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The map label for this gene is galS

Identifier: 218700623

GI number: 218700623

Start: 2354856

End: 2355896

Strand: Reverse

Name: galS

Synonym: ECIAI39_2290

Alternate gene names: 218700623

Gene position: 2355896-2354856 (Counterclockwise)

Preceding gene: 218700624

Following gene: 218700622

Centisome position: 45.91

GC content: 51.78

Gene sequence:

>1041_bases
ATGATCACCATTCGTGATGTAGCGCGTCAGGCTGGCGTCTCTGTGGCAACGGTTTCCCGGGTGCTCAATAACAGCACGCT
GGTCAGTGCCGACACGCGTGAAGCAGTAATGAAAGCCGTGAGTGAGCTGGATTATCGACCAAACGCCAATGCCCAGGCGC
TGGCAACTCAGGTTAGCGATACCATTGGCGTGGTGGTGATGGACGTTTCTGATGCGTTTTTTGGTGCGCTGGTAAAAGCG
GTGGATTTGGTCGCCCAGCAGCATCAGAAATACGTGCTAATAGGCAATAGCTATCATGAAGCGGAAAAAGAGCGTCACGC
CATTGAGGTGTTAATTCGCCAGCGTTGTAATGCGTTGATTGTTCACTCAAAAGCATTGAGTGACGATGAACTGGCACAAT
TTATGGATAACATTCCCGGTATGGTGTTAATCAACCGCGTTGTGCCGGGGTACGCCCATCGTTGCGTTTGCCTGGATAAT
CTCAGTGGTGCCCGAATGGCGACGCGCATGTTGCTGAATAACGGCCATCAACGTATTGGTTATCTTTCTTCCAGCCACGG
CATTGAAGATGACGCCATGCGTAAAGCAGGCTGGATGAGTGCGTTGAAAGAGCAGGATATTATTCCGCCGGAAAGCTGGA
TTGGCACTGGCACGCCTGACATGCCGGGCGGTGAGGCGGCGATGGTTGAACTGCTGGGGCGCAATCTACAACTTACCGCT
GTATTTGCTTATAACGACAATATGGCTGCTGGCGCACTGACAGCATTAAAAGATAATGGCATTGCGATTCCGTTACATCT
CTCCATCATCGGTTTCGATGATATTCCCATCGCCCGTTACACCGACCCGCAATTAACGACCGTGCGTTATCCCATTGCTT
CAATGGCGAAATTAGCCACCGAACTGGCCTTGCTGGGGGCAGCAGGCAATATTGATCCTCGTGCCAGCCACTGTTTTATG
CCGACGTTAGTGCGTCGCCATTCTGTCGCAACACGCCAGAATGCGGCGGCGATCACTAACTCAACAAATCAGGCGATGTA
A

Upstream 100 bases:

>100_bases
TCCATTGCTGTGACTCGATTCACGAAGTCCTGTATTCAGTGCTGACAAAATAGCCGCCAGCAAGCAGTCATTTACTGCAA
TCTCATAACAGGTAGTGAAT

Downstream 100 bases:

>100_bases
CCGCTTTCAATCTGTGAGTGATTTCACAGTATCTTAACAATGTGATAGCTATGATTGCACCGTTTTAACGTTGTAACCCG
TATGTAACAGTGAATAATCA

Product: DNA-binding transcriptional regulator GalS

Products: NA

Alternate protein names: Mgl repressor and galactose ultrainduction factor [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELLGRNLQLTA
VFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLATELALLGAAGNIDPRASHCFM
PTLVRRHSVATRQNAAAITNSTNQAM

Sequences:

>Translated_346_residues
MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELLGRNLQLTA
VFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLATELALLGAAGNIDPRASHCFM
PTLVRRHSVATRQNAAAITNSTNQAM
>Mature_346_residues
MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELLGRNLQLTA
VFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLATELALLGAAGNIDPRASHCFM
PTLVRRHSVATRQNAAAITNSTNQAM

Specific function: Repressor of the mgl operon. Binds galactose and D- fucose as inducers. GalS binds to an operator DNA sequence within its own coding sequence (corresponding to residues 15 to 20) [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788474, Length=346, Percent_Identity=99.4219653179191, Blast_Score=707, Evalue=0.0,
Organism=Escherichia coli, GI1789202, Length=331, Percent_Identity=55.8912386706949, Blast_Score=354, Evalue=6e-99,
Organism=Escherichia coli, GI1789068, Length=334, Percent_Identity=35.6287425149701, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1790369, Length=302, Percent_Identity=34.7682119205298, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=33.6336336336336, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1790194, Length=305, Percent_Identity=29.8360655737705, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=28.75, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786540, Length=298, Percent_Identity=31.2080536912752, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787906, Length=261, Percent_Identity=29.8850574712644, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI48994940, Length=307, Percent_Identity=25.7328990228013, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790715, Length=312, Percent_Identity=25.9615384615385, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789456, Length=318, Percent_Identity=27.0440251572327, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790689, Length=291, Percent_Identity=25.7731958762887, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786268, Length=207, Percent_Identity=26.5700483091787, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37372; Mature: 37372

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSD
CEEHHHHHHHHCCHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALI
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCEEE
VHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNNGHQRIG
EECCCCCHHHHHHHHHCCCCEEEHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHEE
YLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELLGRNLQLTA
EECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHCCCEEEEE
VFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLAT
EEEECCCCCHHHHHEECCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHH
ELALLGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEECCCCCCC
>Mature Secondary Structure
MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQVSD
CEEHHHHHHHHCCHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCNALI
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCEEE
VHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNNGHQRIG
EECCCCCHHHHHHHHHCCCCEEEHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCCCHHEE
YLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELLGRNLQLTA
EECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCCHHHHHHHHCCCEEEEE
VFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRYPIASMAKLAT
EEEECCCCCHHHHHEECCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHH
ELALLGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1619663; 9278503; 8208287; 8982002 [H]