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The map label for this gene is helD

Identifier: 218700518

GI number: 218700518

Start: 2227983

End: 2230037

Strand: Reverse

Name: helD

Synonym: ECIAI39_2184

Alternate gene names: 218700518

Gene position: 2230037-2227983 (Counterclockwise)

Preceding gene: 218700521

Following gene: 218700514

Centisome position: 43.45

GC content: 52.9

Gene sequence:

>2055_bases
ATGGAACTGAAAGCGACAACGCTTGGAAAACGTCTGGCACAGCACCCTTACGATCGGGCGGTGATCCTCAATGCCGGGAT
TAAAGTCTCCGGCGATCGCCACGAATACCTTATTCCTTTCAATCAATTACTGGCGATTCACTGTAAGCGCGGTCTGGTAT
GGGGCGAGCTGGAATTTGTACTGCCGGGCGAAAAAGTGGTGCGTCTGCACGGCACCGAATGGGGCGAGACGCAGCGTTTT
TACCATCATCTTGATACTCACTGGCGGCGGTGGAGTGGGGAGATGAGCGAAATTGCGTCTGGCGTTTTACGTCAGCAACT
GGATTTGATTGCCACGCGCACCGGAGAAAATAAATGGCTGACGCGTGAGCAAACCTCTGGTGTGCAGCAACAAATCCGCC
AGGCTTTGTCGGCGTTGCCGTTGCCGGTTAACCGACTGGAAGAATTCGATAACTGCCGTGAGGCGTGGCGTAAATGTCAG
GCCTGGTTGAAGGACATTGAAGGCGCTCGGTTGCAGCATAATCAGGCGTATACCGAAGCCATGCTTACCGAGTATGCGGA
TTTTTTCCGCCAGGTCGAATCTTCACCGCTGAATCCGGCGCAGGCCCGGGCAGTCGTTAATGGCGAGCATTCTTTGTTAG
TGCTGGCTGGTGCAGGAAGCGGAAAAACGTCGGTGCTGGTGGCCCGTGCAGGCTGGTTACTGGCGCGTGGTGAAGCGTCC
CCTGAGCAAATTTTATTGCTGGCGTTTGGTCGCAAAGCCGCTGAAGAGATGGACGAGCGTATTCGCGAACGGCTACATAC
CGAAGACATTACCGCACGCACGTTTCATGCGCTGGCGCTGCATATTATTCAGCAGGGCAGCAAAAAAGTTCCGATAGTCA
GCAAACTGGAAAATGATACCGCTGCCCGTCATGAACTCTTTATTGCTGAGTGGCGCAAGCAATGCAGCGAAAAGAAAGCG
CAGGCGAAGGGCTGGCGGCAATGGCTGACGGAAGAAATGCAGTGGTCAGTGCCAGCAGGTAACTTCTGGGATGATGAAAA
ATTACAGCGTCGTCTTGCCTCACGCCTCGATCGTTGGGTAAGTCTGATGCGGATGCACGGTGGTGCACAGGCAGAAATGA
TTGCCAGTGCACCCGAAGAGATTCGCGATCTGTTCAGTAAACGTATCAAGTTGATGGCTCCGTTATTAAAAGCCTGGAAA
GGTGCGCTGAAGGCAGAAAACGCTGTCGATTTTTCGGGCCTTATTCACCAGGCGATTGTGATTCTGGAGAAAGGTCGCTT
TATCAGCCCGTGGAAGCATATTCTGGTTGATGAATTTCAGGATATCTCGCCGCAGCGCGCAGCGTTGTTAGCGGCATTAC
GCAAGCAAAACAGTCAGACGACGTTGTTCGCCGTTGGTGATGACTGGCAGGCGATTTATCGCTTCAGCGGTGCGCAAATG
TCGCTCACCACCGCTTTCCATGAAAACTTTGGTGAAGGTGATCGCTGCGATTTAGACACGACTTACCGTTTTAACAGTCG
TATCGGTGAGGTGGCAAACAGGTTTATTCAGCAGAACCCAGGCCAGCTGAAAAAGCCGCTAAACAGCTTAACCAATGGAG
ACAAAAAAGCCGTCACGTTATTGGATGAGAGCCATCTTGACGCTTTGCTGGATAAGCTCTCTGGTTATGCCAAACCGGAA
GAGCGCATTCTGATCCTGGCGCGTTACCATCACATGAGGCCTGCCAGCCTGGAAAAAGCGGCAACACGCTGGCCGAAGTT
GCAAATCGACTTTATGACCATTCATGCCAGCAAAGGGCAACAGGCGGATTACGTCATCATCGTTGGCTTGCAGGAGGGGA
GTGATGGTTTTCCGGCTGCGGCGCGGGAGTCGATTATGGAAGAGGCGCTACTGCCACCGGTTGAGGATTTCCCGGACGCT
GAAGAACGGCGGTTAATGTACGTGGCGCTGACCCGGGCACGCCATCGGGTATGGGCACTGTTTAACAAAGAGAATCCCTC
TCCATTTGTCGAAATACTGAAAAATCTGGATGTGCCGGTGGCGAGAAAACCGTAA

