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The map label for this gene is ynfK

Identifier: 218699841

GI number: 218699841

Start: 1541286

End: 1541981

Strand: Direct

Name: ynfK

Synonym: ECIAI39_1465

Alternate gene names: 218699841

Gene position: 1541286-1541981 (Clockwise)

Preceding gene: 218699840

Following gene: 218699849

Centisome position: 30.03

GC content: 52.01

Gene sequence:

>696_bases
ATGCTGAAGCGTTTCTTTATTACCGGTACAGACACTTCTGTAGGGAAAACGGTGGTTTCCCGCGCATTGCTACAAGCGTT
AGCCTCCCAGGGAAAAACGGTTGCGGGATATAAACCCGTAGCGAAGGGGAGCAAAGAGACACCCGAAGGGCTGCGTAATA
AAGATGCCCTGGTGTTGCAGAGTGTTTCAACCATCGAACTGCCTTATGAAGCAGTTAATCCTATCGCGTTAAGCGAAGAA
GAAAGTAGCGTGGCGCACAGTTGCCCAATCAATTACACCCTCATTTCAAACGGCCTGGCAAACCTGACCGATAAAGTCGA
TCATGTCGTGGTAGAAGGGACTGGCGGCTGGCGCAGTCTGATGAATGATTTGCGTCCGCTCTCTGAATGGGTGGTGCAGG
AACAACTGCCGGTGTTGATGGTTGTCGGTATTCAGGAAGGTTGCATTAACCATGCACTGCTAACAGCTCAGGCGATTGCC
AACGACGGGCTGCCGCTCATTGGCTGGGTGGCTAACCGAATCAACCCAGGACTTGCGCATTATGCGGAAATCATTGATGT
GCTCGGTAAAAAACTTCCGGCACCGCTCATTGGTGAACTGCCTTATCTGCCGCGCGCCGAACAGCGTGAACTGGGGCAAT
ACATCCGCTTAGCTATGCTTCGCAGTGTGCTGGCGGTAGATAGAGTCACGGTGTAA

Upstream 100 bases:

>100_bases
TTTGCGCTATCTCAATTTGGGCCAGGAAAGCATAACTTAGACTTTCAAGGTTAATTATTTTCCTGGTTTATATTTGTGAA
GCATAACGGTGGAGTTAGTG

Downstream 100 bases:

>100_bases
CGTCCGCGAAATTACCGACGCAATTACGCAGGCAATCAATAAACCGGGGAGTAGCTGATACTCCCCGGTCATTTCACATA
TCATCAACGTCGACATAATC

Product: putative dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGSKETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEE
ESSVAHSCPINYTLISNGLANLTDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLAMLRSVLAVDRVTV

Sequences:

>Translated_231_residues
MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGSKETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEE
ESSVAHSCPINYTLISNGLANLTDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLAMLRSVLAVDRVTV
>Mature_231_residues
MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGSKETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEE
ESSVAHSCPINYTLISNGLANLTDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLAMLRSVLAVDRVTV

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081944, Length=231, Percent_Identity=99.5670995670996, Blast_Score=466, Evalue=1e-133,
Organism=Escherichia coli, GI1786995, Length=225, Percent_Identity=51.1111111111111, Blast_Score=223, Evalue=9e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 24967; Mature: 24967

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGSKETPEGLRNKDALVLQ
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHCCCCHHHHH
SVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANLTDKVDHVVVEGTGGWRSL
HHHHHCCCHHHCCCCEECCCHHHHHHCCCCCEEHHHCCHHHHHHHHCEEEEECCCCHHHH
MNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIANDGLPLIGWVANRINPGLAH
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
YAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLAMLRSVLAVDRVTV
HHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGSKETPEGLRNKDALVLQ
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHCCCCHHHHH
SVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANLTDKVDHVVVEGTGGWRSL
HHHHHCCCHHHCCCCEECCCHHHHHHCCCCCEEHHHCCHHHHHHHHCEEEEECCCCHHHH
MNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIANDGLPLIGWVANRINPGLAH
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHH
YAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLAMLRSVLAVDRVTV
HHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]