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The map label for this gene is menE

Identifier: 218700735

GI number: 218700735

Start: 2492662

End: 2494017

Strand: Reverse

Name: menE

Synonym: ECIAI39_2408

Alternate gene names: 218700735

Gene position: 2494017-2492662 (Counterclockwise)

Preceding gene: 218700736

Following gene: 218700734

Centisome position: 48.6

GC content: 56.64

Gene sequence:

>1356_bases
ATGATCTTCTCTGACTGGCCGTGGCGTCACTGGCGGCAAGTGCGGGGAGAAGCCATCGCCTTACGTCTCAATGACGAGCA
ACTCAACTGGCGCGAGCTTTGTGCTCGCGTCGATGAATTAGCCTCCAGCTTTGCGGTGCAGGGTGTGGTTGAGGGCAGCG
GCGTGATGTTGCGGGCGTGGAATACGCCGCAAACGCTGCTGGCCTGGCTGGCGTTACTGCAATGCGGGGCGCGGGTGTTG
CCCGTGAACCCTCAGCTGCCGCAACCGTTGCTTGAAGAATTGCTGCCCAATCTGACGCTGCAATTTGCGCTGGTGCCGGA
AGGGGAAAACACGTTTCCGGCGTTAACGTCGCTGCACATTCAGCTGGTTGAAGGCGCACATGCCGCTGCGTGGCAGCCGA
CGCGTCTGTGCTCAATGACGTTAACCTCTGGTTCCACTGGCTTGCCGAAAGCCGCTGTTCATACTTATCAGGCTCATCTT
GCCAGCGCCGAAGGGGTGTTATCGCTGATTCCGTTTGGCGATCACGATGACTGGTTGCTCTCTTTACCGCTGTTTCACGT
CTCCGGTCAGGGAATTATGTGGCGCTGGTTATACGCTGGTGCGCGGATGACGGTACGTGATAAACAGCCATTGGAGCAAA
TGCTGGCAGGCTGTACTCACGCTTCACTGGTGCCAACACAACTCTGGCGTTTGCTGGTTAACCGTAGTTCCGTTTCCCTG
AAAGCGGTTTTACTTGGCGGCGCGGCTATCCCGGTCGAGTTGACGGAACAGGCGCGCGAGCAGGGGATTCGTTGCTTTTG
CGGCTATGGTCTGACCGAGTTTGCCTCCACGGTGTGTGCGAAAGAAGCCGACGGCCTGGCGGACGTTGGTTCGCCGCTGC
CGGGTCGGGAAGTGAAAATCGTTAATGATGAAGTGTGGCTGCGGGCTGCCAGTATGGCAGAAGGTTACTGGCGTAACGGG
CAACGGGTTCCACTGGTTAATGATGAAGGCTGGTACGCCACGCGCGATCGCGGTGAGATGCATAATGGCAAGCTGACCAT
TGTCGGACGTTTAGACAATCTATTCTTCAGTGGCGGTGAGGGTATTCAGCCGGAAGAAGTCGAGCGCGTTATTGCTGCAC
ATCCTGCGGTTTTGCAGGTGTTTATCGTTCCCGTTGCCGACAAGGAGTTTGGTCATCGGCCGGTAGCGGTGGTGGAGTAT
GACCAGCAAAGCGTTGATCTTGACGAATGGGTGAAAGATAAGCTGGCTCGTTTTCAACAACCGGTGCGCTGGCTGACTCT
GCCGCCGGAGCTGAAAAACGGCGGTATTAAAATTTCACGTCAGGCGCTAAAAGAGTGGGTGCAACGCCAGCAATAA

Upstream 100 bases:

>100_bases
CGGGACTGGACACGCTGGATCTGATGCAGGTGCAGCAGGTTCGTCGCTGGCCGGGGAGCCCACTGCCGTTAGTGGACGTT
GATGCACTGGAGCGGCTGTT

Downstream 100 bases:

>100_bases
TTAACCCTCCGTTTCACTTCTCATTTCTGCCATTCCATTGCCGTTGATAAAGAGTTTATCTGTTAATCTGTAAATTGATG
TGAAAACTCTTAGCAAACAG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSFAVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPEGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNG
QRVPLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEY
DQQSVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ

Sequences:

>Translated_451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSFAVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPEGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNG
QRVPLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEY
DQQSVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ
>Mature_451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSFAVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPEGENTFPALTSLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNG
QRVPLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEY
DQQSVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=542, Percent_Identity=22.140221402214, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788595, Length=451, Percent_Identity=97.3392461197339, Blast_Score=857, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=350, Percent_Identity=25.4285714285714, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI221142682, Length=494, Percent_Identity=23.2793522267206, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786810, Length=507, Percent_Identity=23.4714003944773, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788107, Length=352, Percent_Identity=26.7045454545455, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789201, Length=364, Percent_Identity=25, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI32563687, Length=465, Percent_Identity=25.3763440860215, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17558820, Length=474, Percent_Identity=25.5274261603376, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=499, Percent_Identity=24.8496993987976, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994694, Length=342, Percent_Identity=27.1929824561404, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71994690, Length=342, Percent_Identity=27.1929824561404, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71994703, Length=342, Percent_Identity=27.1929824561404, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=495, Percent_Identity=22.6262626262626, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=295, Percent_Identity=26.1016949152542, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17538037, Length=498, Percent_Identity=21.6867469879518, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25141359, Length=400, Percent_Identity=23.5, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71996755, Length=343, Percent_Identity=23.6151603498542, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24581924, Length=531, Percent_Identity=22.4105461393597, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 50210; Mature: 50210

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSFAVQGVVEGSGVMLRAW
CCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEC
NTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQFALVPEGENTFPALTSLHI
CCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHEE
QLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAEGVLSLIPFGDHDDWLL
EEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEE
SLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
EEEEEEECCCCEEEEEEECCCEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEE
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKI
EEEEECCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCEEEE
VNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGE
ECHHHHEEHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCEEEEEEEECCCEECCCC
GIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEYDQQSVDLDEWVKDKLARFQQ
CCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHC
PVRWLTLPPELKNGGIKISRQALKEWVQRQQ
CCEEEECCHHHCCCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSFAVQGVVEGSGVMLRAW
CCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEECCCCEEEEEC
NTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQFALVPEGENTFPALTSLHI
CCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHEE
QLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAEGVLSLIPFGDHDDWLL
EEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEE
SLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
EEEEEEECCCCEEEEEEECCCEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEE
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKI
EEEEECCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCHHCCCCCCCCEEEE
VNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGE
ECHHHHEEHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCCCEEEEEEEECCCEECCCC
GIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVVEYDQQSVDLDEWVKDKLARFQQ
CCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHC
PVRWLTLPPELKNGGIKISRQALKEWVQRQQ
CCEEEECCHHHCCCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626063; 9205837; 9278503 [H]