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The map label for this gene is alkB

Identifier: 218700680

GI number: 218700680

Start: 2420905

End: 2421555

Strand: Reverse

Name: alkB

Synonym: ECIAI39_2349

Alternate gene names: 218700680

Gene position: 2421555-2420905 (Counterclockwise)

Preceding gene: 218700681

Following gene: 218700679

Centisome position: 47.18

GC content: 53.46

Gene sequence:

>651_bases
ATGTTGGATCTGTTTGCCGATGCTGAACCGTGGCAAGAACCACTGGCGGCTGGAGCGGTGATTTTGCACCGTTTTGCTTT
TAACGCTGCGGAGCAACTGATCCGCGATATTAATGACGTTGCCAGCCAGTCGCCGTTTCGCCAGATGGTCACTCCAGGCG
GATATACCATGTCGGTGGCGATGACCAACTGTGGGCATCTGGGCTGGACGACCCATCGGCAAGGTTATCTCTATTCGCCC
ATTGATCCGCAAACAAATAAACCGTGGCCCGCCATGCCGCAGAGTTTTCATGATTTATGCCAACGTGCGGCTACGGCGGC
GGGCTATCCAGATTTCCAGCCAGATGCCTGTTTGATCAACCGCTACGTTCCTGGCGCGAAACTGTCGCTGCATCAGGATA
AAGACGAACCGGATCTGCGCGCGCCAATTGTTTCTGTTTCTCTGGGCTTACCCGCGATTTTTCAATTTGGCGGCCTGAAA
CGAAATGATCCGCTCAAACGTTTGTTGTTGGAACATGGCGATGTGGTGGTATGGGGTGGTGAGTCGCGGCTGTTTTATCA
TGGCATTCAACCGTTGAAAGTGGGGGGGCATCCACTCACCACCGACTGCCGCTACAACCTGACATTCCGGCAGGCAGGTA
AAAAAGAATAA

Upstream 100 bases:

>100_bases
ATCGGGTGGTCCGTGGTGATGGCACACTTTCCGGTTACCGCTGGGGCGTGTCGCGCAAAGCGCAACTGCTGCGCCGCGAA
GCTGAAAATGAGGAAAGGTA

Downstream 100 bases:

>100_bases
AAATAAGAATTATTATTGCTGTGCGCGCGAAGATGTTTAAACTGCGGGCTGTAATTCATTGTCCGGGTTTTCTGCATGGA
ACTTCTTGTACTTGTCTGGC

Product: N1-methyladenine or N3-methylcytosine DNA lesion oxidative demethylase

Products: NA

Alternate protein names: Alkylated DNA repair protein AlkB [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQSPFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSP
IDPQTNKPWPAMPQSFHDLCQRAATAAGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKVGGHPLTTDCRYNLTFRQAGKKE

Sequences:

>Translated_216_residues
MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQSPFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSP
IDPQTNKPWPAMPQSFHDLCQRAATAAGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKVGGHPLTTDCRYNLTFRQAGKKE
>Mature_216_residues
MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQSPFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSP
IDPQTNKPWPAMPQSFHDLCQRAATAAGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKVGGHPLTTDCRYNLTFRQAGKKE

Specific function: Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable a

COG id: COG3145

COG function: function code L; Alkylated DNA repair protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Homo sapiens, GI87298840, Length=98, Percent_Identity=33.6734693877551, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1788541, Length=216, Percent_Identity=97.2222222222222, Blast_Score=439, Evalue=1e-125,
Organism=Drosophila melanogaster, GI45555401, Length=125, Percent_Identity=36.8, Blast_Score=84, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004574 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24012; Mature: 24012

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQSPFRQMVTPGGYTMSVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEE
MTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQRAATAAGYPDFQPDACLIN
ECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHC
RYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLKRNDPLKRLLLEHGDVVVWGG
CCCCCCEEEECCCCCCCCHHCCEEEEECCCHHHHHHCCCCCCCHHHHHHHHCCCEEEECC
ESRLFYHGIQPLKVGGHPLTTDCRYNLTFRQAGKKE
CCEEEEECCCEEEECCCCCCCCCEEEEEHHCCCCCC
>Mature Secondary Structure
MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQSPFRQMVTPGGYTMSVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEE
MTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQRAATAAGYPDFQPDACLIN
ECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHC
RYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLKRNDPLKRLLLEHGDVVVWGG
CCCCCCEEEECCCCCCCCHHCCEEEEECCCHHHHHHCCCCCCCHHHHHHHHCCCEEEECC
ESRLFYHGIQPLKVGGHPLTTDCRYNLTFRQAGKKE
CCEEEEECCCEEEECCCCCCCCCEEEEEHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536913; 9097040; 9278503; 3887409; 7928996 [H]