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The map label for this gene is ais

Identifier: 218700726

GI number: 218700726

Start: 2484032

End: 2484634

Strand: Reverse

Name: ais

Synonym: ECIAI39_2399

Alternate gene names: 218700726

Gene position: 2484634-2484032 (Counterclockwise)

Preceding gene: 218700734

Following gene: 218700724

Centisome position: 48.41

GC content: 43.12

Gene sequence:

>603_bases
GTGTTAGCTTTTTGCCGCTCTTCGTTGAAGTCAAAAAAATATTTCATCATTTTACTGGCGCTCGCTGCAATTGCCGGACT
GGGTACTCATGCCGCCTGGAGTAGCAATGGTTTGCCACGTATCGACAATAAAACGCTGGCCAGACTGGCACAGCAGCATC
CGGTTGTCGTTTTGTTTCGCCATGCTGAACGTTGCGATCGTTCAACCAATCAATGCTTGTCAGATAAAACAGGTATTACG
GTCAAAGGTACCCAGGATGCCCGTGAACTGGGCAACGCTTTTAGTGCTGATATCCCTGATTTCGATCTTTATTCCAGTAA
TACCGTCCGGACCATTCAGTCGGCGACCTGGTTTTCAGCGGGTAAAAAGCTGACGGTAGATAAACGACTTCTTCAATGCG
GTAATGAGATTTATAGTGCAATTAAGAACTTACAAAGCAAAGCACCTGATAAAAATATCGTTATTTTTACCCATAATCAT
TGCCTGACATATATTGCTAAAGATAAGCGTGATGCGACATTTAAACCTGATTATCTGGATGGTTTAGTCATGCATGTGGA
AAAAGGCAAAGTTTATCTGGATGGGGAATTCGTTAACCACTAA

Upstream 100 bases:

>100_bases
GTAGGATTTTTAATATTACACCAACTGCTAATTATCGCCAGAAATGTCGCTCAATTTCACGGTAATTGTCTGGTTGCACT
TGTCTATAGGTGGAGTTTAC

Downstream 100 bases:

>100_bases
ATTTTCAATCTGACAGCCAGTAATGGCTGTCATCATTGTTACAGAAGACCTTTCAAACGTAACGTTTTTCGGGTGGCGAC
ATTCAAATCATAGTGCGCCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNKTLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGIT
VKGTQDARELGNAFSADIPDFDLYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKNLQSKAPDKNIVIFTHNH
CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH

Sequences:

>Translated_200_residues
MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNKTLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGIT
VKGTQDARELGNAFSADIPDFDLYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKNLQSKAPDKNIVIFTHNH
CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH
>Mature_200_residues
MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNKTLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGIT
VKGTQDARELGNAFSADIPDFDLYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKNLQSKAPDKNIVIFTHNH
CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH

Specific function: Catalyzes the dephosphorylation of heptose(II) of the outer membrane lipopolysaccharide core [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate mutase family. Ais subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788586, Length=200, Percent_Identity=99, Blast_Score=413, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013078
- InterPro:   IPR011310 [H]

Pfam domain/function: PF00300 PGAM [H]

EC number: NA

Molecular weight: Translated: 22290; Mature: 22290

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNKTLARLAQQHPVVVLFR
CCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEE
HAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFDLYSSNTVRTIQSATWFSA
CHHHHCHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHC
GKKLTVDKRLLQCGNEIYSAIKNLQSKAPDKNIVIFTHNHCLTYIAKDKRDATFKPDYLD
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCHHHH
GLVMHVEKGKVYLDGEFVNH
HEEEEEECCEEEEECEECCC
>Mature Secondary Structure
MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNKTLARLAQQHPVVVLFR
CCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEE
HAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFDLYSSNTVRTIQSATWFSA
CHHHHCHHHHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHC
GKKLTVDKRLLQCGNEIYSAIKNLQSKAPDKNIVIFTHNHCLTYIAKDKRDATFKPDYLD
CCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCCCCCCCCHHHH
GLVMHVEKGKVYLDGEFVNH
HEEEEEECCEEEEECEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA