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The map label for this gene is chbF

Identifier: 218699699

GI number: 218699699

Start: 1385022

End: 1386374

Strand: Direct

Name: chbF

Synonym: ECIAI39_1320

Alternate gene names: 218699699

Gene position: 1385022-1386374 (Clockwise)

Preceding gene: 218699698

Following gene: 218699700

Centisome position: 26.99

GC content: 46.49

Gene sequence:

>1353_bases
ATGAGCCAGAAATTAAAAGTCGTCACTATTGGTGGCGGGAGCAGCTATACCCCGGAGTTACTGGAAGGATTTATTAAGCG
TTATCACGAATTGCCGGTCAGCGAATTATGGCTGGTGGATGTCGAAGATGGTAAAGAGAAACTGGATATTATTTTTGATC
TCTGCCAACGGATGATTGATAACGCTGGCGTCCCGATGAAGCTTTATAAAACGCTGGATCGCCGCGAAGCATTGAAAGAT
GCTGATTTCGTTACTACCCAACTGCGCGTAGGCCAATTACCGGCGCGCGAACTGGATGAACGTATTCCATTAAGTCATGG
TTATCTTGGTCAGGAAACCAACGGCGCGGGCGGCCTGTTTAAAGGTCTGCGTACCATTCCGGTGATTTTTGACATCGTAA
AAGATGTCGAAGAACTTTGTCCGAATGCATGGGTGATTAATTTCACTAACCCGGCGGGAATGGTCACTGAAGCCGTTTAT
CGTCATACTGGATTTAAACGCTTTATCGGCGTGTGTAATATTCCGATCGGCATGAAGATGTTTATTCGCGATGTTCTGAT
GCTGAAAGACAGCGATGATTTATCTATCGATCTGTTCGGCCTCAACCATATGGTGTTCATTAAAGATGTGCTGGTAAATG
GCAAATCACGCTTTGCCGAATTGCTTGATGGTGTGGCATCCGGGCAGTTAAAAGCATCTGGCGTTAAAAATATTTTCGAT
CTGCCATTTAGCGAAGGCTTAATTCGTTCTCTGAATCTGTTGCCGTGTTCTTATTTGCTTTATTACTTCAAGCAAAAAGA
GATGCTGGCTATTGAAATGGGCGAATACTACAAAGGCGGCGCACGAGCGCAGGTAGTACAGAAAGTCGAGAAACAACTTT
TTGAGCTGTATAAAAACCCGGAGTTGAAAGTTAAGCCGAAAGAACTGGAACAGCGCGGTGGGGCTTATTACTCTGATGCT
GCATGCGAAGTGATCAACGCTATCTACAACGACAAGCAAGCTGAACATTACGTTAATATCCCGCATCATGGGCATATTGA
TAATATTCCGGCAGACTGGGCGGTAGAAATGACCTGTACGCTGGGGCGCGATGGCGCGACGCCACATCCGCGCATTACGC
ATTTCGATGATAAAGTGATGGGGCTGATTCACACCATTAAAAGCTTCGAGATTGCTGCCAGCAACGCCGCACTTAGCGGA
GAGTTTAACGATGTGTTACTGGCCCTAAACCTTAGTCCGTTGGTGCATTCCGATCGCGATGCTGAGCTGCTGGCACGCGA
GATGATTCTGGCGCACGAGAAATGGTTGCCAAATTTTGCCGACTGCATCGCAGAGCTTAAAAAAGCACATTAA

Upstream 100 bases:

>100_bases
GTTATACCTGACAATTCACATATCTGTCCTGTTGCTGGATTATTTATGTCCGGGGGGCAGATATGCCAGATATCAGTATT
CTGTACTGAAGGGAGAAATT

Downstream 100 bases:

>100_bases
CCGAGGCTGATTATGGAACGCTTACTGATTGTTAATGCCGATGATTTTGGTTTAAGCAAAGGTCAGAACTACGGCATTAT
CGAGGCCTGTCGCAATGGGA

Product: cryptic phospho-beta-glucosidase, NAD(P)-binding

Products: NA

Alternate protein names: Cellobiose-6-phosphate hydrolase; Phospho-chitobiase [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDGKEKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKD
ADFVTTQLRVGQLPARELDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRFAELLDGVASGQLKASGVKNIFD
LPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDA
ACEVINAIYNDKQAEHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKSFEIAASNAALSG
EFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH

Sequences:

>Translated_450_residues
MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDGKEKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKD
ADFVTTQLRVGQLPARELDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRFAELLDGVASGQLKASGVKNIFD
LPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDA
ACEVINAIYNDKQAEHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKSFEIAASNAALSG
EFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
>Mature_449_residues
SQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDGKEKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDA
DFVTTQLRVGQLPARELDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVYR
HTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRFAELLDGVASGQLKASGVKNIFDL
PFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAA
CEVINAIYNDKQAEHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKSFEIAASNAALSGE
FNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH

Specific function: Hydrolyzes a wide variety of P-beta-glucosides including cellobiose-6P, salicin-6P, arbutin-6P, gentiobiose-6P, methyl- beta-glucoside-6P and p-nitrophenyl-beta-D-glucopyranoside-6P. Is also able to hydrolyze phospho-N,N'-diacetylchitobiose [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=450, Percent_Identity=98.4444444444444, Blast_Score=915, Evalue=0.0,
Organism=Escherichia coli, GI1790560, Length=463, Percent_Identity=25.7019438444924, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 50571; Mature: 50440

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDGKEKLDIIFDLCQRMID
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
NAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERIPLSHGYLGQETNGAGGLF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCHHH
KGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVYRHTGFKRFIGVCNIPIGMKM
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHH
FIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRFAELLDGVASGQLKASGVKNIFD
HHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCEECCCCHHHHC
LPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNP
CCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCC
ELKVKPKELEQRGGAYYSDAACEVINAIYNDKQAEHYVNIPHHGHIDNIPADWAVEMTCT
CCEECHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCEEEEEEE
LGRDGATPHPRITHFDDKVMGLIHTIKSFEIAASNAALSGEFNDVLLALNLSPLVHSDRD
ECCCCCCCCCCEECCHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEECCHHHCCCCC
AELLAREMILAHEKWLPNFADCIAELKKAH
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDGKEKLDIIFDLCQRMID
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
NAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERIPLSHGYLGQETNGAGGLF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCHHH
KGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVYRHTGFKRFIGVCNIPIGMKM
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHCCCCCHHHH
FIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRFAELLDGVASGQLKASGVKNIFD
HHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCEECCCCHHHHC
LPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNP
CCCHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCC
ELKVKPKELEQRGGAYYSDAACEVINAIYNDKQAEHYVNIPHHGHIDNIPADWAVEMTCT
CCEECHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCEEEEEEE
LGRDGATPHPRITHFDDKVMGLIHTIKSFEIAASNAALSGEFNDVLLALNLSPLVHSDRD
ECCCCCCCCCCEECCHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEEEEECCHHHCCCCC
AELLAREMILAHEKWLPNFADCIAELKKAH
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2179047; 9097039; 9278503; 8121401; 10572139; 9405618; 10913117 [H]