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The map label for this gene is ynfL

Identifier: 218699839

GI number: 218699839

Start: 1538913

End: 1539806

Strand: Direct

Name: ynfL

Synonym: ECIAI39_1463

Alternate gene names: 218699839

Gene position: 1538913-1539806 (Clockwise)

Preceding gene: 218699835

Following gene: 218699840

Centisome position: 29.99

GC content: 52.24

Gene sequence:

>894_bases
ATGAATATTGAACTTCGTCATCTGCGTTACTTTGTTGCGGTTGCAGAAGAGCTGCATTTCGGGCGCGCCGCTGCCCGCCT
GAATATTTCGCAACCACCGCTAAGTCAGCAGATTCAGGCGCTGGAGCAACAAATTGGTGCCCGACTGCTGGCACGAACCA
ATCGCAGTGTATTGCTGACGGCAGCAGGAAAACAGTTTCTTGCAGATAGTCGGCAAATCCTGTCTATGGTGGATGACGCT
GCCGCTCGCGCTGAAAGACTGCATCAGGGTGAAGCGGGGGAGTTGCGTATTGGTTTTACTTCGTCCGCTCCTTTTATTCG
GGCGGTGTCCGATACGTTATCGCTGTTTCGCCGTGATTATCCTGATGTCCATTTACAAACCCGCGAAATGAACACTCGCG
AGCAAATCGCTCCGCTCATCGAAGGAACGCTGGATATGGGATTGCTGCGTAACACAGCGTTACCGGAGACGCTTGAACAC
GCAGTCATCGTCCATGAACCGCTTATGGCGATGATCCCGCACGATCATCCCCTGGCAAATAACCCGAATGTAACGCTGGC
CGAACTGGCGAAAGAACCCTTTGTCTTTTTTGATCCGCACGTCGGGACAGGGCTGTATGACGATATTCTCGGACTGATGC
GACGTTACCATTTGACGCCCGTCATCACTCAGGAGGTGGGTGAGGCAATGACCATCATCGGTCTGGTTTCCGCTGGTCTG
GGCGTTTCAATTTTGCCTGCGTCATTTAAACGTGTTCAGCTCAACGAAATGCGCTGGGTGCCGATTGCTGAAGAGGATGC
GGTTTCTGAAATGTGGTTGGTCTGGCCGAAACATCATGAACAAAGTCCAGCTGCGCGAAACTTTCGTATTCATCTGCTGA
ATGCTCTCAGGTGA

Upstream 100 bases:

>100_bases
TCCTTGCTAAATATGCCTGTGGATCAGGCTTATGGATAGATTAGGAAAATCGAATTGTTCTGTCTAATATATTAATAATC
TCAAATAAGATGTTTTAAAT

Downstream 100 bases:

>100_bases
GGGAAATTTCAGCGAAAAAGCCCGAAAAATGTGCTGTTAATCACATGCCTAAGTAAAAATTTGACGACACGTATTGAAGT
GCTTCACCATAGCCTACAGA

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDA
AARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGL
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR

Sequences:

>Translated_297_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDA
AARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGL
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR
>Mature_297_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDA
AARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGL
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=297, Percent_Identity=99.6632996632997, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1788887, Length=286, Percent_Identity=36.3636363636364, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI2367136, Length=257, Percent_Identity=38.1322957198444, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1790399, Length=248, Percent_Identity=31.8548387096774, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788748, Length=247, Percent_Identity=29.1497975708502, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI145693105, Length=295, Percent_Identity=26.7796610169492, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI157672245, Length=292, Percent_Identity=29.7945205479452, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=32.8205128205128, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788297, Length=252, Percent_Identity=28.1746031746032, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=26.8292682926829, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787530, Length=269, Percent_Identity=26.7657992565056, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786713, Length=258, Percent_Identity=28.2945736434109, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790262, Length=253, Percent_Identity=24.5059288537549, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787806, Length=285, Percent_Identity=23.859649122807, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788481, Length=301, Percent_Identity=25.2491694352159, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787589, Length=94, Percent_Identity=43.6170212765958, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789506, Length=284, Percent_Identity=22.887323943662, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789204, Length=251, Percent_Identity=26.2948207171315, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789173, Length=130, Percent_Identity=32.3076923076923, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=163, Percent_Identity=32.5153374233129, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI87082132, Length=299, Percent_Identity=24.7491638795987, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=26.962457337884, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33296; Mature: 33296

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLT
CCCCHHHHHHHHHHHHHHHHCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
AAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDY
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
PDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLMAMIPHDHPLAN
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCCCCCC
NPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGL
CCCEEHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR
CCEECCCCHHHEECCCEEECCCCCHHHHHHHEEECCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLT
CCCCHHHHHHHHHHHHHHHHCHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
AAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDY
ECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
PDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLMAMIPHDHPLAN
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCCCCCC
NPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGL
CCCEEHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
GVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR
CCEECCCCHHHEECCCEEECCCCCHHHHHHHEEECCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]