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The map label for this gene is napH

Identifier: 218700673

GI number: 218700673

Start: 2411494

End: 2412357

Strand: Reverse

Name: napH

Synonym: ECIAI39_2342

Alternate gene names: 218700673

Gene position: 2412357-2411494 (Counterclockwise)

Preceding gene: 218700674

Following gene: 218700672

Centisome position: 47.01

GC content: 56.25

Gene sequence:

>864_bases
ATGGCAAATCGTAAACGTGACGCCGGGCGCGAGGCGCTGGAGAAAAAAGGCTGGTGGCGCAGCCACCGTTGGCTGGTGTT
ACGTCGCCTTTGTCAGTTCTTCGTGCTGGGGATGTTTTTGAGCGGTCCGTGGTTTGGTGTGTGGATCTTGCACGGCAACT
ACAGCAGTAGCCTGTTATTCGACACCGTTCCGCTGACCGAGCCGCTGATGACGCTGCAAAGTCTTGCCAGCGGTCATCTG
CCTGCCACGGTGGCGTTGACCGGTGCGGTAATTATCACCGTGCTCTACGCCCTGGCAGGAAAGCGATTATTTTGCAGCTG
GGTCTGCCCGCTGAACCCGATTACCGACTTAGCGAACTGGTTACGCAGGCGCTTTGACCTCAATCAGTCTGCGACGATCC
CTCGCCACATTCGCTACGTGCTGTTGGTGGTCATTCTGCTGGGATCGGCCTTAACCGGCACGCTCATCTGGGAATGGATC
AACCCCGTTTCCCTGATGGGGCGCAGTCTGGTAATGGGATTCGGCAGCGGCGCGCTGCTTATTCTCGCGCTGTTTTTATT
TGATCTACTGGTCGTTGAACACGGCTGGTGCGGGCACATTTGTCCGGTTGGCGCGCTGTATGGCGTGCTGGGTAGCAAAG
GTGTGATTACCGTTGCTGCCAACGATCGGCAGAAATGTAACCGCTGTATGGATTGTTTTCATGTTTGCCCGGAACCGCAT
GTGCTACGTGCCCCGGTGCTGGATGAACAAAGCCCGGTGCAGGTCACCAGCCGCGATTGCATGACGTGCGGTCGCTGCGT
GGATGTCTGTTCTGAGGATGTATTTACAATAACTACACGATGGAGTTCGGGAGCGAAATCATGA

Upstream 100 bases:

>100_bases
GTGTGCGTGCTGGAACAACCGGCAATCAAGGTGTTACCGCTGTCACTGGCGAAAGGGGAGTTAGGTCACCATTACCGCTT
CGGCTGGCTGGAGGGGAACA

Downstream 100 bases:

>100_bases
AAAGCCATGACCTGAAGAAAGCGCTGTGTCAATGGACGGCGATGCTGGCCCTGGTGGTAAGCGGCGCGGTTTGGGCGGCT
AATGGCGTCGATTTTAGCCA

Product: quinol dehydrogenase membrane component

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPWFGVWILHGNYSSSLLFDTVPLTEPLMTLQSLASGHL
PATVALTGAVIITVLYALAGKRLFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITVAANDRQKCNRCMDCFHVCPEPH
VLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFTITTRWSSGAKS

Sequences:

>Translated_287_residues
MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPWFGVWILHGNYSSSLLFDTVPLTEPLMTLQSLASGHL
PATVALTGAVIITVLYALAGKRLFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITVAANDRQKCNRCMDCFHVCPEPH
VLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFTITTRWSSGAKS
>Mature_286_residues
ANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPWFGVWILHGNYSSSLLFDTVPLTEPLMTLQSLASGHLP
ATVALTGAVIITVLYALAGKRLFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWIN
PVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITVAANDRQKCNRCMDCFHVCPEPHV
LRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFTITTRWSSGAKS

Specific function: Involved in electron transfer [H]

COG id: COG0348

COG function: function code C; Polyferredoxin

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788532, Length=287, Percent_Identity=98.9547038327526, Blast_Score=579, Evalue=1e-167,
Organism=Escherichia coli, GI1787226, Length=265, Percent_Identity=27.5471698113208, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR011886 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 31915; Mature: 31784

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPWFGVWILHGNYSSSLLF
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEE
DTVPLTEPLMTLQSLASGHLPATVALTGAVIITVLYALAGKRLFCSWVCPLNPITDLANW
ECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
LRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWINPVSLMGRSLVMGFGSGALL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHH
ILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITVAANDRQKCNRCMDCFHVCPEPH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCC
VLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFTITTRWSSGAKS
EEECCCCCCCCCCEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
ANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPWFGVWILHGNYSSSLLF
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEE
DTVPLTEPLMTLQSLASGHLPATVALTGAVIITVLYALAGKRLFCSWVCPLNPITDLANW
ECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
LRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWINPVSLMGRSLVMGFGSGALL
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHH
ILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITVAANDRQKCNRCMDCFHVCPEPH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCC
VLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFTITTRWSSGAKS
EEECCCCCCCCCCEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]