Click here to switch to the map view.

The map label for this gene is ydaO

Identifier: 218700064

GI number: 218700064

Start: 1768902

End: 1769837

Strand: Reverse

Name: ydaO

Synonym: ECIAI39_1695

Alternate gene names: 218700064

Gene position: 1769837-1768902 (Counterclockwise)

Preceding gene: 218700065

Following gene: 218700060

Centisome position: 34.49

GC content: 48.93

Gene sequence:

>936_bases
ATGTCGAAAAATCAAGAAATTAGTAAGAAAGAACAATACAATCTGAACAAATTACAAAAACGTCTGCGTCGTAACGTGGG
CGAAGCCATTGCTGACTTCAATATGATTGAAGAAGGCGATCGCATCATGGTTTGCCTCTCCGGGGGTAAAGACAGCTATA
CCATGCTGGAAATTCTGCGCAATTTGCAGCAAAGCGCGCCAATCAATTTTTCGCTGGTGGCTGTCAACCTCGATCAAAAA
CAACCGGGCTTCCCGGAACACGTTCTGCCCGAGTATCTTGAAAAGCTGGGCGTTGAGTACAAGATTGTGGAAGAGAACAC
TTACGGTATCGTGAAAGAGAAGATTCCGGAGGGCAAAACCACTTGCTCACTGTGTTCTCGTCTTCGTCGCGGTATCCTTT
ATCGCACCGCAACGGAACTGGGAGCGACGAAGATCGCGCTTGGTCACCATCGTGACGATATCCTGCAAACGCTGTTCTTA
AATATGTTCTACGGCGGCAAGATGAAAGGTATGCCTCCGAAACTGATGAGCGATGATGGCAAACATATCGTGATTCGTCC
GCTGGCCTACTGCCGCGAGAAAGATATTCAGCGCTTTGCCGATGCAAAAGCGTTCCCGATTATTCCGTGCAACCTGTGCG
GTTCACAGCCTAACCTGCAACGTCAGGTGATTGCTGACATGTTGCGTGACTGGGATAAACGTTATCCGGGGCGTATCGAG
ACGATGTTCAGCGCGATGCAGAATGTGGTGCCATCGCATCTGTGCGATACCAACCTGTTCGATTTCAAAGGCATTACCCA
CGGTTCTGAAGTGGTTAACCGGGGTGATCTGGCGTTTGATCGCGAAGAGATCCCTCTACAACCGGCGGGCTGGCAGCCTG
AAGAAGATGAAAATCAGTTGGATGAGTTACGACTGAATGTGGTTGAAGTGAAATAA

Upstream 100 bases:

>100_bases
ACCATACACGCCACAGTTTTTATATCAGATTTCTTATGCTGGGCGTTCCGGCATGACGCATACTCTTCTGATGCCATATA
ACGAATTGAGTCGCTTTTAA

Downstream 100 bases:

>100_bases
CCCGGATAGCGCCCGATGCGCAAGCTTATCGGGCTACTCTTATGGAGGCCGGAGAAGACGCGGCCAGCGTCGCATCCGGC
AATCCCGAAGAAGATATTGA

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSKNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQK
QPGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNRGDLAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK

Sequences:

>Translated_311_residues
MSKNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQK
QPGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNRGDLAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK
>Mature_310_residues
SKNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQ
PGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLN
MFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIET
MFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNRGDLAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=311, Percent_Identity=98.0707395498392, Blast_Score=635, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193211090, Length=260, Percent_Identity=30.7692307692308, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17540090, Length=235, Percent_Identity=27.2340425531915, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24651972, Length=234, Percent_Identity=26.9230769230769, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 35559; Mature: 35428

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILR
CCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
NLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKT
HHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHCCCCCH
TCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLNMFYGGKMKGMPPKLMSDDG
HHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
KHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
CEEEECCHHHHHHHHHHHHHCCCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNRGDLAFDREEIPLQPAGWQPEEDENQL
HHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
DELRLNVVEVK
HHHHHEEEECC
>Mature Secondary Structure 
SKNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILR
CCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
NLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKT
HHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHCCCCCH
TCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLNMFYGGKMKGMPPKLMSDDG
HHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
KHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIE
CEEEECCHHHHHHHHHHHHHCCCCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
TMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNRGDLAFDREEIPLQPAGWQPEEDENQL
HHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
DELRLNVVEVK
HHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA