Click here to switch to the map view.

The map label for this gene is ldcA

Identifier: 218699894

GI number: 218699894

Start: 1590943

End: 1591857

Strand: Reverse

Name: ldcA

Synonym: ECIAI39_1523

Alternate gene names: 218699894

Gene position: 1591857-1590943 (Counterclockwise)

Preceding gene: 218699896

Following gene: 218699893

Centisome position: 31.02

GC content: 52.68

Gene sequence:

>915_bases
ATGTCTCTGTTTCACTTAATTGCCCCATCGGGTTACTGCATTAAACAGCACGCCGCGCTGCGTGGTATCCAGCGCCTTAC
CGACGCGGGGCATCAGGTCAATAACGTCGAGGTTATTGCCCGTCGTTGTGAGCGTTTTGCGGGTACGGAAACGGAGCGTC
TGGAAGATCTCAATTCTCTTGCCAGACTGACTACCCCCAACACCATCGTGCTGGCTGTACGCGGCGGTTACGGTGCCAGT
CGTTTACTGGCAGATATTGACTGGCAGGCACTGGTGGCTCGCCAACAACATGACCCGTTGCTCATTTGCGGACATAGCGA
TTTTACCGCCATTCAGTGCGGTCTTCTGGCGCATGGCAATGTCATCACCTTTAGCGGCCCCATGCTGGTAGCAAACTTTG
GCGCGGATGAACTGAACGCCTTTACCGAGCACCATTTCTGGCTGGCGTTACGCAATGAAACCTTCACCATTGAATGGCAA
GGGGAAGGCCCGACATGTCAGACCGAAGGCACGCTGTGGGGAGGCAATCTCGCGATGCTGATTTCACTGATTGGTACACC
GTGGATGCCGCAAATCGAAAACGGTATTCTGGTGCTGGAAGATATTAACGAGCATCCTTTCCGCGTCGAACGTATGCTGT
TGCAGCTCTATCATGCTGGAATTTTGCCACGTCAGAAGGCGATTATTCTCGGTAGCTTTAGCGGCAACACGCCCAATGAT
TATGACGCAGGTTACAACCTGGAGTCAGTGTACGCGTTTTTGCGTTCCCGCCTGTCGATTCCACTCATTACCGGCCTCGA
TTTTGGTCATGAACAACGTACGGTTACGCTGCCGTTGGGCGCACAAGCCATGCTTAATAACACCCAGGAAGGCACTCAGT
TGACGATTAGCGGTCATCCTGTTCTTAAAATGTAA

Upstream 100 bases:

>100_bases
CTTCTTCTATCCTTATAGCCTGGAATCGCCGTTGAGGTGACAACGGCACACCTAAGCGGCATGATATACATTTCGTTTCT
GTTGTCAGCAAGGAATTGCC

Downstream 100 bases:

>100_bases
AAAAATCACGCTTCAGGGCTAAGTAAAACGCTGTCTCTGCCGCTAATATGGTAGGGTTTATATAAGAGACAGCGTAATCA
GGAGTAACCGACCGTTGGAT

Product: L,D-carboxypeptidase A

Products: NA

Alternate protein names: LD-carboxypeptidase A; Muramoyltetrapeptide carboxypeptidase [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGAS
RLLADIDWQALVARQQHDPLLICGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
GEGPTCQTEGTLWGGNLAMLISLIGTPWMPQIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGNTPND
YDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAQAMLNNTQEGTQLTISGHPVLKM

Sequences:

>Translated_304_residues
MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGAS
RLLADIDWQALVARQQHDPLLICGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
GEGPTCQTEGTLWGGNLAMLISLIGTPWMPQIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGNTPND
YDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAQAMLNNTQEGTQLTISGHPVLKM
>Mature_303_residues
SLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASR
LLADIDWQALVARQQHDPLLICGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQG
EGPTCQTEGTLWGGNLAMLISLIGTPWMPQIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGNTPNDY
DAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAQAMLNNTQEGTQLTISGHPVLKM

Specific function: Releases the terminal D-alanine residue from the cytoplasmic tetrapeptide recycling product L-Ala-gamma-D-Glu-meso- Dap-D-Ala. Can also cleave D-Ala from murein derivatives containing the tetrapeptide, i.e. MurNAc-tetrapeptide, UDP-MurNAc- tetrapeptide, G

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=304, Percent_Identity=97.6973684210526, Blast_Score=614, Evalue=1e-177,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 33578; Mature: 33447

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSL
CCEEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
ARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLICGHSDFTAIQCGLLAHGN
HHHCCCCEEEEEEECCCCHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCEEEEEEEECCC
VITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQGEGPTCQTEGTLWGGNLAML
EEEEECCEEEEECCCHHHHHHHCCEEEEEEECCEEEEEECCCCCCCCCCCCEECCCHHHH
ISLIGTPWMPQIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGNTPND
HHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
YDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAQAMLNNTQEGTQLTISGHP
CCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCHHHHCCCCCCCEEEECCCC
VLKM
EECC
>Mature Secondary Structure 
SLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCERFAGTETERLEDLNSL
CEEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
ARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLICGHSDFTAIQCGLLAHGN
HHHCCCCEEEEEEECCCCHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCEEEEEEEECCC
VITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQGEGPTCQTEGTLWGGNLAML
EEEEECCEEEEECCCHHHHHHHCCEEEEEEECCEEEEEECCCCCCCCCCCCEECCCHHHH
ISLIGTPWMPQIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGNTPND
HHHHCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
YDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAQAMLNNTQEGTQLTISGHP
CCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEECCCCHHHHCCCCCCCEEEECCCC
VLKM
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]