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The map label for this gene is sfcA

Identifier: 218700109

GI number: 218700109

Start: 1831114

End: 1832811

Strand: Reverse

Name: sfcA

Synonym: ECIAI39_1743

Alternate gene names: 218700109

Gene position: 1832811-1831114 (Counterclockwise)

Preceding gene: 218700111

Following gene: 218700108

Centisome position: 35.71

GC content: 52.12

Gene sequence:

>1698_bases
ATGGAACCAAAAACAAAAAAACAGCGTTCGCTTTATATCCCTTACGCTGGCCCTGTACTGCTGGAATTTCCGTTGTTGAA
TAAAGGCAGTGCCTTCAGCATGGAAGAACGCCGTAACTTCAACCTGCTGGGGTTACTGCCGGAAGTGGTCGAAACCATCG
AAGAACAAGCGGAACGAGCATGGATCCAGTATCAGGGATTCAAAACCGAAATCGACAAACACATCTACCTGCGTAACATC
CAGGACACTAACGAAACCCTCTTCTACCGTCTGGTAAACAATCATCTTGATGAGATGATGCCTGTTATTTATACCCCAAC
CGTCGGCGCAGCCTGTGAGCGTTTTTCTGAGATCTACCGCCGTTCACGCGGCGTATTTATCTCTTACCAGAACCGCCACA
ATATGGACGATATTCTGCAAAACGTACCAAACCATAATATTAAAGTGATTGTGGTGACTGACGGCGAACGTATTCTGGGG
CTTGGTGACCAGGGCATCGGCGGGATGGGCATTCCGATCGGTAAACTGTCACTCTATACCGCATGTGGCGGCATCAGCCC
GGCGTATACCCTTCCGGTGGTGCTGGATGTCGGGACGAACAACCAACAGCTGCTTAACGATCCGCTGTATATGGGCTGGC
GTAATCCGCGTATCACTGACGACGAATACTATGAATTCGTTGATGAATTTATCCAGGCTGTGAAACAACGCTGGCCGGAC
GTACTGTTGCAGTTTGAAGACTTCGCACAAAAAAATGCGATGCCGTTACTTAACCGCTATCGCAATGAAATTTGTTCTTT
TAACGATGACATTCAGGGCACCGCGGCGGTAACGGTCGGCACACTGATCGCAGCCAGCCGCGCGGCGGGTGGTCAACTGA
GCGAGAAAAAGATTGTCTTCCTCGGCGCAGGCTCTGCGGGGTGCGGGATTGCCGAAATGATCATCGCCCAGACCCAGCGC
GAAGGATTAAGCGAGGAAGCGGCGCGGCAGAAAGTCTTTATGGTCGATCGCTTTGGCCTGCTGACCGACAAGATGCCGAA
CCTGCTGCCTTTCCAGACCAAACTGGTGCAGAAACGCGAAAACCTCAGCGACTGGGATACCGACAGCGATGTGCTGTCAC
TGCTGGATGTGGTGCGCAATGTAAAACCAGATATTCTGATTGGCGTCTCAGGACAGACCGGGCTGTTTACGGAAGAGATC
ATCCGTGAGATGCACAAACACTGTCCGCGTCCGATCGTGATGCCGCTGTCTAACCCGACTTCACGCGTCGAAGCAACACC
GCAGGACATTATCGCCTGGACTGAAGGTAACGCGCTGGTCGCCACTGGCAGTCCATTTAATCCAGTGGTATGGAAAGATA
AAATCTACCCTATCGCCCAGTGTAATAACGCCTTTATTTTCCCGGGCATCGGGCTGGGTGTTATTGCTTCCGGCGCGTCA
CGTATCACCGATGAAATGCTGATGTCGGCAAGTGAAACGCTTGCTCAGTATTCGCCGCTGGTCCTGAACGGCGAAGGTCT
GGTACTACCGGAACTGAAAGATATACAGAAAGTCTCCCGCGCAATTGCGTTTGCGGTTGGCAAAATGGCGCAGCAGCAAG
GCGTGGCGGTGAAAACCTCCGCCGAAGCTCTGCAACAGGCCATTGATGATAATTTCTGGCACGCCGAATACCGCGACTAC
CGCCGTACCTCCATCTAA

Upstream 100 bases:

>100_bases
TTTGTTTTTATCTGCTTTATACTTGGGGCCGACGCCCTGGCGGTAAAGCAAAGACGATAAAAGCGTGCCAGGGATGGATA
TTCAAAAAAGAGTGAGTGAC

Downstream 100 bases:

