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The map label for this gene is ydeO

Identifier: 218700128

GI number: 218700128

Start: 1860082

End: 1860843

Strand: Reverse

Name: ydeO

Synonym: ECIAI39_1764

Alternate gene names: 218700128

Gene position: 1860843-1860082 (Counterclockwise)

Preceding gene: 218700129

Following gene: 218700127

Centisome position: 36.26

GC content: 39.76

Gene sequence:

>762_bases
ATGTCGCTCGTTTGTTCTGTTATATTTATTCATCATGCCTTCAACGCTAACATTTTAGATAAAGATTACGCCTTCTCTGA
CGGCGAGATCCTGATGGTAGATAACGCTGTTCGTACGCATTTTGAACCTTATGAGCGGCATTTCAAAGAAATCGGATTTA
ATGAAAATACCATTAAAAAATATCTACAATGCACTAACATTCAGACAGTGACGGTGCCTGTTCCTGCGAAGTTTTTACGT
GCTTCAAATGTACCGACTGGATTGCTTAATGAAATGATTGCTTATCTCAACTCGGAAGAACGCAATCATCATAATTTTTC
AGAACTTTTGCTTTTTTCTTGCCTGTCTATTTTTGCCGCATGCAAAGGTTTCATTACACTATTAACTAACGGTGTGCTAT
CCGTTTCCGGGAAAGTGAGAAATATTGTCAACATGAAGCTGGCGCACCCATGGAAGCTGAAAGATATTTGTGACTGCCTG
TACATCAGTGAAAGCCTGTTGAAGAAAAAACTTAAGCAAGAGCAAACGACATTCTCACAGATTCTTTTAGATGCCAGAAT
GCAGCACGCAAAAAATTTGATACGCGTAGAAGGTTCAGTCAATAAAATTGCCGAACAATGTGGTTATGCCAGTACATCTT
ATTTTATTTATGCGTTCCGCAAACATTTCGGCAACAGCCCGAAGAGAGTTTCTAAGGAGTACCGTTGTCAAAGGCACACC
GGTATGAACACGGACAACACGATGAGTGCTTTAGCTATTTGA

Upstream 100 bases:

>100_bases
TCATTATCAATGGTTTGCAAGACTGAAAATAAACAGCAAATTATCAATATGAAATGTTAAAAAAGTATCGATAAAAACTT
TATTGTTTTAAGGAGATAAA

Downstream 100 bases:

>100_bases
TTATTTGCTAACGAGTAGTCAACCACACACGCTACGTAGAAATTAAAATGGGGCAGCCATTCCCTGCCCCGCGTTGTTTT
TAGGCGATATATTTATTGAA

Product: transcriptional regulator YdeO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLR
ASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHT
GMNTDNTMSALAI

Sequences:

>Translated_253_residues
MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLR
ASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHT
GMNTDNTMSALAI
>Mature_252_residues
SLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRA
SNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLY
ISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHTG
MNTDNTMSALAI

Specific function: Induces the expression of gadE and mdtEF. Could also regulate the expression of other genes involved in acid resistance [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787776, Length=253, Percent_Identity=98.0237154150198, Blast_Score=517, Evalue=1e-148,
Organism=Escherichia coli, GI1789932, Length=237, Percent_Identity=34.1772151898734, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786776, Length=235, Percent_Identity=28.936170212766, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789933, Length=146, Percent_Identity=42.4657534246575, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790557, Length=144, Percent_Identity=34.7222222222222, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1786778, Length=124, Percent_Identity=38.7096774193548, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 28855; Mature: 28724

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKK
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHCCCHHHHHHHHCCCCHHHHHH
YLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAA
HHHHCCCEEEEECCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
CKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQ
HHHHHHHHHCCHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHT
HHHHHHHHHHHHHEEECCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GMNTDNTMSALAI
CCCCCCCHHHHCC
>Mature Secondary Structure 
SLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKK
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHCCCHHHHHHHHCCCCHHHHHH
YLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAA
HHHHCCCEEEEECCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
CKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFSQ
HHHHHHHHHCCHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQRHT
HHHHHHHHHHHHHEEECCHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GMNTDNTMSALAI
CCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]