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The map label for this gene is ybjI

Identifier: 218699219

GI number: 218699219

Start: 871019

End: 871834

Strand: Reverse

Name: ybjI

Synonym: ECIAI39_0823

Alternate gene names: 218699219

Gene position: 871834-871019 (Counterclockwise)

Preceding gene: 218699220

Following gene: 218699218

Centisome position: 16.99

GC content: 45.83

Gene sequence:

>816_bases
ATGAGCATTAAATTAATTGCGGTAGACATGGATGGTACTTTCTTAAGCGATCAAAAAACCTATAACCGTGAGCGGTTTAT
GGCTCAGTATCAGCAAATGAAAGCACAAGGCATTCGCTTTGTGGTCGCCAGCGGGAATCAATATTATCAGTTGATCTCTT
TCTTTCCTGAAATTGCTAATGAAATTGCCTTTGTGGCAGAAAACGGCGGCTGGGTAGTGAGCGAAGGCAAAGATGTTTTT
AATGGCGAGCTATCGAAGGATGCGTTTACTACTGTCGTGGAACATTTGCTGACGCGCCCGGAAGTGGAAATTATTGCCTG
CGGAAAAAATAGCGCCTATACCCTCAAAAAATATGACGATGCCATGAAAACGGTGGCGGAAATGTATTATCACCGTCTGG
AATACGTCGATAACTTTGACAACTTAGAAGATATCTTCTTTAAATTTGGCCTTAATCTCTCTGACGAACTGATCCCACAA
GTGCAAAAAGCATTACATGAGGCCATCGGCGATATCATGGTACCGGTCCATACCGGCAACGGCAGCATCGATCTGATTAT
CCCCGGTGTACATAAAGCCAATGGCCTTCGCCAACTGCAGAAATTATGGGGAATAGACGACAGCGAAGTGGTGGTCTTTG
GCGATGGCGGTAACGATATTGAGATGCTGCGTCAGGCAGGCTTTAGTTTTGCAATGGAAAATGCCGGCAGCGCGGTCGTC
GCAGCGGCAAAATACCGGGCAGGCTCCAATAACCGTGAAGGCGTACTGGATGTGATCGATAAAGTGCTTAAACACGAAGC
GCCATTTAAGCAATAA

Upstream 100 bases:

>100_bases
CAATGATGGTTGTACTGGCGCTGGTTATTCTCGCGGCTATTGTCGCGAAAGCCGTCGCCAAACCCGATACCAAAACGCAG
ACGGCGATGGAGAATAGTTG

Downstream 100 bases:

>100_bases
AACGGCAGCACGCCCGGCAACACAATACCTGAATGAAGGCTGCATAATTCGCGGAAATTAACCCGCTGCATTATTTCAGG
CGAAATGATTATGAAAAATT

Product: putative phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVF
NGELSKDAFTTVVEHLLTRPEVEIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVV
AAAKYRAGSNNREGVLDVIDKVLKHEAPFKQ

Sequences:

>Translated_271_residues
MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVF
NGELSKDAFTTVVEHLLTRPEVEIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVV
AAAKYRAGSNNREGVLDVIDKVLKHEAPFKQ
>Mature_270_residues
SIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFN
GELSKDAFTTVVEHLLTRPEVEIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQV
QKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVVA
AAKYRAGSNNREGVLDVIDKVLKHEAPFKQ

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=271, Percent_Identity=98.8929889298893, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1787043, Length=269, Percent_Identity=52.0446096654275, Blast_Score=287, Evalue=6e-79,
Organism=Escherichia coli, GI2367265, Length=271, Percent_Identity=26.1992619926199, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 30250; Mature: 30119

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIAN
CCEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHH
EIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEVEIIACGKNSAYTLKKYDD
HEEEEECCCCEEEECCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHH
AMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQVQKALHEAIGDIMVPVHTGN
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCC
GSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVV
CEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCHHHHHCCCCEEE
AAAKYRAGSNNREGVLDVIDKVLKHEAPFKQ
EEHHHCCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIAN
CEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHH
EIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEVEIIACGKNSAYTLKKYDD
HEEEEECCCCEEEECCCHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCEEHHHHHH
AMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQVQKALHEAIGDIMVPVHTGN
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCC
GSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGNDIEMLRQAGFSFAMENAGSAVV
CEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCHHHHHCCCCEEE
AAAKYRAGSNNREGVLDVIDKVLKHEAPFKQ
EEHHHCCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]