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The map label for this gene is sapA

Identifier: 218700016

GI number: 218700016

Start: 1717196

End: 1718839

Strand: Reverse

Name: sapA

Synonym: ECIAI39_1645

Alternate gene names: 218700016

Gene position: 1718839-1717196 (Counterclockwise)

Preceding gene: 218700017

Following gene: 218700015

Centisome position: 33.49

GC content: 54.14

Gene sequence:

>1644_bases
ATGCGCCAGGTATTATCGTCTCTTTTGGTGATTGCTGGACTTGTGAGTGGTCAGGCAATCGCCGCGCCTGAATCCCCCCC
GCATGCTGATATCCGCGACAGCGGTTTTGTCTATTGCGTCAGCGGGCAAGTCAACACCTTTAACCCATCCAAAGCGAGCA
GTGGGTTAATTGTCGATACCCTTGCCGCCCAGTTTTATGATCGACTGCTGGATGTCGATCCCTATACCTATCGCCTGATG
CCGGAACTTGCCGAAAGCTGGGAAGTACTCGACAACGGCGCGACCTATCGCTTCCACCTGCGTCGCGATGTTCCGTTTCA
AAAAACCGACTGGTTTACTCCCACTCGTAAAATGAATGCCGACGATGTGGTGTTTACCTTCCAGCGAATTTTTGACCGCA
ACAACCCGTGGCATAACGTTAATGGCAGCAACTTCCCCTATTTCGACAGCCTGCAATTTGCCGATAACGTGAAAAGCGTC
CGCAAACTGGATAATCATACCGTTGAGTTCCGTCTGGCTCAGCCGGATGCTTCTTTTTTGTGGCACCTCGCGACCCATTA
TGCTTCGGTCATATCGGCAGAATATGCAGGGAAGTTAGAGAAAGAGGATCGCCAGGAGCAACTCGACCGTCAGCCGGTCG
GCACTGGACCGTATCAGTTGTCGGAATACCGCGCCGGGCAATTTATTCGCCTACAACGTCATGATGGCTTCTGGCGCGGC
AAACCGTTAATGCCGCAGGTGGTGGTGGATTTAGGCTCCGGCGGTACCGGACGTCTGTCGAAACTCCTGACCGGGGAATG
CGACGTTCTGGCCTGGCCTGCCGCCAGTCAGCTATCCATTTTGCGTGACGACCCGCGCTTGCGTTTAACGCTGCGCCCGG
GGATGAACGTCGCCTATCTGGCGTTTAACACCGCTAAACCGCCGCTAAATAATCCGGCGGTCCGCCATGCACTGGCACTG
GCGATTAATAACCAGCGCCTGATGCAATCCATCTATTATGGTACGGCTGAAACGGCGGCCTCTATTTTACCGCGCGCCTC
GTGGGCCTATGACAACGAGGCTAAAATTACTGAATACAATCCGGCAAAATCGCGCGAACAGTTGAAGGCGTTGGGGCTGG
AAAACTTAACGCTGAAACTGTGGGTGCCCACACGTTCGCAGGCGTGGAACCCCAGTCCACTGAAAACCGCCGAACTGATT
CAGGCGGATATGGCACAGGTTGGCGTAAAAGTGGTGATTGTGCCGGTAGAAGGTCGTTTTCAGGAGGCGCGGTTGATGGA
TATGAGCCATGATCTGACGTTATCCGGTTGGGCGACGGACAGTAACGACCCGGACAGTTTCTTCCGTCCGTTACTGAGCT
GTGCGGCAATTCATTCACAGACCAACCTCGCCCACTGGTGCGATCCGAAATTCGACAGCGTATTGCGTAAGGCGCTCTCT
TCGCAGCAGCTGGCGGCGCGTATTGAAGCCTATGACGAAGCGCAAAGTATTCTGGCGCAGGAATTACCTATTCTGCCGCT
GGCGTCGTCATTGCGTTTGCAGGCCTATCGGTACGATATCAAAGGTCTGGTACTTAGCCCGTTTGGTAACGCCTCCTTTG
CTGGGGTGTATCGCGAGAAACAGGATGAGGTGAAAAAGCCATGA

Upstream 100 bases:

>100_bases
AACCACACGGCCCTCATTACGACCCCTGAACACAAACGGTAATGGCAAATGTCATTAAATATTAGTAAGATAGCCTCTTT
ACATAAAATCCATTAATATT

Downstream 100 bases:

>100_bases
TTATCTTCACCTTACGCCGTATTTTGTTATTGATTGTCACCTTGTTCCTGCTGACCTTCGTTGGCTTCAGCTTGAGCTAT
TTCACCCCACATGCGCCGTT

Product: antimicrobial peptide ABC transporter periplasmic-binding protein

Products: ADP; phosphate; peptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLM
PELAESWEVLDNGATYRFHLRRDVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
RKLDNHTVEFRLAQPDASFLWHLATHYASVISAEYAGKLEKEDRQEQLDRQPVGTGPYQLSEYRAGQFIRLQRHDGFWRG
KPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALAL
AINNQRLMQSIYYGTAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPSPLKTAELI
QADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRPLLSCAAIHSQTNLAHWCDPKFDSVLRKALS
SQQLAARIEAYDEAQSILAQELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP

Sequences:

>Translated_547_residues
MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLM
PELAESWEVLDNGATYRFHLRRDVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
RKLDNHTVEFRLAQPDASFLWHLATHYASVISAEYAGKLEKEDRQEQLDRQPVGTGPYQLSEYRAGQFIRLQRHDGFWRG
KPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALAL
AINNQRLMQSIYYGTAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPSPLKTAELI
QADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRPLLSCAAIHSQTNLAHWCDPKFDSVLRKALS
SQQLAARIEAYDEAQSILAQELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP
>Mature_547_residues
MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLM
PELAESWEVLDNGATYRFHLRRDVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
RKLDNHTVEFRLAQPDASFLWHLATHYASVISAEYAGKLEKEDRQEQLDRQPVGTGPYQLSEYRAGQFIRLQRHDGFWRG
KPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALAL
AINNQRLMQSIYYGTAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPSPLKTAELI
QADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRPLLSCAAIHSQTNLAHWCDPKFDSVLRKALS
SQQLAARIEAYDEAQSILAQELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP

Specific function: Involved in a peptide intake transport system that plays a role in the resistance to antimicrobial peptides [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787551, Length=547, Percent_Identity=99.2687385740402, Blast_Score=1122, Evalue=0.0,
Organism=Escherichia coli, GI1789966, Length=512, Percent_Identity=35.9375, Blast_Score=378, Evalue=1e-106,
Organism=Escherichia coli, GI1787052, Length=546, Percent_Identity=27.4725274725275, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1787762, Length=467, Percent_Identity=23.3404710920771, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1787495, Length=537, Percent_Identity=24.3947858472998, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1789887, Length=496, Percent_Identity=25, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1789397, Length=523, Percent_Identity=23.3269598470363, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61374; Mature: 61374

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTFNPSKASSGLIVDT
CHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHH
LAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRRDVPFQKTDWFTPTRKMNA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
DDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSVRKLDNHTVEFRLAQPDASFL
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHH
WHLATHYASVISAEYAGKLEKEDRQEQLDRQPVGTGPYQLSEYRAGQFIRLQRHDGFWRG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEECCCCCCCC
KPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLSILRDDPRLRLTLRPGMNVAYL
CCCCHHHEEECCCCCCHHHHHHHCCCCCEEECCCCCCHHEEECCCCEEEEECCCCCEEEE
AFNTAKPPLNNPAVRHALALAINNQRLMQSIYYGTAETAASILPRASWAYDNEAKITEYN
EEECCCCCCCCHHHHHHHHHEECCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCEEECC
PAKSREQLKALGLENLTLKLWVPTRSQAWNPSPLKTAELIQADMAQVGVKVVIVPVEGRF
CCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCC
QEARLMDMSHDLTLSGWATDSNDPDSFFRPLLSCAAIHSQTNLAHWCDPKFDSVLRKALS
CHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
SQQLAARIEAYDEAQSILAQELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEEEECEEEECCCCCCCCHHHHHHH
QDEVKKP
HHHCCCC
>Mature Secondary Structure
MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQVNTFNPSKASSGLIVDT
CHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHH
LAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRRDVPFQKTDWFTPTRKMNA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCC
DDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSVRKLDNHTVEFRLAQPDASFL
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHH
WHLATHYASVISAEYAGKLEKEDRQEQLDRQPVGTGPYQLSEYRAGQFIRLQRHDGFWRG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHHCCCCCEEEEECCCCCCCC
KPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAASQLSILRDDPRLRLTLRPGMNVAYL
CCCCHHHEEECCCCCCHHHHHHHCCCCCEEECCCCCCHHEEECCCCEEEEECCCCCEEEE
AFNTAKPPLNNPAVRHALALAINNQRLMQSIYYGTAETAASILPRASWAYDNEAKITEYN
EEECCCCCCCCHHHHHHHHHEECCHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCEEECC
PAKSREQLKALGLENLTLKLWVPTRSQAWNPSPLKTAELIQADMAQVGVKVVIVPVEGRF
CCHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCC
QEARLMDMSHDLTLSGWATDSNDPDSFFRPLLSCAAIHSQTNLAHWCDPKFDSVLRKALS
CHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHH
SQQLAARIEAYDEAQSILAQELPILPLASSLRLQAYRYDIKGLVLSPFGNASFAGVYREK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEEEEEECEEEECCCCCCCCHHHHHHH
QDEVKKP
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; peptides [Periplasm]; H2O [C]

Specific reaction: ATP + peptides [Periplasm] + H2O = ADP + phosphate + peptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8223423; 11677609 [H]