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The map label for this gene is dgoD [H]

Identifier: 218700699

GI number: 218700699

Start: 2451862

End: 2453076

Strand: Reverse

Name: dgoD [H]

Synonym: ECIAI39_2370

Alternate gene names: 218700699

Gene position: 2453076-2451862 (Counterclockwise)

Preceding gene: 218700700

Following gene: 218700698

Centisome position: 47.8

GC content: 40.66

Gene sequence:

>1215_bases
ATGAGGTTATTTATGAAGATTACATCTGTAGAAATTTTTGACTGCGAACTCAAAAAAAGAGATCAAACTATGGCCTCTTA
TAATCCAGTACTTATTCGGGTTAATACCGATGCTGGAATAAGTGGTGTTGGCGAAGTAGGATTAGCCTATGGTGCAGGTG
CAAAATCTGGTGTTGGTATTATCAGAGATTTAGCCCCTCTCATCATTGGTGAAGATCCGTTGAACATTGAGAAGATTTGG
GAATTCCTCTTCAGGAAAACATTTTGGGGAATGGGCGGTGGTAATGTTTTTTACGCAGGTATGAGCGCTATTGATATTGC
CTTATGGGATATTAAAGGTAAATATTTAAACGTGCCGGTTTATCAACTACTGGGGGGAAAGACTAACGATAAATTGAGAA
CTTACGCCAGTCAGTTACAATTTGGTTGGGGCGAAAAAAGGCAAATATTAGTAACGCCTGAAGAGTATGCAGAGGCAGCG
CGTGCCGCTATTGCAGAAGGTTATAATGCGATAAAAGTTGATCCGCTTGAAATCGATCTGCATGGCGGCGATTGTGTCTT
TCAGAACAAAAATAGAAATTACTCTGGCTTATTACTGGCCGAACAATTAAAAATGGGCGAGGCAAGAATTGCCGCTATGC
GTGAAGCAATGGGCGACGATGCTGATATTATTGTAGAGATTCATTCTCTTCTAGGAACAAATTCAGCGATCCAGTTTGCC
AAAGCAATAGAGAAATATCGTATTTTTCTCTATGAGGAGCCAATTCATCCATTAAATTCGGATAATATGCAGAAAGTTGC
CCGTTCAACATCGATTCCGATAGCAACAGGTGAGCGTTCTTATACTCGCTGGGGATATCGTGATTTACTGGAAAAACAAT
CAATTGCGGTAGCTCAACCTGATTTGTGTCTTTGTGGTGGCATTACAGAGGGAAAGAAAATTTGTGATTATGCCAATATA
TATGACACAACCGTTCAGGTACATGTTTGTGGCGGGCCTGTTTCAACAGTAGCGGCGCTGCATATGGAAACTGCAATACC
TAACTTTATTATTCATGAACATCATACCAATGCGATGAAAGCATGTATTCGGGAACTTTGTACTTACGATTATCAACCCG
AGAATGGCTATTATGTCGCGCCAGAGTTGCCTGGGCTTGGTCAGGAATTAAACGATGAAATCGTACAACAATATCTTGCC
TATGTGATCAAATAA

Upstream 100 bases:

>100_bases
GATTCTTTGCTTTATCGCCGTAGCTATTTTGTTATCAATGAAAATTGATAATAAAAAAGATAACGCCCAGAAAGACAATG
TTATTTCACATGCTAAATAA

Downstream 100 bases:

>100_bases
CGGCGATTTATATACTCTAAATAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGAATCCCCAGGAGCTTACATAAGTAAG
TGACTGGGGTGAGCGAACGC

Product: Mandelate racemase/muconate lactonizing enzyme family protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MRLFMKITSVEIFDCELKKRDQTMASYNPVLIRVNTDAGISGVGEVGLAYGAGAKSGVGIIRDLAPLIIGEDPLNIEKIW
EFLFRKTFWGMGGGNVFYAGMSAIDIALWDIKGKYLNVPVYQLLGGKTNDKLRTYASQLQFGWGEKRQILVTPEEYAEAA
RAAIAEGYNAIKVDPLEIDLHGGDCVFQNKNRNYSGLLLAEQLKMGEARIAAMREAMGDDADIIVEIHSLLGTNSAIQFA
KAIEKYRIFLYEEPIHPLNSDNMQKVARSTSIPIATGERSYTRWGYRDLLEKQSIAVAQPDLCLCGGITEGKKICDYANI
YDTTVQVHVCGGPVSTVAALHMETAIPNFIIHEHHTNAMKACIRELCTYDYQPENGYYVAPELPGLGQELNDEIVQQYLA
YVIK

Sequences:

