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The map label for this gene is ycbF

Identifier: 218700537

GI number: 218700537

Start: 2249717

End: 2250427

Strand: Reverse

Name: ycbF

Synonym: ECIAI39_2203

Alternate gene names: 218700537

Gene position: 2250427-2249717 (Counterclockwise)

Preceding gene: 218700538

Following gene: 218700536

Centisome position: 43.85

GC content: 50.91

Gene sequence:

>711_bases
ATGACTAATACGTGGAACAGATTGGCGCTCTTGATTTTCGCCGTTTTATCGCTGCTGGTGGCGAGTGAATTGCAGGCAGG
AGTGGTGGTCGGCGGAACGCGATTTATCTTTCCGGCAGACAGAGATTCGATATCTATTTCACTGACTAATACCAGTCAGG
AATCCTGGCTTATTAACAGTAAAATCAATCGCCCAACGCGTTGGGCTGGGGGGGAAGCGTCGACAGTACCAGCACCATTA
CTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACTACAGGCACATTGCGCTTGCTGAGAACGGAAAGCGACATCTT
GCCCGTGGATCGCGAAACGCTATTTGAGTTAAGCATTGCCAGCGTGCCATCCGGCAAAGTTGAAAATCAGAGCGTAAAAG
TGGCGATGCGCTCGGTATTTAAACTGTTCTGGCGACCCGAAGGTTTGCCGGGCGACCCGCTGGAAGCTTATCAACAATTA
CGCTGGACACGGAATTCGCAGGGTGTACAACTCACTAACCCAACGCCTTACTACATTAACCTGATTCAGGTGAGCGTAAA
TGGTAAAGCGTTAAGTAATGCGGGAGTTGTGCCACCTAAAAGCCAGCGTCAGACAAGCTGGTGTCAGGCTATAACACCGT
GTCATGTCTCCTGGCGGACGATAAATGATTACGGCGGGTTGAGTGCAAAGAAGGAGCAAAATCTGCCCTGA

Upstream 100 bases:

>100_bases
AGCGGTAAGGTGCCATTAACTTTTTACGCGCAATTGCGACCTGTCAATAGCGATGTGCAGTCGGGTAAAGTGAATGCCAG
CGCGACATTTGTACTTCATT

Downstream 100 bases:

>100_bases
AACAGGCTCGGAAAACGTTTGCGCTTTTTTTGCCACAGGTCAATTCCCTTTTGGTCCGAACTCGCACATAATACGCCCCC
GGTTTGCACACCGGGAATCC

Product: putativi pili assembly chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTNTWNRLALLIFAVLSLLVASELQAGVVVGGTRFIFPADRDSISISLTNTSQESWLINSKINRPTRWAGGEASTVPAPL
LAAPPLILLKPGTTGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPGDPLEAYQQL
RWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQAITPCHVSWRTINDYGGLSAKKEQNLP

Sequences:

>Translated_236_residues
MTNTWNRLALLIFAVLSLLVASELQAGVVVGGTRFIFPADRDSISISLTNTSQESWLINSKINRPTRWAGGEASTVPAPL
LAAPPLILLKPGTTGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPGDPLEAYQQL
RWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQAITPCHVSWRTINDYGGLSAKKEQNLP
>Mature_235_residues
TNTWNRLALLIFAVLSLLVASELQAGVVVGGTRFIFPADRDSISISLTNTSQESWLINSKINRPTRWAGGEASTVPAPLL
AAPPLILLKPGTTGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPGDPLEAYQQLR
WTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQAITPCHVSWRTINDYGGLSAKKEQNLP

Specific function: Could be required for the biogenesis of a putative fimbria [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI87081806, Length=236, Percent_Identity=97.0338983050847, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1790771, Length=223, Percent_Identity=32.2869955156951, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787171, Length=205, Percent_Identity=34.6341463414634, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1786743, Length=236, Percent_Identity=28.3898305084746, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1786333, Length=239, Percent_Identity=30.5439330543933, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789532, Length=228, Percent_Identity=27.6315789473684, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI87082203, Length=239, Percent_Identity=26.3598326359833, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1786936, Length=195, Percent_Identity=30.7692307692308, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788428, Length=237, Percent_Identity=22.7848101265823, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788677, Length=176, Percent_Identity=27.8409090909091, Blast_Score=71, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 25891; Mature: 25760

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTWNRLALLIFAVLSLLVASELQAGVVVGGTRFIFPADRDSISISLTNTSQESWLINS
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEECCCCEEEEEEECCCCCCEEEEC
KINRPTRWAGGEASTVPAPLLAAPPLILLKPGTTGTLRLLRTESDILPVDRETLFELSIA
CCCCCCCCCCCCCCCCCCCHHHCCCEEEECCCCCCEEEEEECCCCCCCCCHHHHHEEEEC
SVPSGKVENQSVKVAMRSVFKLFWRPEGLPGDPLEAYQQLRWTRNSQGVQLTNPTPYYIN
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCEEEE
LIQVSVNGKALSNAGVVPPKSQRQTSWCQAITPCHVSWRTINDYGGLSAKKEQNLP
EEEEEECCCEECCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
TNTWNRLALLIFAVLSLLVASELQAGVVVGGTRFIFPADRDSISISLTNTSQESWLINS
CCCHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEECCCCEEEEEEECCCCCCEEEEC
KINRPTRWAGGEASTVPAPLLAAPPLILLKPGTTGTLRLLRTESDILPVDRETLFELSIA
CCCCCCCCCCCCCCCCCCCHHHCCCEEEECCCCCCEEEEEECCCCCCCCCHHHHHEEEEC
SVPSGKVENQSVKVAMRSVFKLFWRPEGLPGDPLEAYQQLRWTRNSQGVQLTNPTPYYIN
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCEEEE
LIQVSVNGKALSNAGVVPPKSQRQTSWCQAITPCHVSWRTINDYGGLSAKKEQNLP
EEEEEECCCEECCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 2992959; 7984428 [H]