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The map label for this gene is chbA [H]

Identifier: 218700182

GI number: 218700182

Start: 1913563

End: 1913880

Strand: Reverse

Name: chbA [H]

Synonym: ECIAI39_1831

Alternate gene names: 218700182

Gene position: 1913880-1913563 (Counterclockwise)

Preceding gene: 218700183

Following gene: 218700181

Centisome position: 37.29

GC content: 44.65

Gene sequence:

>318_bases
ATGTTGATGTTTGCAGATGAAGAACTTGTTATGGAGTTACTGGTCAACGCAGGACAGGCCCGTTCTGATGCGATGGAAGC
CATACGTTGTGCCGGGCAAAAAGACTGGCAGGGCGCAACTCAGCTAATGGCCAGTTCTGAATCCGCCTGTCTTCAGGCGC
ATAAGATTCAGACCGCGTTAATTAGTCAGGATGAAGGCTGTGGCAAAATAGAAGTTAATTTAATTCTGGTTCATGCACAG
GATCATTTGATGAATGCAATATTATGCCAGGATTTAGCCAGGGAAATTATTTCATTAAGAAAAGAGCTCCATGCCTGA

Upstream 100 bases:

>100_bases
TTTAGGTGTGGCGACAATGATCTATTTACCTTTTGTTATTGCCGCCAACAAGGTCAATAACTTAACAACTAACGGATAAT
GCCATTTACACAGGAAGGAG

Downstream 100 bases:

>100_bases
TTTTTTCCTCCAGTGCGATAGCTGGAGGAAATTATTACACTCTTAAGGATAAGTCATGAATATAAAGACGCTTAACGTCA
GCCTTTTGTCTTTTTCTATT

Product: putative phosphotransferase system PTS, lactose/cellobiose-specific IIA subunit

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIA-Chb; EIII-Chb; PTS system N,N'-diacetylchitobiose-specific EIIA component [H]

Number of amino acids: Translated: 105; Mature: 105

Protein sequence:

>105_residues
MLMFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASSESACLQAHKIQTALISQDEGCGKIEVNLILVHAQ
DHLMNAILCQDLAREIISLRKELHA

Sequences:

>Translated_105_residues
MLMFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASSESACLQAHKIQTALISQDEGCGKIEVNLILVHAQ
DHLMNAILCQDLAREIISLRKELHA
>Mature_105_residues
MLMFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASSESACLQAHKIQTALISQDEGCGKIEVNLILVHAQ
DHLMNAILCQDLAREIISLRKELHA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG1447

COG function: function code G; Phosphotransferase system cellobiose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788031, Length=99, Percent_Identity=48.4848484848485, Blast_Score=91, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003188 [H]

Pfam domain/function: PF02255 PTS_IIA [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 11548; Mature: 11548

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS51095 PTS_EIIA_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
9.5 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
9.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASSESACLQAHKIQTAL
CCEECCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHH
ISQDEGCGKIEVNLILVHAQDHLMNAILCQDLAREIISLRKELHA
HCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLMFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASSESACLQAHKIQTAL
CCEECCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHH
ISQDEGCGKIEVNLILVHAQDHLMNAILCQDLAREIISLRKELHA
HCCCCCCCEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]