Upstream 100 bases:

>100_bases
TTCAGTAATGGGCGGCAATTGTAACGTTTTTTTGGGCTGGAGCACGTTTTCTCTGACGGTTACACTGATAAGAAATCATT
TCGTGTGGATCTACAGAGTC

Downstream 100 bases:

>100_bases
GAAACAGGTGGCGTTTGCCACCTGTGCAATATTACTTCAGACGGTCCGCGAGATAACGCTGATAATCGGGGATCAGAATA
TCGACCGCATCGTTGAAATG

Product: DNA helicase IV

Products: NA

Alternate protein names: 75 kDa helicase [H]

Number of amino acids: Translated: 684; Mature: 684

Protein sequence:

>684_residues
MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLLAIHCKRGLVWGELEFVLPGEKVVRLHGTEWGETQRF
YHHLDTHWRRWSGEMSEIASGVLRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGAGSGKTSVLVARAGWLLARGEAS
PEQILLLAFGRKAAEEMDERIRERLHTEDITARTFHALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKA
QAKGWRQWLTEEMQWSVPAGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLMAPLLKAWK
GALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALLAALRKQNSQTTLFAVGDDWQAIYRFSGAQM
SLTTAFHENFGEGDRCDLDTTYRFNSRIGEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPE
ERILILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARESIMEEALLPPVEDFPDA
EERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVARKP

Sequences:

>Translated_684_residues
MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLLAIHCKRGLVWGELEFVLPGEKVVRLHGTEWGETQRF
YHHLDTHWRRWSGEMSEIASGVLRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGAGSGKTSVLVARAGWLLARGEAS
PEQILLLAFGRKAAEEMDERIRERLHTEDITARTFHALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKA
QAKGWRQWLTEEMQWSVPAGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLMAPLLKAWK
GALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALLAALRKQNSQTTLFAVGDDWQAIYRFSGAQM
SLTTAFHENFGEGDRCDLDTTYRFNSRIGEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPE
ERILILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARESIMEEALLPPVEDFPDA
EERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVARKP
>Mature_684_residues
MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLLAIHCKRGLVWGELEFVLPGEKVVRLHGTEWGETQRF
YHHLDTHWRRWSGEMSEIASGVLRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGAGSGKTSVLVARAGWLLARGEAS
PEQILLLAFGRKAAEEMDERIRERLHTEDITARTFHALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKA
QAKGWRQWLTEEMQWSVPAGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLMAPLLKAWK
GALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALLAALRKQNSQTTLFAVGDDWQAIYRFSGAQM
SLTTAFHENFGEGDRCDLDTTYRFNSRIGEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESHLDALLDKLSGYAKPE
ERILILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARESIMEEALLPPVEDFPDA
EERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVARKP

Specific function: Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787196, Length=684, Percent_Identity=99.1228070175439, Blast_Score=1394, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=340, Percent_Identity=28.2352941176471, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI48994965, Length=341, Percent_Identity=28.1524926686217, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6322369, Length=352, Percent_Identity=28.125, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000212
- InterPro:   IPR014016
- InterPro:   IPR022161
- InterPro:   IPR003661 [H]

Pfam domain/function: PF12462 Nucleolin_N; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77856; Mature: 77856

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLLAIHCKRGLVWGELEFV
CCCCHHHHHHHHHHCCCCCEEEEECCEEECCCCCEEECCHHHHHHHHHHCCCEEEEEEEE
LPGEKVVRLHGTEWGETQRFYHHLDTHWRRWSGEMSEIASGVLRQQLDLIATRTGENKWL
ECCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
TREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQAWLKDIEGARLQHNQAYTEA
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
MLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGAGSGKTSVLVARAGWLLARGEAS
HHHHHHHHHHHHHCCCCCHHHHHEEECCCCEEEEEEECCCCCCEEEEEECCEEEECCCCC
PEQILLLAFGRKAAEEMDERIRERLHTEDITARTFHALALHIIQQGSKKVPIVSKLENDT
HHHEEEEHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCH
AARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQWSVPAGNFWDDEKLQRRLASRLDRWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
SLMRMHGGAQAEMIASAPEEIRDLFSKRIKLMAPLLKAWKGALKAENAVDFSGLIHQAIV
HHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ILEKGRFISPWKHILVDEFQDISPQRAALLAALRKQNSQTTLFAVGDDWQAIYRFSGAQM
HHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCE
SLTTAFHENFGEGDRCDLDTTYRFNSRIGEVANRFIQQNPGQLKKPLNSLTNGDKKAVTL
EEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEE
LDESHLDALLDKLSGYAKPEERILILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQ
ECHHHHHHHHHHHCCCCCCHHHEEEEEHHCCCCCCHHHHHHHCCCCEEEEEEEEECCCCC
QADYVIIVGLQEGSDGFPAAARESIMEEALLPPVEDFPDAEERRLMYVALTRARHRVWAL
CCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHEEEHHHHHHHHHHEEEE
FNKENPSPFVEILKNLDVPVARKP
ECCCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLLAIHCKRGLVWGELEFV
CCCCHHHHHHHHHHCCCCCEEEEECCEEECCCCCEEECCHHHHHHHHHHCCCEEEEEEEE
LPGEKVVRLHGTEWGETQRFYHHLDTHWRRWSGEMSEIASGVLRQQLDLIATRTGENKWL
ECCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
TREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQAWLKDIEGARLQHNQAYTEA
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
MLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGAGSGKTSVLVARAGWLLARGEAS
HHHHHHHHHHHHHCCCCCHHHHHEEECCCCEEEEEEECCCCCCEEEEEECCEEEECCCCC
PEQILLLAFGRKAAEEMDERIRERLHTEDITARTFHALALHIIQQGSKKVPIVSKLENDT
HHHEEEEHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCCCH
AARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQWSVPAGNFWDDEKLQRRLASRLDRWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
SLMRMHGGAQAEMIASAPEEIRDLFSKRIKLMAPLLKAWKGALKAENAVDFSGLIHQAIV
HHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
ILEKGRFISPWKHILVDEFQDISPQRAALLAALRKQNSQTTLFAVGDDWQAIYRFSGAQM
HHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCE
SLTTAFHENFGEGDRCDLDTTYRFNSRIGEVANRFIQQNPGQLKKPLNSLTNGDKKAVTL
EEHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEE
LDESHLDALLDKLSGYAKPEERILILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQ
ECHHHHHHHHHHHCCCCCCHHHEEEEEHHCCCCCCHHHHHHHCCCCEEEEEEEEECCCCC
QADYVIIVGLQEGSDGFPAAARESIMEEALLPPVEDFPDAEERRLMYVALTRARHRVWAL
CCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHEEEHHHHHHHHHHEEEE
FNKENPSPFVEILKNLDVPVARKP
ECCCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2542273; 8905232; 9278503 [H]