>Translated_404_residues
MRLFMKITSVEIFDCELKKRDQTMASYNPVLIRVNTDAGISGVGEVGLAYGAGAKSGVGIIRDLAPLIIGEDPLNIEKIW
EFLFRKTFWGMGGGNVFYAGMSAIDIALWDIKGKYLNVPVYQLLGGKTNDKLRTYASQLQFGWGEKRQILVTPEEYAEAA
RAAIAEGYNAIKVDPLEIDLHGGDCVFQNKNRNYSGLLLAEQLKMGEARIAAMREAMGDDADIIVEIHSLLGTNSAIQFA
KAIEKYRIFLYEEPIHPLNSDNMQKVARSTSIPIATGERSYTRWGYRDLLEKQSIAVAQPDLCLCGGITEGKKICDYANI
YDTTVQVHVCGGPVSTVAALHMETAIPNFIIHEHHTNAMKACIRELCTYDYQPENGYYVAPELPGLGQELNDEIVQQYLA
YVIK
>Mature_404_residues
MRLFMKITSVEIFDCELKKRDQTMASYNPVLIRVNTDAGISGVGEVGLAYGAGAKSGVGIIRDLAPLIIGEDPLNIEKIW
EFLFRKTFWGMGGGNVFYAGMSAIDIALWDIKGKYLNVPVYQLLGGKTNDKLRTYASQLQFGWGEKRQILVTPEEYAEAA
RAAIAEGYNAIKVDPLEIDLHGGDCVFQNKNRNYSGLLLAEQLKMGEARIAAMREAMGDDADIIVEIHSLLGTNSAIQFA
KAIEKYRIFLYEEPIHPLNSDNMQKVARSTSIPIATGERSYTRWGYRDLLEKQSIAVAQPDLCLCGGITEGKKICDYANI
YDTTVQVHVCGGPVSTVAALHMETAIPNFIIHEHHTNAMKACIRELCTYDYQPENGYYVAPELPGLGQELNDEIVQQYLA
YVIK

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=375, Percent_Identity=28.5333333333333, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1787864, Length=407, Percent_Identity=28.992628992629, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI226510960, Length=362, Percent_Identity=25.414364640884, Blast_Score=88, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 44866; Mature: 44866

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFMKITSVEIFDCELKKRDQTMASYNPVLIRVNTDAGISGVGEVGLAYGAGAKSGVGI
CEEEEEEEEEEEEECHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHEECCCCCCCCHH
IRDLAPLIIGEDPLNIEKIWEFLFRKTFWGMGGGNVFYAGMSAIDIALWDIKGKYLNVPV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEEEECCCEECCCH
YQLLGGKTNDKLRTYASQLQFGWGEKRQILVTPEEYAEAARAAIAEGYNAIKVDPLEIDL
HHHHCCCCCHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEECEEEEEE
HGGDCVFQNKNRNYSGLLLAEQLKMGEARIAAMREAMGDDADIIVEIHSLLGTNSAIQFA
CCCEEEEECCCCCCCEEEEHHHHHCCHHHHHHHHHHHCCCCHHHHEEHHHHCCCHHHHHH
KAIEKYRIFLYEEPIHPLNSDNMQKVARSTSIPIATGERSYTRWGYRDLLEKQSIAVAQP
HHHHHHEEEEEECCCCCCCCCHHHHHHHHCCCCEECCCCCHHHCCHHHHHHHCCCEECCC
DLCLCGGITEGKKICDYANIYDTTVQVHVCGGPVSTVAALHMETAIPNFIIHEHHTNAMK
CEEEECCCCCCHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCHHHH
ACIRELCTYDYQPENGYYVAPELPGLGQELNDEIVQQYLAYVIK
HHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRLFMKITSVEIFDCELKKRDQTMASYNPVLIRVNTDAGISGVGEVGLAYGAGAKSGVGI
CEEEEEEEEEEEEECHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHEECCCCCCCCHH
IRDLAPLIIGEDPLNIEKIWEFLFRKTFWGMGGGNVFYAGMSAIDIALWDIKGKYLNVPV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEEEECCCEECCCH
YQLLGGKTNDKLRTYASQLQFGWGEKRQILVTPEEYAEAARAAIAEGYNAIKVDPLEIDL
HHHHCCCCCHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEECEEEEEE
HGGDCVFQNKNRNYSGLLLAEQLKMGEARIAAMREAMGDDADIIVEIHSLLGTNSAIQFA
CCCEEEEECCCCCCCEEEEHHHHHCCHHHHHHHHHHHCCCCHHHHEEHHHHCCCHHHHHH
KAIEKYRIFLYEEPIHPLNSDNMQKVARSTSIPIATGERSYTRWGYRDLLEKQSIAVAQP
HHHHHHEEEEEECCCCCCCCCHHHHHHHHCCCCEECCCCCHHHCCHHHHHHHCCCEECCC
DLCLCGGITEGKKICDYANIYDTTVQVHVCGGPVSTVAALHMETAIPNFIIHEHHTNAMK
CEEEECCCCCCHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCHHHH
ACIRELCTYDYQPENGYYVAPELPGLGQELNDEIVQQYLAYVIK
HHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA