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The map label for this gene is trpE

Identifier: 218699971

GI number: 218699971

Start: 1670807

End: 1672369

Strand: Reverse

Name: trpE

Synonym: ECIAI39_1601

Alternate gene names: 218699971

Gene position: 1672369-1670807 (Counterclockwise)

Preceding gene: 218699972

Following gene: 218699970

Centisome position: 32.59

GC content: 53.74

Gene sequence:

>1563_bases
ATGCAAACACAAAAACCGACTCTCGAACTGCTAACTTGCAAAGGCGCTTATCGCGACAACCCGACTGCGCTTTTTCACCA
GTTGTGTGGGGATCGTCCGGCAACGCTGCTGCTGGAATCCGCAGATATCGACAGCAAAGATGATTTAAAAAGTCTATTGC
TAGTGGACAGTGCGCTGCGCATTACAGCATTAGGTGACACTGTCACTATTCAGGCACTTTCCGGCAATGGCGAAGCCCTG
TTGGCATTACTGGATAACGCCCTGCCTGCGGGTGTGGAAAATGAACAATCACCAAACTGCCGCGTGCTGCGCTTCCCTCC
TGTCAGTCCATTGCTGGATGAAGACGCCCGCTTATGCTCCCTTTCGGTTTTTGACGCTTTCCGCATATTGCAGAATCTGT
TGAATGTACCGAAGGAAGAACGAGAAGCAATGTTCTTCGGCGGCCTGTTCTCTTATGACCTTGTGGCGGGGTTTGAAGAT
TTACCGCAATTGTCAGCGGAAAATAACTGCCCTGATTTCTGTTTTTATCTCGCTGAAACGCTGATGGTGATTGACCATCA
GAAGAAAAGCACCCGCATTCAGGCCAGCCTGTTTACTCCAAATGAAGAAGAAAAACAACGTCTCACTGCTCGCCTGAACG
AACTTCGCCAGCAACTGACCGAAACCGCGCCACCGCTGCCGGTAGTTTCCGTGCCGCATATGCTTTGTGAATGTAATCAG
AGCGATGAAGAGTTCGGTGGCGTAGTGCGTTCGTTGCAAAAAGCGATTCGCGCCGGAGAAATTTTCCAGGTGGTGCCATC
TCGCCGTTTCTCTCTGCCCTGCCCGTCACCACTGGCGGCCTATTACGTGCTGAAAAAGAGTAATCCCAGCCCGTACATGT
TTTTTATGCAGGATAATGATTTCACCCTGTTTGGCGCGTCGCCGGAAAGTTCGCTCAAGTATGACGCCACCAGCCGCCAG
ATTGAGATTTACCCGATTGCCGGAACACGTCCACGCGGTCGTCGTGCCGATGGTTCACTGGACAGAGACCTCGACAGCCG
CATCGAACTGGAAATGCGTACCGATCATAAAGAGCTTTCTGAACATCTGATGCTGGTGGATCTCGCCCGTAATGATCTGG
CACGCATTTGCACGCCCGGCAGCCGCTACGTCGCCGATCTTACCAAAGTTGACCGTTACTCTTACGTGATGCACCTGGTC
TCCCGCGTGGTCGGTGAGCTGCGCCACGATCTCGACGCCCTGCACGCTTACCGCGCCTGTATGAATATGGGGACGTTAAG
CGGTGCGCCGAAAGTACGCGCTATGCAGTTAATTGCCGAAGCAGAAGGTCGTCGCCGCGGCAGCTACGGCGGCGCGGTAG
GTTATTTCACCGCAAATGGCGATCTCGACACCTGCATTGTGATCCGCTCGGCGCTCGTGGAAAACGGTATCGCCACCGTG
CAAGCCGGTGCTGGCGTAGTCCTTGATTCTGTTCCGCAGTCGGAAGCCGACGAAACCCGTAATAAAGCCCGCGCTGTACT
GCGCGCTATTGCCACCGCGCATCATGCACAGGAGACTTTCTGA

Upstream 100 bases:

>100_bases
ACTTCCTGAAACGGGCAGTGTATTCACCATGCGTAAAGCAATCAGATACCCAGCCCGCCTAATGAGCGGGCTTTTTTTTG
AACAAAATTAGAGAATAACA

Downstream 100 bases:

>100_bases
TGGCTGACATTCTGCTACTCGATAATATCGACTCTTTTACGTACAACCTGGCAGATCAGTTGCGCAGCAATGGTCATAAC
GTGGTGATTTACCGCAACCA

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MQTQKPTLELLTCKGAYRDNPTALFHQLCGDRPATLLLESADIDSKDDLKSLLLVDSALRITALGDTVTIQALSGNGEAL
LALLDNALPAGVENEQSPNCRVLRFPPVSPLLDEDARLCSLSVFDAFRILQNLLNVPKEEREAMFFGGLFSYDLVAGFED
LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFTPNEEEKQRLTARLNELRQQLTETAPPLPVVSVPHMLCECNQ
SDEEFGGVVRSLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQ
IEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLV
SRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTANGDLDTCIVIRSALVENGIATV
QAGAGVVLDSVPQSEADETRNKARAVLRAIATAHHAQETF

Sequences:

>Translated_520_residues
MQTQKPTLELLTCKGAYRDNPTALFHQLCGDRPATLLLESADIDSKDDLKSLLLVDSALRITALGDTVTIQALSGNGEAL
LALLDNALPAGVENEQSPNCRVLRFPPVSPLLDEDARLCSLSVFDAFRILQNLLNVPKEEREAMFFGGLFSYDLVAGFED
LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFTPNEEEKQRLTARLNELRQQLTETAPPLPVVSVPHMLCECNQ
SDEEFGGVVRSLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQ
IEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLV
SRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTANGDLDTCIVIRSALVENGIATV
QAGAGVVLDSVPQSEADETRNKARAVLRAIATAHHAQETF
>Mature_520_residues
MQTQKPTLELLTCKGAYRDNPTALFHQLCGDRPATLLLESADIDSKDDLKSLLLVDSALRITALGDTVTIQALSGNGEAL
LALLDNALPAGVENEQSPNCRVLRFPPVSPLLDEDARLCSLSVFDAFRILQNLLNVPKEEREAMFFGGLFSYDLVAGFED
LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFTPNEEEKQRLTARLNELRQQLTETAPPLPVVSVPHMLCECNQ
SDEEFGGVVRSLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQ
IEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLV
SRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTANGDLDTCIVIRSALVENGIATV
QAGAGVVLDSVPQSEADETRNKARAVLRAIATAHHAQETF

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=520, Percent_Identity=98.4615384615385, Blast_Score=1050, Evalue=0.0,
Organism=Escherichia coli, GI1788114, Length=422, Percent_Identity=29.6208530805687, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1786809, Length=227, Percent_Identity=29.0748898678414, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI87082077, Length=270, Percent_Identity=28.8888888888889, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320935, Length=399, Percent_Identity=37.0927318295739, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6324361, Length=444, Percent_Identity=24.0990990990991, Blast_Score=88, Evalue=4e-18,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005257
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 57489; Mature: 57489

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTQKPTLELLTCKGAYRDNPTALFHQLCGDRPATLLLESADIDSKDDLKSLLLVDSALR
CCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCEE
ITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRVLRFPPVSPLLDEDARLCS
EEEECCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHH
LSVFDAFRILQNLLNVPKEEREAMFFGGLFSYDLVAGFEDLPQLSAENNCPDFCFYLAET
HHHHHHHHHHHHHHCCCHHHHHHEEECCHHHHHHHHCHHHHHHCCCCCCCHHHHHHHHHH
LMVIDHQKKSTRIQASLFTPNEEEKQRLTARLNELRQQLTETAPPLPVVSVPHMLCECNQ
HHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC
SDEEFGGVVRSLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDND
CHHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCCCCHHHEEEEEECCCCCCEEEEEECCC
FTLFGASPESSLKYDATSRQIEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELS
EEEEECCCCCCCEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHH
EHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLVSRVVGELRHDLDALHAYRAC
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTANGDLDTCIVIRSALVENGIATV
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEE
QAGAGVVLDSVPQSEADETRNKARAVLRAIATAHHAQETF
ECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQTQKPTLELLTCKGAYRDNPTALFHQLCGDRPATLLLESADIDSKDDLKSLLLVDSALR
CCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCEE
ITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRVLRFPPVSPLLDEDARLCS
EEEECCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHH
LSVFDAFRILQNLLNVPKEEREAMFFGGLFSYDLVAGFEDLPQLSAENNCPDFCFYLAET
HHHHHHHHHHHHHHCCCHHHHHHEEECCHHHHHHHHCHHHHHHCCCCCCCHHHHHHHHHH
LMVIDHQKKSTRIQASLFTPNEEEKQRLTARLNELRQQLTETAPPLPVVSVPHMLCECNQ
HHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCC
SDEEFGGVVRSLQKAIRAGEIFQVVPSRRFSLPCPSPLAAYYVLKKSNPSPYMFFMQDND
CHHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCCCCHHHEEEEEECCCCCCEEEEEECCC
FTLFGASPESSLKYDATSRQIEIYPIAGTRPRGRRADGSLDRDLDSRIELEMRTDHKELS
EEEEECCCCCCCEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHH
EHLMLVDLARNDLARICTPGSRYVADLTKVDRYSYVMHLVSRVVGELRHDLDALHAYRAC
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MNMGTLSGAPKVRAMQLIAEAEGRRRGSYGGAVGYFTANGDLDTCIVIRSALVENGIATV
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCEE
QAGAGVVLDSVPQSEADETRNKARAVLRAIATAHHAQETF
ECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7038627; 7021857; 9097039; 9278503; 351195; 4598537 [H]