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The map label for this gene is yoaE

Identifier: 218699615

GI number: 218699615

Start: 1302156

End: 1303712

Strand: Direct

Name: yoaE

Synonym: ECIAI39_1236

Alternate gene names: 218699615

Gene position: 1302156-1303712 (Clockwise)

Preceding gene: 218699609

Following gene: 218699620

Centisome position: 25.37

GC content: 53.82

Gene sequence:

>1557_bases
ATGGAATTCTTAATGGACCCCTCGATTTGGGCGGGGCTACTCACGCTTGTTGTTCTCGAAATTGTGCTGGGTATCGATAA
CCTGGTCTTCATCGCCATTCTTGCTGACAAACTGCCGCCAAAACAACGCGATAAAGCGCGTTTGCTGGGATTATCACTGG
CGCTGATTATGCGTCTGGGGCTGCTGTCGCTGATTTCATGGATGGTCACGCTGACCAAACCGCTATTTACCGTCATGGAT
TTCTCCTTCTCCGGACGCGACCTGATTATGTTGTTCGGGGGGATATTCTTGCTGTTCAAAGCAACAACCGAACTGCATGA
ACGGCTGGAAAACCGCGATCATGATTCCGGCCACGGTAAAGTCTACGCCAGTTTCTGGGTGGTCGTCACACAGATCGTCA
TCCTTGACGCCGTCTTCTCGTTGGATGCGGTAATTACGGCAGTAGGGATGGTTAACCATCTGCCAGTGATGATGGCGGCG
GTAGTGATTGCGATGGCGGTTATGCTGCTGGCATCGAAACCGCTGACGCGATTTGTTAACCAGCATCCAACGGTGGTGGT
GCTCTGTCTGAGCTTCCTGTTAATGATAGGTCTGAGTCTGGTGGCAGAAGGTTTCGGTTTCCACATTCCGAAAGGTTACC
TGTATGCCGCGATTGGCTTCTCGATCATCATCGAAGTGTTTAACCAAATTGCGCGTCGCAACTTTATTCGTCACCAGTCG
ACTTTGCCGCTGCGAGCGCGTACTGCCGATGCCATCCTGCGTTTGATGGGCGGGAAACGTCAGGCCAATGTCCAGCACGA
TGCCGATAACCCGATGCCGATGCCGATCCCGGAAGGTGCATTTGCCGAAGAAGAACGTTACATGATTAACGGCGTACTGA
CGCTGGCGTCGCGTTCTCTGCGCGGGATCATGACGCCGCGCGGTGAAATAAGCTGGGTTGACGCTAATCTCGGGGTCGAT
GAAATCCGCGAGCAACTGCTCTCTTCACCTCACAGTCTGTTCCCGGTATGTCGCGGTGAACTGGATGAAATCATCGGTAT
CGTACGTGCTAAAGAACTGCTGGTGGCGCTGGAAGAGGGCGTTGATGTGGCGGCGATTGCTTCGGCGTCTCCGGCGATTA
TCGTCCCGGAAACCCTCGATCCGATCAACCTGCTGGGCGTACTGCGTCGTGCTCGCGGGAGCTTTGTTATCGTGACCAAC
GAGTTTGGTGTGGTACAAGGTCTGGTCACGCCGCTGGATGTGCTGGAAGCCATTGCGGGTGAATTCCCGGACGCTGACGA
AACGCCGGAAATCATTACCGACGGTGACGGCTGGCTGGTAAAAGGCGGTACAGATTTGCATGCCTTGCAGCAGGCGCTTG
ATGTTGAGCACCTTGCCGATGACGATGATATCGCGACGGTCGCGGGCCTCGTGATCTCGGCAAATGGTCACATTCCCCGT
GTGGGCGATGTGATTGATGTAGGGCCACTGCATATCACCATCATTGAAGCCAATGATTATCGTGTTGATCTGGTTCGCAT
TGTTAAAGAGCAACCGGCGCACGATGAAGATGAGTAA

Upstream 100 bases:

>100_bases
GCCTTTCTGGCACTTTATGCCGCACCTTTCGTTTGCATTTTGTCGTTACGCCTGCATTATTTCTGGCGTCGAATAGCTAT
TCCTTAAGCAGGAGCTTGTC

Downstream 100 bases:

>100_bases
GTGTTAACGTAACGGCATAACGGGCGTGATATGTCCATTATGCCGGGCGGGCGGCGGTTGGCTGCCCGCCAGCCATTTGG
GAAAATCACGTAGCGGCATC

Product: putative membrane protein fused with conserved domain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRDKARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMD
FSFSGRDLIMLFGGIFLLFKATTELHERLENRDHDSGHGKVYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAIGFSIIIEVFNQIARRNFIRHQS
TLPLRARTADAILRLMGGKRQANVQHDADNPMPMPIPEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVD
EIREQLLSSPHSLFPVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGSFVIVTN
EFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQALDVEHLADDDDIATVAGLVISANGHIPR
VGDVIDVGPLHITIIEANDYRVDLVRIVKEQPAHDEDE

Sequences:

>Translated_518_residues
MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRDKARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMD
FSFSGRDLIMLFGGIFLLFKATTELHERLENRDHDSGHGKVYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAIGFSIIIEVFNQIARRNFIRHQS
TLPLRARTADAILRLMGGKRQANVQHDADNPMPMPIPEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVD
EIREQLLSSPHSLFPVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGSFVIVTN
EFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQALDVEHLADDDDIATVAGLVISANGHIPR
VGDVIDVGPLHITIIEANDYRVDLVRIVKEQPAHDEDE
>Mature_518_residues
MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRDKARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMD
FSFSGRDLIMLFGGIFLLFKATTELHERLENRDHDSGHGKVYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAIGFSIIIEVFNQIARRNFIRHQS
TLPLRARTADAILRLMGGKRQANVQHDADNPMPMPIPEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVD
EIREQLLSSPHSLFPVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGSFVIVTN
EFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQALDVEHLADDDDIATVAGLVISANGHIPR
VGDVIDVGPLHITIIEANDYRVDLVRIVKEQPAHDEDE

Specific function: Unknown

COG id: COG1253

COG function: function code R; Hemolysins and related proteins containing CBS domains

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 CBS domains [H]

Homologues:

Organism=Homo sapiens, GI310128564, Length=213, Percent_Identity=26.7605633802817, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788119, Length=518, Percent_Identity=99.8069498069498, Blast_Score=1030, Evalue=0.0,
Organism=Escherichia coli, GI87082033, Length=520, Percent_Identity=50.1923076923077, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1789197, Length=232, Percent_Identity=48.7068965517241, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1790664, Length=240, Percent_Identity=26.6666666666667, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786879, Length=225, Percent_Identity=31.5555555555556, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI145693175, Length=235, Percent_Identity=29.7872340425532, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016169
- InterPro:   IPR000644
- InterPro:   IPR005496
- InterPro:   IPR005170 [H]

Pfam domain/function: PF00571 CBS; PF03471 CorC_HlyC; PF03741 TerC [H]

EC number: NA

Molecular weight: Translated: 56571; Mature: 56571

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCEEECCCEEEEEEECCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure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CCCEEECCCEEEEEEECCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]