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The map label for this gene is fumA

Identifier: 218699822

GI number: 218699822

Start: 1520127

End: 1521773

Strand: Direct

Name: fumA

Synonym: ECIAI39_1446

Alternate gene names: 218699822

Gene position: 1520127-1521773 (Clockwise)

Preceding gene: 218699819

Following gene: 218699823

Centisome position: 29.62

GC content: 50.58

Gene sequence:

>1647_bases
ATGTCAAACAAACCCTTTCATTATCAGGCTCCTTTTCCACTCAAAAAAGATGATACTGAGTATTACCTGCTAACCAGCGA
ACACGTTAGCGTATCTGAATTTGAAGGGCAGGAGATTTTGAAAGTCGCACCCGAAGCGTTAACTCTGTTGGCGCGTCAAG
CGTTTCATGATGCGTCATTTATGCTGCGTCCGGCTCACCAACAACAGGTGGCCGACATTCTGCGTGACCCGGAAGCCAGC
GAAAATGATAAATATGTGGCGCTGCAATTCCTGCGTAACTCCGACATCGCCGCAAAAGGCGTTCTGCCAACCTGTCAGGA
TACCGGCACCGCGATTATTGTTGGTAAAAAAGGGCAGCGTGTATGGACCGGTGGCGGTGATGAAGCAGCGCTGGCGCGCG
GTGTCTATAACACTTATATCGAGGATAATCTGCGCTACTCGCAAAACGCGCCGCTGGATATGTATAAAGAGGTGAATACC
GGCACTAACTTACCAGCGCAGATCGATCTTTATGCCGTTGATGGTGACGAGTACAAATTCCTCTGTATCGCCAAAGGTGG
CGGTTCGGCAAACAAGACGTATCTGTATCAGGAAACCAAAGCGTTACTGACGCCGGGAAAACTGAAAAATTACCTGGTTG
AGAAAATGCGCACGCTGGGTACGGCAGCCTGTCCTCCCTATCATATTGCATTTGTGATTGGTGGAACTTCTGCGGAAACG
AACCTTAAAACGGTGAAACTGGCTTCCGCTAAATACTATGATGAACTGCCAACGGAAGGGAATGAGCACGGACAGGCGTT
CCGCGATGTGGAACTGGAAAAAGAATTGCTGATCGAAGCGCAAAATCTTGGTCTGGGTGCGCAGTTTGGTGGTAAATACT
TCGCTCACGACATCCGCGTGATTCGCCTGCCACGTCACGGCGCATCCTGCCCGGTTGGAATGGGCGTCTCCTGTTCTGCT
GACCGTAATATCAAAGCGAAGATCAACCGTCAGGGGATCTGGATCGAAAAACTGGAACATAATCCAGGCAAATATATCCC
GGAAGAGCTGCGCAAAGCGGGAGAAGGCGAAGCGGTGCGCGTTGACCTTAACCGTCCGATGAAAGAGATCCTCGCACAGT
TGTCGCAGTATCCCGTTTCTACACGCTTATCGCTGAATGGCACGATTATCGTCGGTCGTGATATTGCTCACGCCAAACTG
AAAGAGCGGATGGATAACGGTGAAGGGCTGCCGCAGTACATCAAAGATCATCCGATTTACTATGCGGGTCCGGCCAAAAC
GCCGGAAGGTTATGCCTCCGGTTCTCTTGGCCCAACGACCGCCGGACGGATGGATTCTTATGTCGATCAACTGCAAGCGC
AGGGAGGAAGTATGATCATGCTGGCGAAAGGCAACCGCAGCCAGCAGGTGACGGATGCCTGTAAAAAACACGGCGGTTTC
TACCTCGGTAGTATCGGTGGTCCGGCAGCAGTATTGGCGCAGGGAAGTATTAAGAGCCTGGAATGTGTTGAATATCCGGA
ACTGGGAATGGAAGCCATCTGGAAAATTGAAGTGGAAGATTTCCCGGCGTTTATCCTTGTGGATGATAAAGGAAATGACT
TCTTCCAGCAGATACAACTCACACAATGCACCCGCTGTGTGAAATAA

Upstream 100 bases:

>100_bases
TTTTGTTAAAAAAGTGTGTAGGATATTGTTACTCGCTTTTAACAGGGCAACGGAACACCCGCCCAGAGCATAACCAAACC
AGGCAGTAAGTGAGAGAACA

Downstream 100 bases:

>100_bases
ACAGAGCCGCCCTTCGGGGCGGTTTTTTTACATGGCACGAAAGACCAAACATTTGTTATCAAATAGTAAATAATGAGTGA
GCTAAAAGTTGCTTAACGAA

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPEAS
ENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAET
NLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKL
KERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGF
YLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK

Sequences:

>Translated_548_residues
MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPEAS
ENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAET
NLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSA
DRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKL
KERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGF
YLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK
>Mature_547_residues
SNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAPEALTLLARQAFHDASFMLRPAHQQQVADILRDPEASE
NDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTG
TNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAETN
LKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSAD
RNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLK
ERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGFY
LGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=548, Percent_Identity=100, Blast_Score=1138, Evalue=0.0,
Organism=Escherichia coli, GI1790564, Length=548, Percent_Identity=89.963503649635, Blast_Score=1028, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=181, Percent_Identity=28.7292817679558, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 60299; Mature: 60168

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAPEALTLLARQAFHDASF
CCCCCEEEECCCCCCCCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCHH
MLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQR
EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCE
VWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKF
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEECCCCEEE
LCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAET
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRV
CEEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEHHCCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVR
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHCCCCCEEE
VDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIY
EECCCCHHHHHHHHHHCCCEEEEEECEEEEEECCHHHHHHHHHHCCCCCCHHHHHCCCEE
YAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGF
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQL
EEECCCCCEEEEECCCCCCEEECCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
TQCTRCVK
HHHHHHCC
>Mature Secondary Structure 
SNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAPEALTLLARQAFHDASF
CCCCEEEECCCCCCCCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCHH
MLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVLPTCQDTGTAIIVGKKGQR
EECCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCCEEEEECCCCE
VWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNTGTNLPAQIDLYAVDGDEYKF
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCEEEEEEECCCCEEE
LCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRTLGTAACPPYHIAFVIGGTSAET
EEEECCCCCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKELLIEAQNLGLGAQFGGKYFAHDIRV
CEEEEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEHHCCCCCCCCCCCEEEECEEE
IRLPRHGASCPVGMGVSCSADRNIKAKINRQGIWIEKLEHNPGKYIPEELRKAGEGEAVR
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHCCCCCEEE
VDLNRPMKEILAQLSQYPVSTRLSLNGTIIVGRDIAHAKLKERMDNGEGLPQYIKDHPIY
EECCCCHHHHHHHHHHCCCEEEEEECEEEEEECCHHHHHHHHHHCCCCCCHHHHHCCCEE
YAGPAKTPEGYASGSLGPTTAGRMDSYVDQLQAQGGSMIMLAKGNRSQQVTDACKKHGGF
EECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCE
YLGSIGGPAAVLAQGSIKSLECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFQQIQL
EEECCCCCEEEEECCCCCCEEECCCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHHHH
TQCTRCVK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]