BacMap

Definition	Escherichia coli IAI39 chromosome, complete genome.
Accession	NC_011750
Length	5,132,068

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The map label for this gene is gra-orf5 [H]

Identifier: 218699734

GI number: 218699734

Start: 1421812

End: 1422555

Strand: Direct

Name: gra-orf5 [H]

Synonym: ECIAI39_1355

Alternate gene names: 218699734

Gene position: 1421812-1422555 (Clockwise)

Preceding gene: 218699733

Following gene: 218699740

Centisome position: 27.7

GC content: 46.51

Gene sequence:

>744_bases
ATGACAAAAATTGCCCTGGTGACGGGGGGAAGTAAAGGCATCGGCTACGCAGCAGCAAAGGCACTCTATGACATTGGATA
TACAGTTATTGTTGCCGCGCGTAACGAAGAAACCTTACAGCAATGTGCACAAGGATTAGCCAAGGAACGCTTTATTCCTC
TGGTTGCCGATGTCACGGATGAGTCCGCTGTCGTTGCGCTATTTGCCGAGATTAAGGCTCTTTTTGGTCGACTGGATCTC
TTGTTCAATAATGCGGGCAATAATAGTCCGGCAAAAAGTATTGAAGAGGTTGAGTTTACTGAGTGGACGCGCGTGTTTAA
CGCCAATATTCATTCGACATTTTTGTGTTCGAAAGAGGCGATTAAAATCATGAAAGCGCAGCAGCCAATGGGAGGACGTA
TTATTAACAATGCGTCGATTTCTGCACTGACGCCGCGCCTCTATACTGCGCCATACACGGCGTCGAAACATGCTGTTACC
GGGTTGACCAAGGCCATTTCCCTGGATTGTCGCAAATTTAATATCGCTTGCGGGCAAATTAATATCGGCAATGCGGAAAC
GACATTATCGAATCGGATGCGTAAGGGCGTTTATCAGGCAGATGGTGCTATTAAAGCGGAAGATATGATGAATGTTGCTG
ATGTTGCAGCGGCCATTCAGATGATGGCTCAGTTGCCAGTAACCACGAATGTGCTGGAAATAACGATTATGGCAAATAAT
ATGCCGTATGTCGGACGTGGTTAA

Upstream 100 bases:

>100_bases
GGAAGAAGTGCATCGGGCATGCTATAAAATCGATAACGTGGAAAAAATCAAAAAAACAGTCGTGAAAATCGACATGAGTT
TATTATAGGCGGAGCTGGCT

Downstream 100 bases:

>100_bases
TCGAGATTGAATAAAGCACCTTGCGTGTAAGGTGCTTTATTATTTATGACTTATGCCTCAACGCATGTTTGTTCCAGCCG
TTGAAGAATATCTTTACGTA

Product: putative short-chain dehydrogenase/reductase

Products: NA

Alternate protein names: ORF5 [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MTKIALVTGGSKGIGYAAAKALYDIGYTVIVAARNEETLQQCAQGLAKERFIPLVADVTDESAVVALFAEIKALFGRLDL
LFNNAGNNSPAKSIEEVEFTEWTRVFNANIHSTFLCSKEAIKIMKAQQPMGGRIINNASISALTPRLYTAPYTASKHAVT
GLTKAISLDCRKFNIACGQINIGNAETTLSNRMRKGVYQADGAIKAEDMMNVADVAAAIQMMAQLPVTTNVLEITIMANN
MPYVGRG

Sequences:

