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The map label for this gene is narX

Identifier: 218699931

GI number: 218699931

Start: 1626844

End: 1628640

Strand: Reverse

Name: narX

Synonym: ECIAI39_1560

Alternate gene names: 218699931

Gene position: 1628640-1626844 (Counterclockwise)

Preceding gene: 218699938

Following gene: 218699930

Centisome position: 31.73

GC content: 53.26

Gene sequence:

>1797_bases
ATGCTTAAACGTTGTCTCTCTCCGCTCACCCTGGTTAATCAGGTTGCGCTTATTGTGTTGCTTTCTACTGCTATTGGACT
GGCAGGGATGGCAGTTTCTGGCTGGCTGGTGCAAGGCGTTCAGGGCAGCGCCCATGCGATCAACAAAGCGGGATCGCTGC
GCATGCAAAGTTACCGTCTGTTGGCGGCAGTGCCATTAAGCGAGAAAGACAAGCCCTTAATTAAAGAAATGGAACAAACG
GCATTTAGCGCCGAGTTGACTCGAGCAGCGGAACGAGACGGACAACTGGCGCAATTACAGGGTTTACAAGATTACTGGCG
TAATGAACTGATCCCTGCGCTGATGCGTGCACAAAACCGCGAAACGGTGTCAGCGGATGTCAGCCAGTTTGTTGCCGGGC
TTGATCAGCTGGTATCTGGTTTTGACCGCACCACGGAAATGCGCATTGAGACAGTGGTACTGGTCCATCGGGTAATGGCG
GTATTTATGGCACTTTTACTGGTGTTCACTATTATCTGGTTGCGGGCGCGACTGCTACAACCGTGGCGGCAACTGCTGGC
AATGGCGAGTGCCGTCAGCCATCGCGATTTTACCCAACGCGCAAACATCAGCGGGCGCAACGAAATGGCGATGCTTGGAA
CTGCATTGAACAATATGTCTGCAGAACTGGCCGAAAGTTATGCCGTACTTGAGCAGCGGGTTCAGGAGAAAACTGCCGGG
CTGGAGCATAAAAATCAGATCCTCTCTTTTTTATGGAAGGCTAACCGCCGTTTGCATTCCCGCGCCCCGCTGTGTGAACG
CCTGTCACCGGTACTCAACGGCTTACAGAATTTAACCCTGCTACGTGATATCGAACTGCGGGTGTATGACACTGATGATG
AAGAGAATCATCAGGAGTTTACCTGCCAGCCAGATATGACTTGTGATGATAAAGGCTGCCAGCTCTGCCCGCGCGGCATA
TTACCCGTTGGCGATCGCGGCACAACCCTGAAGTGGCGGCTGGCTGACTCTCATACGCAGTACGGTATTTTGCTGGCGAC
CCTGCCGCAGGGGCGTCATCTTAGCCATGATCAACAACAACTGGTGGATACCCTGGTTGAACAACTCACCGCCACGCTGG
CGCTGGATCGCCATCAGGAACGTCAGCAACAGTTGATCGTGATGGAAGAGCGTGCCACCATTGCGCGCGAACTGCATGAT
TCTATTGCCCAATCTCTCTCTTGCATGAAGATGCAGGTGAGTTGTTTACAGATGCAGGGCGATGCGCTGCCAGAAAGCAG
CCGCGAACTGTTAAGTCAGATCCGTAACGAACTGAATGCATCCTGGGCGCAGTTGCGTGAATTGCTCACCACATTCCGTT
TGCAGCTCACCGAGCCTGGATTACGTCCGGCGCTGGAGGCGAGTTGCGAAGAGTACAGCGCCAAATTTGGCTTCCCGGTG
AAGCTGGATTATCAATTGCCGCCTCGTCTGGTGCCTTCGCATCAGGCAATCCACTTGTTGCAAATTGCCCGTGAGGCATT
AAGTAACGCCCTCAAACATTCGCAAGCGAGTGAGGTCGTGGTGACGGTGGCGCAAAACGATAATCAGGTCAAACTGACCG
TCCAGGATAACGGCTGCGGCGTGCCTGAAAATGCCATCCGCAGCAATCACTACGGCATGATAATAATGCGCGACCGTGCG
CAAAGTTTACGAGGCGATTGCCGCGTCCGCCGTCGTGAATCAGGTGGCACCGAAGTGGTTGTCACCTTTATTCCCGAAAA
AACTTTCACAGACGTCCAAGGAGATACCCATGAGTAA

Upstream 100 bases:

>100_bases
GGTATACTTCAGCCAATAGCCGAGAATACTGCCATTCCAGAATGTATCGTCACATTCATTAAGGTTATTGCTCATTTAAA
GCCTGAAGGAAGTGGTTTAC

Downstream 100 bases:

>100_bases
TCAGGAACCGGCTACTATCCTGCTGATCGACGATCACCCGATGCTGCGAACTGGCGTAAAACAGCTTATCAGTATGGCAC
CAGATATCACCGTGGTTGGC

Product: nitrate/nitrite sensor protein NarX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQT
AFSAELTRAAERDGQLAQLQGLQDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNNMSAELAESYAVLEQRVQEKTAG
LEHKNQILSFLWKANRRLHSRAPLCERLSPVLNGLQNLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGI
LPVGDRGTTLKWRLADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLTTFRLQLTEPGLRPALEASCEEYSAKFGFPV
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRA
QSLRGDCRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE

Sequences:

>Translated_598_residues
MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQT
AFSAELTRAAERDGQLAQLQGLQDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNNMSAELAESYAVLEQRVQEKTAG
LEHKNQILSFLWKANRRLHSRAPLCERLSPVLNGLQNLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGI
LPVGDRGTTLKWRLADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLTTFRLQLTEPGLRPALEASCEEYSAKFGFPV
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRA
QSLRGDCRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE
>Mature_598_residues
MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQT
AFSAELTRAAERDGQLAQLQGLQDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNNMSAELAESYAVLEQRVQEKTAG
LEHKNQILSFLWKANRRLHSRAPLCERLSPVLNGLQNLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGI
LPVGDRGTTLKWRLADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLTTFRLQLTEPGLRPALEASCEEYSAKFGFPV
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRA
QSLRGDCRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the narL protein and of both nitrate/nitrite to the narP protein. NarX probably activates narL and narP by phosphorylation in the presence of nitrate. NarX also plays a

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=598, Percent_Identity=99.6655518394649, Blast_Score=1225, Evalue=0.0,
Organism=Escherichia coli, GI1788812, Length=588, Percent_Identity=29.421768707483, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI87082311, Length=218, Percent_Identity=27.5229357798165, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR003661
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 67099; Mature: 67099

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHE
LAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGLQDYWRNELIPALMRAQNR
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
ETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMAVFMALLLVFTIIWLRARLLQ
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNNMSAELAESYAVLEQRVQEKTAG
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LEHKNQILSFLWKANRRLHSRAPLCERLSPVLNGLQNLTLLRDIELRVYDTDDEENHQEF
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCE
TCQPDMTCDDKGCQLCPRGILPVGDRGTTLKWRLADSHTQYGILLATLPQGRHLSHDQQQ
ECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCHHHH
LVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHDSIAQSLSCMKMQVSCLQMQG
HHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DALPESSRELLSQIRNELNASWAQLRELLTTFRLQLTEPGLRPALEASCEEYSAKFGFPV
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCE
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSQASEVVVTVAQNDNQVKLTVQDNGCG
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCC
VPENAIRSNHYGMIIMRDRAQSLRGDCRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE
CCHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHE
LAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGLQDYWRNELIPALMRAQNR
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
ETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMAVFMALLLVFTIIWLRARLLQ
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNNMSAELAESYAVLEQRVQEKTAG
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LEHKNQILSFLWKANRRLHSRAPLCERLSPVLNGLQNLTLLRDIELRVYDTDDEENHQEF
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCE
TCQPDMTCDDKGCQLCPRGILPVGDRGTTLKWRLADSHTQYGILLATLPQGRHLSHDQQQ
ECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCHHHH
LVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHDSIAQSLSCMKMQVSCLQMQG
HHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DALPESSRELLSQIRNELNASWAQLRELLTTFRLQLTEPGLRPALEASCEEYSAKFGFPV
CCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCE
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSQASEVVVTVAQNDNQVKLTVQDNGCG
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCC
VPENAIRSNHYGMIIMRDRAQSLRGDCRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE
CCHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]