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The map label for this gene is hyaD

Identifier: 218700505

GI number: 218700505

Start: 2218150

End: 2218737

Strand: Reverse

Name: hyaD

Synonym: ECIAI39_2171

Alternate gene names: 218700505

Gene position: 2218737-2218150 (Counterclockwise)

Preceding gene: 218700506

Following gene: 218700504

Centisome position: 43.23

GC content: 59.35

Gene sequence:

>588_bases
ATGAGCGAGCAACGCGTGGTGGTAATGGGGCTGGGTAACCTGCTGTGGGCCGATGAAGGCTTCGGCGTGCGGGTGGCGGA
ACGGCTGTATGCCCATTACCACTGGCCCGATGAAGTCGAGATTGTTGATGGCGGCACTCAGGGACTGAACTTGCTGGGGT
ATGTCGAAAGCGCCAGCCACTTGCTGATTCTCGACGCCATTGATTACGGGCTGGAACCCGGAACGCTGCGAACCTATGCC
GGAGAACGCATTCCGGCCTATCTCAGCGCGAAGAAAATGAGCCTGCACCAGAACAGTTTCTCCGAAGTGCTGGCGCTGGC
AGATATCCGCGGACATCTGCCAGCACATATTGCCCTCGTCGGTCTGCAACCCGCGATGCTCGACGACTACGGCGGCAGCC
TGAGCGAACTGGCGCGGGAGCAACTGCCCGCCGCGGAACAGGCGGCGCTGGTACAGCTGGCGGCCTGGGGAATTGTACCG
CAACCGACTGAGGAGTTGCGCTGTCTCAATTATGACTGTCTGTCGATGGAAAATTACGAAGGCGTTCGCTTGCGTCAGTA
CAAGATGAGGCTGGAGGAACAGGGATGA

Upstream 100 bases:

>100_bases
CGCTGCGTGAAGACATCATGTCCGACGACACGGTGATCTCCACTATGGTCAACGGCTACCGTAGCCACAAATTTGGCAAA
ATAAGTAACAAGGAGCGTTC

Downstream 100 bases:

>100_bases
GCAACGACACACCATTTGATGCGTTGTGGCAACGAATGCTGGCGCGCGGCTGGACGCCGATCAGTGAATCCCGTCTTGAC
GACTGGCTTACGCAAGCGCC

Product: hydrogenase 1 maturation protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDEVEIVDGGTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYA
GERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALVQLAAWGIVP
QPTEELRCLNYDCLSMENYEGVRLRQYKMRLEEQG

Sequences:

>Translated_195_residues
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDEVEIVDGGTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYA
GERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALVQLAAWGIVP
QPTEELRCLNYDCLSMENYEGVRLRQYKMRLEEQG
>Mature_194_residues
SEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDEVEIVDGGTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAG
ERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALVQLAAWGIVPQ
PTEELRCLNYDCLSMENYEGVRLRQYKMRLEEQG

Specific function: Protease involved in the C-terminal processing of hyaB, the large subunit of hydrogenase 1 (Potential) [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1787209, Length=195, Percent_Identity=95.3846153846154, Blast_Score=355, Evalue=1e-99,
Organism=Escherichia coli, GI1789367, Length=163, Percent_Identity=38.0368098159509, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004419
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 21610; Mature: 21479

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDEVEIVDGGTQGLNLLGYVESASH
CCCCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEECCCCE
LLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALV
EEEEEEHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
GLQPAMLDDYGGSLSELAREQLPAAEQAALVQLAAWGIVPQPTEELRCLNYDCLSMENYE
ECCCHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCEECCCCCC
GVRLRQYKMRLEEQG
CCHHHHHHHHHHCCC
>Mature Secondary Structure 
SEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPDEVEIVDGGTQGLNLLGYVESASH
CCCEEEEEECCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCEEEEEECCCCE
LLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSEVLALADIRGHLPAHIALV
EEEEEEHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
GLQPAMLDDYGGSLSELAREQLPAAEQAALVQLAAWGIVPQPTEELRCLNYDCLSMENYE
ECCCHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCEECCCCCC
GVRLRQYKMRLEEQG
CCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ni [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180913; 8905232; 9278503 [H]