>100_bases
TAATTGTCTGCCACGGCAAGTCCGTTACTGGCAAAAGTGCAAAGGTTTTTCCAGATGATAATATTACTATTTCTCTGTAC
AAAAACATAAGGGAAATAAA

Downstream 100 bases:

>100_bases
CAATAAATCAGCCATCCTTTAGCTCGGCGATGATCATTCTGGCTATTATTTTTATATTGTTCCTGGCTGGCGTCGGGATA
CTTAATGCCCCCGTGGAGTT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MNSYQNMLLNDGNIAESDYLACSGINENHDEYFGAVAPPIIQTSLFVQKNYQQYCDDMRHEAQRYIYSRGLNPTVSMVET
KLALLEGGEEARCFASGMGAISAVMVGLLNSGDHILLVNQIYGPAIELVKNLAKKYHIEYDIISNGDINIIEQKIKLNTR
MIYAESPGTMTMQIVDLQAIAQLATRYGIITAIDNTWPTPLFQKPLSLGFDIVIHSCTKYLAGHSDVLAGVVITRRELMS
IIRDFSHQLLGAVLSPFSAWLLLRGIRTLPIRMATHQHNAINVIEWLATRDEVENIRHPLCAKDKEKEIIRRQMCGFSGL
FSFELKGAGFEEVKCFIDACRIFKPGCSWGGYESLIISPNRGYNQSALDDAEIPRGLIRLSLGQENAALLIADLERAFDS
LRTKP

Sequences:

>Translated_405_residues
MNSYQNMLLNDGNIAESDYLACSGINENHDEYFGAVAPPIIQTSLFVQKNYQQYCDDMRHEAQRYIYSRGLNPTVSMVET
KLALLEGGEEARCFASGMGAISAVMVGLLNSGDHILLVNQIYGPAIELVKNLAKKYHIEYDIISNGDINIIEQKIKLNTR
MIYAESPGTMTMQIVDLQAIAQLATRYGIITAIDNTWPTPLFQKPLSLGFDIVIHSCTKYLAGHSDVLAGVVITRRELMS
IIRDFSHQLLGAVLSPFSAWLLLRGIRTLPIRMATHQHNAINVIEWLATRDEVENIRHPLCAKDKEKEIIRRQMCGFSGL
FSFELKGAGFEEVKCFIDACRIFKPGCSWGGYESLIISPNRGYNQSALDDAEIPRGLIRLSLGQENAALLIADLERAFDS
LRTKP
>Mature_405_residues
MNSYQNMLLNDGNIAESDYLACSGINENHDEYFGAVAPPIIQTSLFVQKNYQQYCDDMRHEAQRYIYSRGLNPTVSMVET
KLALLEGGEEARCFASGMGAISAVMVGLLNSGDHILLVNQIYGPAIELVKNLAKKYHIEYDIISNGDINIIEQKIKLNTR
MIYAESPGTMTMQIVDLQAIAQLATRYGIITAIDNTWPTPLFQKPLSLGFDIVIHSCTKYLAGHSDVLAGVVITRRELMS
IIRDFSHQLLGAVLSPFSAWLLLRGIRTLPIRMATHQHNAINVIEWLATRDEVENIRHPLCAKDKEKEIIRRQMCGFSGL
FSFELKGAGFEEVKCFIDACRIFKPGCSWGGYESLIISPNRGYNQSALDDAEIPRGLIRLSLGQENAALLIADLERAFDS
LRTKP

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=354, Percent_Identity=32.4858757062147, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI299473759, Length=354, Percent_Identity=29.3785310734463, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI25453487, Length=353, Percent_Identity=28.8951841359773, Blast_Score=142, Evalue=6e-34,
Organism=Escherichia coli, GI1790375, Length=380, Percent_Identity=35.7894736842105, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1789383, Length=381, Percent_Identity=32.2834645669291, Blast_Score=184, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17560056, Length=363, Percent_Identity=36.9146005509642, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17538055, Length=357, Percent_Identity=36.9747899159664, Blast_Score=228, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI71980708, Length=374, Percent_Identity=31.8181818181818, Blast_Score=192, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6321170, Length=331, Percent_Identity=34.7432024169184, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6319307, Length=397, Percent_Identity=30.2267002518892, Blast_Score=179, Evalue=7e-46,
Organism=Saccharomyces cerevisiae, GI6323333, Length=422, Percent_Identity=27.0142180094787, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6321904, Length=389, Percent_Identity=26.4781491002571, Blast_Score=117, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6321254, Length=395, Percent_Identity=27.0886075949367, Blast_Score=113, Evalue=6e-26,
Organism=Drosophila melanogaster, GI19922554, Length=372, Percent_Identity=36.2903225806452, Blast_Score=231, Evalue=6e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 45266; Mature: 45266

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSYQNMLLNDGNIAESDYLACSGINENHDEYFGAVAPPIIQTSLFVQKNYQQYCDDMRH
CCCHHCCEECCCCCCCCCCEEECCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
EAQRYIYSRGLNPTVSMVETKLALLEGGEEARCFASGMGAISAVMVGLLNSGDHILLVNQ
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
IYGPAIELVKNLAKKYHIEYDIISNGDINIIEQKIKLNTRMIYAESPGTMTMQIVDLQAI
CCCHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHEECCEEEEEECCCCCEEEEEHHHHHH
AQLATRYGIITAIDNTWPTPLFQKPLSLGFDIVIHSCTKYLAGHSDVLAGVVITRRELMS
HHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
IIRDFSHQLLGAVLSPFSAWLLLRGIRTLPIRMATHQHNAINVIEWLATRDEVENIRHPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCC
CAKDKEKEIIRRQMCGFSGLFSFELKGAGFEEVKCFIDACRIFKPGCSWGGYESLIISPN
CCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCC
RGYNQSALDDAEIPRGLIRLSLGQENAALLIADLERAFDSLRTKP
CCCCCCCCCHHHCCCHHEEEECCCCCCEEEHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNSYQNMLLNDGNIAESDYLACSGINENHDEYFGAVAPPIIQTSLFVQKNYQQYCDDMRH
CCCHHCCEECCCCCCCCCCEEECCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
EAQRYIYSRGLNPTVSMVETKLALLEGGEEARCFASGMGAISAVMVGLLNSGDHILLVNQ
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
IYGPAIELVKNLAKKYHIEYDIISNGDINIIEQKIKLNTRMIYAESPGTMTMQIVDLQAI
CCCHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHEECCEEEEEECCCCCEEEEEHHHHHH
AQLATRYGIITAIDNTWPTPLFQKPLSLGFDIVIHSCTKYLAGHSDVLAGVVITRRELMS
HHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
IIRDFSHQLLGAVLSPFSAWLLLRGIRTLPIRMATHQHNAINVIEWLATRDEVENIRHPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCC
CAKDKEKEIIRRQMCGFSGLFSFELKGAGFEEVKCFIDACRIFKPGCSWGGYESLIISPN
CCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCC
RGYNQSALDDAEIPRGLIRLSLGQENAALLIADLERAFDSLRTKP
CCCCCCCCCHHHCCCHHEEEECCCCCCEEEHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]