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The map label for this gene is ldhA

Identifier: 218700074

GI number: 218700074

Start: 1780259

End: 1781248

Strand: Reverse

Name: ldhA

Synonym: ECIAI39_1705

Alternate gene names: 218700074

Gene position: 1781248-1780259 (Counterclockwise)

Preceding gene: 218700085

Following gene: 218700069

Centisome position: 34.71

GC content: 49.7

Gene sequence:

>990_bases
ATGAAACTCGCCGTTTATAGCACAAAACAGTACGACAAGAAGTACCTGCAACAGGTGAACGAGTCCTTTGGCTTTGAGCT
GGAATTTTTTGACTTTCTGCTGACGGAAAAAACCGCTAAAACTGCCAATGGCTGCGAAGCGGTATGTATTTTCGTAAACG
ATGACGGCAGCCGCCCGGTGCTGGAAGAGCTGAAAAAGCACGGCGTTAAATATATCGCCCTGCGCTGTGCCGGTTTCAAT
AACGTCGACCTTGACGCGGCAAAAGAACTGGGGCTGAAAGTAGTCCGTGTTCCAGCCTATGATCCAGAAGCTGTTGCTGA
ACACGCCATCGGTATGATGATGACGCTGAACCGCCGTATCCATCGCGCATATCAGCGTACGCGTGACGCCAACTTCTCTC
TGGAAGGTCTGACCGGCTTTACCATGTATGGCAAAACGGCAGGCGTTATCGGTACTGGTAAAATCGGTGTGGCGATGCTG
CGCATTCTCAAAGGTTTTGGTATGCGTCTGCTGGCGTTCGATCCGTATCCAAGTGCGGCGGCACTGGAACTCGGTGTGGA
GTATGTCGATCTGCCAACCCTGTTCTCTGAATCAGACGTTATCTCTCTGCACTGCCCGCTGACACCGGAAAACTATCATC
TGTTGAACGAAGCTGCCTTCGAACAGATGAAAAATGGCGTGATGATCGTCAATACCAGTCGCGGTGCATTGATTGATTCT
CAGGCAGCAATTGAAGCGCTGAAAAATCAGAAAATTGGTTCGTTGGGTATGGACGTGTATGAGAACGAACGCGATCTGTT
CTTTGAAGATAAATCCAACGACGTAATTCAGGATGACGTATTCCGTCGCCTGTCTGCCTGCCACAACGTGCTGTTTACCG
GGCATCAGGCATTCCTGACAGCAGAAGCTCTGACCAGTATTTCTCAGACTACGCTGCAAAACTTAAGCAATCTGGAAAAA
GGCGAAACCTGCCCGAACGAACTGGTTTAA

Upstream 100 bases:

>100_bases
ATGAATTTTTCAATATCGCCATAGCTTTCAATTATATTTGAAATTTTGTAAAATATTTTTAGTAGCTTAAATGTGATTCA
ACATCACTGGAGAAAGTCTT

Downstream 100 bases:

>100_bases
TCTTGCCGCTCCCCTGCATTCCAGGGGAGCTTATTCAGATAATCCCCAATGACCTTTCATCCTCTATTCTTAAAATAGTC
CTGAGTCAGAAACTGTAATT

Product: fermentative D-lactate dehydrogenase, NAD-dependent

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFN
NVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAFEQMKNGVMIVNTSRGALIDS
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEK
GETCPNELV

Sequences:

>Translated_329_residues
MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFN
NVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAFEQMKNGVMIVNTSRGALIDS
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEK
GETCPNELV
>Mature_329_residues
MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFN
NVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAFEQMKNGVMIVNTSRGALIDS
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEK
GETCPNELV

Specific function: Fermentative lactate dehydrogenase

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Homologues:

Organism=Homo sapiens, GI23308577, Length=200, Percent_Identity=34, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI61743967, Length=252, Percent_Identity=32.5396825396825, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI4557497, Length=252, Percent_Identity=32.5396825396825, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI145580578, Length=252, Percent_Identity=32.5396825396825, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI4557499, Length=252, Percent_Identity=32.5396825396825, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI145580575, Length=248, Percent_Identity=33.0645161290323, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI6912396, Length=201, Percent_Identity=31.8407960199005, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1787645, Length=329, Percent_Identity=100, Blast_Score=682, Evalue=0.0,
Organism=Escherichia coli, GI87082289, Length=269, Percent_Identity=27.5092936802974, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789279, Length=232, Percent_Identity=29.7413793103448, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788660, Length=205, Percent_Identity=26.3414634146341, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI25147481, Length=278, Percent_Identity=29.136690647482, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17532191, Length=202, Percent_Identity=26.7326732673267, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=248, Percent_Identity=29.8387096774194, Blast_Score=116, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=288, Percent_Identity=29.1666666666667, Blast_Score=116, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6322116, Length=232, Percent_Identity=31.0344827586207, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=204, Percent_Identity=27.9411764705882, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324980, Length=140, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24646446, Length=258, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24646448, Length=258, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24646452, Length=258, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24646450, Length=258, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI62472511, Length=258, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19921140, Length=241, Percent_Identity=27.8008298755187, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45552429, Length=201, Percent_Identity=29.8507462686567, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI28574286, Length=238, Percent_Identity=27.7310924369748, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585514, Length=201, Percent_Identity=29.8507462686567, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28574282, Length=201, Percent_Identity=29.8507462686567, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28574284, Length=201, Percent_Identity=29.8507462686567, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI45551003, Length=201, Percent_Identity=29.8507462686567, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571528, Length=260, Percent_Identity=26.1538461538462, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585516, Length=199, Percent_Identity=26.6331658291457, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LDHD_ECOLI (P52643)

Other databases:

- EMBL:   U36928
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   G64888
- RefSeq:   AP_002007.1
- RefSeq:   NP_415898.1
- ProteinModelPortal:   P52643
- SMR:   P52643
- DIP:   DIP-10087N
- STRING:   P52643
- EnsemblBacteria:   EBESCT00000002021
- EnsemblBacteria:   EBESCT00000014636
- GeneID:   946315
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1375
- KEGG:   eco:b1380
- EchoBASE:   EB2978
- EcoGene:   EG13186
- eggNOG:   COG1052
- GeneTree:   EBGT00050000009079
- HOGENOM:   HBG731446
- OMA:   MAGFGCQ
- ProtClustDB:   CLSK869554
- BioCyc:   EcoCyc:DLACTDEHYDROGNAD-MONOMER
- BioCyc:   MetaCyc:DLACTDEHYDROGNAD-MONOMER
- Genevestigator:   P52643
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: =1.1.1.28

Molecular weight: Translated: 36535; Mature: 36535

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 234-234 ACT_SITE 263-263 ACT_SITE 295-295 BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPV
CEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHH
LEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRI
HHHHHHCCCEEEEEEECCCCCCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAMLRILKGFGMRLLAFDPYPSAA
HHHHHHHCCCCCEECCCCEEEEECCCCCEEECCHHHHHHHHHHHHCCCEEEEECCCCCHH
ALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAFEQMKNGVMIVNTSRGALIDS
HHHHCCEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEECH
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLT
HHHHHHHHCCCCCCCCCHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
AEALTSISQTTLQNLSNLEKGETCPNELV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANGCEAVCIFVNDDGSRPV
CEEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHH
LEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDPEAVAEHAIGMMMTLNRRI
HHHHHHCCCEEEEEEECCCCCCCCHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHH
HRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAMLRILKGFGMRLLAFDPYPSAA
HHHHHHHCCCCCEECCCCEEEEECCCCCEEECCHHHHHHHHHHHHCCCEEEEECCCCCHH
ALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEAAFEQMKNGVMIVNTSRGALIDS
HHHHCCEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEECH
QAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVIQDDVFRRLSACHNVLFTGHQAFLT
HHHHHHHHCCCCCCCCCHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
AEALTSISQTTLQNLSNLEKGETCPNELV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564