>Translated_247_residues
MTKIALVTGGSKGIGYAAAKALYDIGYTVIVAARNEETLQQCAQGLAKERFIPLVADVTDESAVVALFAEIKALFGRLDL
LFNNAGNNSPAKSIEEVEFTEWTRVFNANIHSTFLCSKEAIKIMKAQQPMGGRIINNASISALTPRLYTAPYTASKHAVT
GLTKAISLDCRKFNIACGQINIGNAETTLSNRMRKGVYQADGAIKAEDMMNVADVAAAIQMMAQLPVTTNVLEITIMANN
MPYVGRG
>Mature_246_residues
TKIALVTGGSKGIGYAAAKALYDIGYTVIVAARNEETLQQCAQGLAKERFIPLVADVTDESAVVALFAEIKALFGRLDLL
FNNAGNNSPAKSIEEVEFTEWTRVFNANIHSTFLCSKEAIKIMKAQQPMGGRIINNASISALTPRLYTAPYTASKHAVTG
LTKAISLDCRKFNIACGQINIGNAETTLSNRMRKGVYQADGAIKAEDMMNVADVAAAIQMMAQLPVTTNVLEITIMANNM
PYVGRG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI109715829, Length=243, Percent_Identity=29.2181069958848, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI59889578, Length=174, Percent_Identity=30.4597701149425, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI224922801, Length=177, Percent_Identity=34.4632768361582, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI31542939, Length=172, Percent_Identity=34.3023255813954, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI32483357, Length=196, Percent_Identity=29.0816326530612, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI40254992, Length=195, Percent_Identity=28.7179487179487, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI209862901, Length=197, Percent_Identity=28.9340101522843, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI19923983, Length=197, Percent_Identity=28.9340101522843, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI126723191, Length=187, Percent_Identity=28.8770053475936, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI19923817, Length=219, Percent_Identity=28.310502283105, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI4758504, Length=182, Percent_Identity=30.2197802197802, Blast_Score=65, Evalue=8e-11,
Organism=Homo sapiens, GI4503817, Length=177, Percent_Identity=28.2485875706215, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1788459, Length=180, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1789208, Length=196, Percent_Identity=34.1836734693878, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI2367175, Length=174, Percent_Identity=37.9310344827586, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787335, Length=202, Percent_Identity=31.6831683168317, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1789057, Length=188, Percent_Identity=29.2553191489362, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082160, Length=195, Percent_Identity=31.7948717948718, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1790717, Length=192, Percent_Identity=28.125, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786812, Length=203, Percent_Identity=28.0788177339901, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789378, Length=179, Percent_Identity=30.1675977653631, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787526, Length=194, Percent_Identity=28.8659793814433, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787820, Length=250, Percent_Identity=24.4, Blast_Score=61, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17561402, Length=205, Percent_Identity=31.219512195122, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17560676, Length=190, Percent_Identity=28.9473684210526, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17559104, Length=208, Percent_Identity=29.8076923076923, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17538182, Length=198, Percent_Identity=30.8080808080808, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=201, Percent_Identity=28.8557213930348, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=217, Percent_Identity=27.1889400921659, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562906, Length=201, Percent_Identity=27.8606965174129, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=204, Percent_Identity=27.4509803921569, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193204405, Length=204, Percent_Identity=28.4313725490196, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17560150, Length=202, Percent_Identity=28.7128712871287, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538480, Length=190, Percent_Identity=28.9473684210526, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322227, Length=165, Percent_Identity=32.1212121212121, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322067, Length=193, Percent_Identity=26.9430051813472, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI116007236, Length=255, Percent_Identity=28.2352941176471, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24641388, Length=246, Percent_Identity=29.6747967479675, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21355319, Length=169, Percent_Identity=30.1775147928994, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17737361, Length=176, Percent_Identity=35.2272727272727, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI28571526, Length=197, Percent_Identity=30.9644670050761, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI116007238, Length=183, Percent_Identity=29.5081967213115, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI116007234, Length=256, Percent_Identity=27.34375, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281364494, Length=247, Percent_Identity=27.1255060728745, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI23397609, Length=230, Percent_Identity=28.2608695652174, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24641232, Length=172, Percent_Identity=31.9767441860465, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21357041, Length=197, Percent_Identity=29.4416243654822, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI116007232, Length=184, Percent_Identity=29.3478260869565, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24639444, Length=187, Percent_Identity=29.9465240641711, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24580925, Length=182, Percent_Identity=27.4725274725275, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24644339, Length=238, Percent_Identity=27.3109243697479, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI28571387, Length=257, Percent_Identity=29.1828793774319, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648944, Length=200, Percent_Identity=29.5, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24663911, Length=245, Percent_Identity=26.9387755102041, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648693, Length=245, Percent_Identity=24.8979591836735, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21355889, Length=245, Percent_Identity=24.8979591836735, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24643142, Length=179, Percent_Identity=31.8435754189944, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26557; Mature: 26426

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIALVTGGSKGIGYAAAKALYDIGYTVIVAARNEETLQQCAQGLAKERFIPLVADVTD
CCEEEEEECCCCCCHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHCCCEEEEECCC
ESAVVALFAEIKALFGRLDLLFNNAGNNSPAKSIEEVEFTEWTRVFNANIHSTFLCSKEA
CHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECHHH
IKIMKAQQPMGGRIINNASISALTPRLYTAPYTASKHAVTGLTKAISLDCRKFNIACGQI
HHHHHHCCCCCCEEECCCCCCEECCEEEECCCCCCCHHHHHHHHHHEECCEEEEEEECEE
NIGNAETTLSNRMRKGVYQADGAIKAEDMMNVADVAAAIQMMAQLPVTTNVLEITIMANN
ECCCCHHHHHHHHHHCHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
MPYVGRG
CCCCCCC
>Mature Secondary Structure 
TKIALVTGGSKGIGYAAAKALYDIGYTVIVAARNEETLQQCAQGLAKERFIPLVADVTD
CEEEEEECCCCCCHHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHCCCEEEEECCC
ESAVVALFAEIKALFGRLDLLFNNAGNNSPAKSIEEVEFTEWTRVFNANIHSTFLCSKEA
CHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECHHH
IKIMKAQQPMGGRIINNASISALTPRLYTAPYTASKHAVTGLTKAISLDCRKFNIACGQI
HHHHHHCCCCCCEEECCCCCCEECCEEEECCCCCCCHHHHHHHHHHEECCEEEEEEECEE
NIGNAETTLSNRMRKGVYQADGAIKAEDMMNVADVAAAIQMMAQLPVTTNVLEITIMANN
ECCCCHHHHHHHHHHCHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECC
MPYVGRG
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2583128; 7476861; 9831526 [H]