>100_bases
GCCTGCGCCCGGTAGTGAAGGCTACCGGGCAATTTCCCTCTCCCTTTTTCAGATCTCATCCATACTGGGTAGTGGCGAAT
AAATCTCATTTGCCTCACCT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNI
QDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD
VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQR
EGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEI
IREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGAS
RITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDY
RRTSI

Sequences:

>Translated_565_residues
MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNI
QDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD
VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQR
EGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEI
IREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGAS
RITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDY
RRTSI
>Mature_565_residues
MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNI
QDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD
VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQR
EGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEI
IREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGAS
RITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDY
RRTSI

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=540, Percent_Identity=42.7777777777778, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI62420882, Length=515, Percent_Identity=44.0776699029126, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI62420880, Length=515, Percent_Identity=44.0776699029126, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI239049447, Length=515, Percent_Identity=44.0776699029126, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI4505145, Length=552, Percent_Identity=40.9420289855072, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI270265879, Length=463, Percent_Identity=44.060475161987, Blast_Score=391, Evalue=1e-108,
Organism=Escherichia coli, GI87081919, Length=565, Percent_Identity=99.4690265486726, Blast_Score=1166, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17537199, Length=567, Percent_Identity=43.9153439153439, Blast_Score=433, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322823, Length=521, Percent_Identity=45.6813819577735, Blast_Score=437, Evalue=1e-123,
Organism=Drosophila melanogaster, GI281362672, Length=516, Percent_Identity=44.7674418604651, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI21356279, Length=516, Percent_Identity=44.7674418604651, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI281362674, Length=516, Percent_Identity=44.7674418604651, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24646388, Length=512, Percent_Identity=42.1875, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI24646386, Length=512, Percent_Identity=42.1875, Blast_Score=430, Evalue=1e-120,
Organism=Drosophila melanogaster, GI78707242, Length=537, Percent_Identity=38.9199255121043, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI78707238, Length=537, Percent_Identity=38.9199255121043, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI78707232, Length=507, Percent_Identity=38.2642998027613, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI281363505, Length=507, Percent_Identity=38.2642998027613, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI78707236, Length=507, Percent_Identity=38.2642998027613, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI78707240, Length=513, Percent_Identity=39.766081871345, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI281363503, Length=505, Percent_Identity=38.019801980198, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI19922384, Length=536, Percent_Identity=31.3432835820896, Blast_Score=275, Evalue=4e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 63173; Mature: 63173

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERA
CCCCCCCCCEEECCCCCCEEEEECCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHH
WIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYR
HHHCCCCHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHH
RSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYT
HCCCEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHH
ACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD
HHCCCCCCEEEEEEEEECCCCHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCHH
VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVF
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEE
LGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKLVQKRE
EECCCCCCHHHHHHHHHHHHCCCHHHHHHHHEEEHHHHHHHHHCCCCCCCHHHHHHHHHC
NLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIVMPLSNPT
CCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCH
SRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGAS
HHCCCCHHHEEEEECCCEEEECCCCCCCCEECCCCCCCEECCCEEEECCCCHHHHHCCHH
RITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAVKTS
HHHHHHHHHHHHHHHHCCCEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHH
AEALQQAIDDNFWHAEYRDYRRTSI
HHHHHHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIEEQAERA
CCCCCCCCCEEECCCCCCEEEEECCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHH
WIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAACERFSEIYR
HHHCCCCHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHH
RSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYT
HCCCEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHHHHH
ACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVDEFIQAVKQRWPD
HHCCCCCCEEEEEEEEECCCCHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCHH
VLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAASRAAGGQLSEKKIVF
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEE
LGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFMVDRFGLLTDKMPNLLPFQTKLVQKRE
EECCCCCCHHHHHHHHHHHHCCCHHHHHHHHEEEHHHHHHHHHCCCCCCCHHHHHHHHHC
NLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIREMHKHCPRPIVMPLSNPT
CCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCH
SRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQCNNAFIFPGIGLGVIASGAS
HHCCCCHHHEEEEECCCEEEECCCCCCCCEECCCCCCCEECCCEEEECCCCHHHHHCCHH
RITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKVSRAIAFAVGKMAQQQGVAVKTS
HHHHHHHHHHHHHHHHCCCEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHH
AEALQQAIDDNFWHAEYRDYRRTSI
HHHHHHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA