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The map label for this gene is puuP

Identifier: 218700018

GI number: 218700018

Start: 1719531

End: 1720916

Strand: Reverse

Name: puuP

Synonym: ECIAI39_1647

Alternate gene names: 218700018

Gene position: 1720916-1719531 (Counterclockwise)

Preceding gene: 218700019

Following gene: 218700017

Centisome position: 33.53

GC content: 53.54

Gene sequence:

>1386_bases
ATGGCTATTAATTCACCACTGACTATTGCTGCGCAATCTGGCAAAACCCGTCTGCGAAAATCACTGAAATTGTGGCAGGT
GGTGATGATGGGTCTGGCCTATCTCACGCCGATGACCGTATTTGATACTTTTGGTATTGTCTCCGGCATTAGCGACGGTC
ACGTTCCGGCGTCCTATTTGCTGGCGCTGGCGGGCGTGCTGTTTACCGCTATCAGCTACGGCAAACTGGTTCGCCAGTTT
CCGGAAGCCGGTTCGGCCTATACCTACGCGCAAAAGTCGATTAACCCGCACGTCGGATTTATGGTCGGCTGGTCATCGCT
GCTGGATTATCTCTTTTTGCCGATGATCAACGTCTTGTTGGCGAAAATCTATCTCTCCGCCCTCTTCCCGGAAGTGCCGC
CGTGGGTGTGGGTGGTAACCTTCGTCGCCATTTTAACCGCCGCGAATCTGAAGAGCGTCAACCTGGTTGCTAACTTCAAT
ACCCTGTTTGTGTTGGTGCAAATCTCCATCATGGTGGTGTTTATCTTCCTGGTGGTTCAGGGACTGCATAAAGGAGAAGG
CGTTGGCACCGTCTGGTCACTTCAGCCGTTTATCAGCGAGAACGTGCACCTGATCCCGATTATTACCGGGGCGACGATTG
TCTGTTTCTCCTTCCTCGGCTTCGATGCGGTGACCACGCTTTCGGAAGAGACGCCGGACGCCGCACGCGTGATCCCGAAA
GCCATCTTCCTGACGGCGGTCTACGGTGGCGTTATCTTTATCGCTGCGTCGTTCTTTATGCAGCTGTTCTTTCCTGATAT
CAGCCGCTTTAAAGACCCGGACGCCGCACTGCCTGAAATTGCGCTGTACGTCGGCGGCAAGCTCTTCCAGTCGATTTTCC
TCTGTACCACGTTTGTGAACACGTTAGCGTCTGGCCTGGCGTCGCACGCCAGCGTGTCGCGTCTGCTGTATGTGATGGGA
CGTGACAATGTGTTTCCGGAGCGCGTGTTTGGCTATGTGCACCCGAAATGGCGCACGCCAGCGTTGAACGTCATTATGGT
CGGGATTGTTGCGCTGTCGGCGCTGTTCTTTGATTTAGTCACCGCGACAGCATTGATTAACTTCGGTGCTCTGGTGGCGT
TTACCTTCGTAAATCTGTCGGTGTTTAATCATTTCTGGCGGCGCAAAGGAATGAATAAAAGCTGGAAGGATCACTTCCAC
TATTTGCTGATGCCGCTGGTTGGCGCGCTGACGGTGGGTGTGCTGTGGGTTAACCTCGAGTCAACGTCACTGACACTCGG
TCTGGTATGGGCTTCGTTAGGCGGCGCATATTTGTGGTATCTGATCCGCCGCTATCGCAAAGTGCCGTTGTATGATGGTG
ACAGAACGCCGTTGAGCGAAACGTAA

Upstream 100 bases:

>100_bases
GCGACTGACTGGACTCCGCCCCCTTTGCTTCCCTTGCTAAACAGGTTTGATTTCAGGTCGACACGACCGCAAACCTTATT
ACGCAGGGGAGGCAGCAACT

Downstream 100 bases:

>100_bases
TAGTCGGATGCGCGGCGCATTATCCTGCCTGAGGGTAATGCACCGCGCCATTAACTATAAAAAGATGTATGACCCGGATC
ACGAGTGCTATCATCCGCCC

Product: putrescine importer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MAINSPLTIAAQSGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQF
PEAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENVHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMG
RDNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFH
YLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPLYDGDRTPLSET

Sequences:

>Translated_461_residues
MAINSPLTIAAQSGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQF
PEAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENVHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMG
RDNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFH
YLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPLYDGDRTPLSET
>Mature_460_residues
AINSPLTIAAQSGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFP
EAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFNT
LFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENVHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPKA
IFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGR
DNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFHY
LLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPLYDGDRTPLSET

Specific function: Imports putrescine [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Homo sapiens, GI114326544, Length=432, Percent_Identity=22.6851851851852, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI114326550, Length=432, Percent_Identity=22.6851851851852, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI4507047, Length=417, Percent_Identity=22.7817745803357, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI87081869, Length=461, Percent_Identity=99.1323210412148, Blast_Score=920, Evalue=0.0,
Organism=Escherichia coli, GI87082023, Length=446, Percent_Identity=63.2286995515695, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1788480, Length=395, Percent_Identity=24.0506329113924, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081708, Length=467, Percent_Identity=24.4111349036403, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786789, Length=175, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786602, Length=176, Percent_Identity=25.5681818181818, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87082250, Length=403, Percent_Identity=22.8287841191067, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786302, Length=245, Percent_Identity=23.265306122449, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17532491, Length=400, Percent_Identity=23.75, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17533459, Length=404, Percent_Identity=24.5049504950495, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17531343, Length=459, Percent_Identity=24.6187363834423, Blast_Score=65, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24667468, Length=406, Percent_Identity=26.1083743842365, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24668806, Length=428, Percent_Identity=24.7663551401869, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356285, Length=428, Percent_Identity=24.7663551401869, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24668802, Length=428, Percent_Identity=24.7663551401869, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281366235, Length=411, Percent_Identity=24.5742092457421, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI116007820, Length=411, Percent_Identity=24.5742092457421, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI116007818, Length=413, Percent_Identity=24.9394673123487, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI85725152, Length=413, Percent_Identity=24.9394673123487, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24666159, Length=399, Percent_Identity=21.0526315789474, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221512776, Length=399, Percent_Identity=21.0526315789474, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 50873; Mature: 50741

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAINSPLTIAAQSGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYL
CCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFNTLFVLVQISIMVVFIFLVVQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
GLHKGEGVGTVWSLQPFISENVHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
HHCCCCCCCEEEECCHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLASGLASHASVSRLLYVMGRDNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFHYLLMPLVGALTVGVLWVNLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEC
STSLTLGLVWASLGGAYLWYLIRRYRKVPLYDGDRTPLSET
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AINSPLTIAAQSGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGISDGHVPASYL
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMVGWSSLLDYLFLPMINVLL
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFNTLFVLVQISIMVVFIFLVVQ
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
GLHKGEGVGTVWSLQPFISENVHLIPIITGATIVCFSFLGFDAVTTLSEETPDAARVIPK
HHCCCCCCCEEEECCHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAALPEIALYVGGKLFQSIFLCTTFVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TLASGLASHASVSRLLYVMGRDNVFPERVFGYVHPKWRTPALNVIMVGIVALSALFFDLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TATALINFGALVAFTFVNLSVFNHFWRRKGMNKSWKDHFHYLLMPLVGALTVGVLWVNLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEC
STSLTLGLVWASLGGAYLWYLIRRYRKVPLYDGDRTPLSET
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]