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The map label for this gene is tyrS

Identifier: 218699794

GI number: 218699794

Start: 1490022

End: 1491296

Strand: Direct

Name: tyrS

Synonym: ECIAI39_1418

Alternate gene names: 218699794

Gene position: 1490022-1491296 (Clockwise)

Preceding gene: 218699793

Following gene: 218699795

Centisome position: 29.03

GC content: 51.53

Gene sequence:

>1275_bases
ATGGCAAGCAGTAACTTGATTAAACAATTGCAAGAGCGGGGGCTGGTAGCCCAGGTGACGGACGAGGAAGCGTTAGCAGA
GCGACTGGCGCAAGGCCCGATCGCGCTCTATTGCGGCTTCGATCCTACCGCTGACAGCTTGCATTTGGGGCATCTTGTTC
CATTGTTATGCCTGAAACGCTTCCAGCAGGCGGGCCACAAGCCGGTTGCGCTGGTAGGCGGCGCGACGGGTCTGATTGGC
GACCCGAGCTTCAAAGCTGCTGAGCGTAAGCTGAACACCGAAGAAACTGTTCAGGAATGGGTGGACAAAATCCGCAAGCA
GGTTGCCCCGTTCCTCGATTTCGACTGTGGAGAAAACTCTGCTATCGCGGCGAACAACTATGACTGGTTCGGCAATATGA
ATGTGCTGACCTTCCTGCGCGATATTGGCAAACACTTCTCCGTTAACCAGATGATCAACAAAGAAGCGGTTAAGCAGCGT
CTCAACCGTGAAGATCAGGGGATTTCGTTCACTGAGTTTTCCTACAACCTGTTGCAGGGTTATGACTTCGCCTGTCTGAA
CAAACAGTACGGTGTGGTGCTGCAAATTGGTGGTTCTGACCAGTGGGGTAATATCACTTCTGGTATCGACCTGACCCGTC
GTCTGCATCAGAATCAGGTGTTTGGCCTGACCGTTCCGCTGATCACCAAAGCAGACGGCACCAAATTTGGTAAAACTGAA
GGCGGCGCAGTCTGGTTGGATCCGAAGAAAACCAGCCCGTACAAATTCTACCAGTTCTGGATTAACACTGCGGATGCCGA
CGTTTACCGCTTCCTGAAGTTCTTCACCTTTATGAGCATTGAAGAGATCAACGCCCTGGAAGAAGAAGATAAAAACAGCG
GTAAAGCACCGCGTGCCCAGTATGTACTGGCGGAGCAGGTGACTCGTCTGGTTCACGGTGAAGAAGGTTTACAGGCGGCA
AAACGTATTACCGAATGCCTGTTCAGCGGTTCTTTGAGTGCGCTGAGTGAAGCGGACTTCGAACAGCTGGCGCAGGACGG
CGTACCGATGGTTGAGATGGAAAAGGGCGCAGACCTGATGCAGGCGCTGGTCGATTCTGAACTGCAACCTTCCCGTGGTC
AGGCGCGTAAAACTATCGCCTCTAATGCCATCACCATTAACGGTGAAAAACAGTCCGATCCTGAATACTTCTTTAAAGAA
GAAGATCGTCTGTTTGGTCGTTTTACCTTACTGCGTCGCGGCAAAAAGAATTACTGTCTGATTTGCTGGAAATAA

Upstream 100 bases:

>100_bases
TGGTACTCCTGATTCTGGCACTTTATTCTATGTCTCTTTCGCATCTGGCGAAAAGTCGTGTACCGGCAAAGGTGCAGTCG
TTATATACATGGAGATTTTG

Downstream 100 bases:

>100_bases
TGCATTAAGTGGAAAGGGGGAGTGAGAAATCACTCCCCCTGGTTTTTATACAGGGAACATGATGAAAAATATTCTCGCTA
TCCAGTCTCACGTTGTTTAT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIG
DPSFKAAERKLNTEETVQEWVDKIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTE
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAA
KRITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQSDPEYFFKE
EDRLFGRFTLLRRGKKNYCLICWK

Sequences:

>Translated_424_residues
MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIG
DPSFKAAERKLNTEETVQEWVDKIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTE
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAA
KRITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQSDPEYFFKE
EDRLFGRFTLLRRGKKNYCLICWK
>Mature_423_residues
ASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGD
PSFKAAERKLNTEETVQEWVDKIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQRL
NREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTEG
GAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAK
RITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQSDPEYFFKEE
DRLFGRFTLLRRGKKNYCLICWK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=407, Percent_Identity=37.3464373464373, Blast_Score=254, Evalue=9e-68,
Organism=Escherichia coli, GI1787925, Length=424, Percent_Identity=100, Blast_Score=880, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25145802, Length=410, Percent_Identity=35.8536585365854, Blast_Score=234, Evalue=9e-62,
Organism=Caenorhabditis elegans, GI25145805, Length=333, Percent_Identity=33.3333333333333, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6325160, Length=438, Percent_Identity=35.6164383561644, Blast_Score=271, Evalue=2e-73,
Organism=Drosophila melanogaster, GI20130383, Length=447, Percent_Identity=36.9127516778523, Blast_Score=299, Evalue=2e-81,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47527; Mature: 47396

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKR
CCCHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
FQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVDKIRKQVAPFLDFDCGENS
HHHCCCCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
AIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQRLNREDQGISFTEFSYNLLQG
EEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHC
YDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTE
CCEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCC
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSIEEINALEEEDKNSGKAPRAQ
CCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
YVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLM
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHH
QALVDSELQPSRGQARKTIASNAITINGEKQSDPEYFFKEEDRLFGRFTLLRRGKKNYCL
HHHHCCCCCCCCCHHHHHHHCCEEEECCCCCCCCCHHHCCHHHHHHHHHHHHCCCCCEEE
ICWK
EEEC
>Mature Secondary Structure 
ASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTADSLHLGHLVPLLCLKR
CCHHHHHHHHHCCCEEEECCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHH
FQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVDKIRKQVAPFLDFDCGENS
HHHCCCCCEEEECCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
AIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQRLNREDQGISFTEFSYNLLQG
EEEECCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHC
YDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTE
CCEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCCC
GGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSIEEINALEEEDKNSGKAPRAQ
CCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
YVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLM
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHH
QALVDSELQPSRGQARKTIASNAITINGEKQSDPEYFFKEEDRLFGRFTLLRRGKKNYCL
HHHHCCCCCCCCCHHHHHHHCCEEEECCCCCCCCCHHHCCHHHHHHHHHHHHCCCCCEEE
ICWK
EEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA