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The map label for this gene is gltT [H]

Identifier: 218699095

GI number: 218699095

Start: 740434

End: 741735

Strand: Reverse

Name: gltT [H]

Synonym: ECIAI39_0698

Alternate gene names: 218699095

Gene position: 741735-740434 (Counterclockwise)

Preceding gene: 218699096

Following gene: 218699094

Centisome position: 14.45

GC content: 48.77

Gene sequence:

>1302_bases
GTGCACCTGATGGAGGAAAAAATGAAGAAAATAAGTTTAACCACGATGATTCTTTTGGCGCTGGTACTTGGAATGATTAT
CGGCGTAGTGCTCAATAACACAGCTTCACCGGAAACCGCAAAACTCTATGCGCAAGAAATATCGATATTCACGACGATTT
TCTTACGACTGATAAAAATGATTATCGCTCCGTTAGTGGTCTCTACCCTGGTGGTAGGTATTGCTAAAATGGGCGATGCC
AAAGCCCTTGGTCGTATTTTTTCTAAAACACTCTTTTTATTTATTTGCGCCTCATTGCTGTCAATTGCCTTAGGCTTGAT
AACGGTAAATTTCTTCATGCCAGGCACAGGAATTAATTTTGTTGCACACGGTGCCGAAACCACCGGAGTGGTCGCGTCAG
AACCCTTTACACTAAAAGTATTTATTTCGCATGCTTTCCCCACCAGCATTGTCGATGCCATGGCACACAATGAAATTTTG
CAAATCGTGGTGTTCTCAATTTTCCTCGGCTGTAGCCTGACGGCGATTGGTGAGAAAGGCAGCGCCATCGTTCACGCCTT
AGATTCGCTGGCACATGCCATGTTAAAGCTCACTGGCTACGTCATGCTCTTCGCTCCCCTGACCGTATTCGCCGCTATTT
CAGCATTGATTGCTGAACGAGGACTGGCAGTTATGGTGAGCGCCGGGATCTTTATGGGTGAATTTTATTTCACCATGTTG
TTACTTTGGGTGCTGCTTATCGGTCTGGCCATCGTTTATGTCGGCCCCTGCATCAGACGCCTGACCCGTGCCCTTTCGGA
ACCCGCCCTGCTGGCATTTACCACATCCAGTTCTGAAGCGGCTTTTCCGGGAACGCTTGAAAAACTGGAGCAATTTGGCG
TTTCCCCCAAAATTGCCAGCTTTGTCTTACCCATTGGCTACTCATTTAATCTCGTTGGATCAATGGCCTACTGCTCCTTC
GCCACAGTTTTCATCGCCCAGGCCTGCAATATCCATTTATCCATCGGTGAGCAAATCACCATGCTGTTGATCCTGATGTT
GACCTCGAAAGGAATGGCTGGCGTACCACGCGCCTCAATGGTGGTTATCGCCACCACGCTCAACCAGTTCAATATTCCGG
AAGCGGGGCTGATCTTGCTGATGGGCGTTGATCCGTTCCTTGATATGGGGCGTTCCGCGACAAACGTCATGAGCAACGCA
ATGGGCGCTGCGATGGTGAGTCGGTGGGAAGGCGAACATTTCGGCGAGGGCTGTCGGGGTAAAGCATTAAAACCCAATGA
ATCGAACGTTGCTCTGCCCTGA

Upstream 100 bases:

>100_bases
ATTGCAGTTCAACACTTCAGCTATTTGTAAGGTTGTAATAGCAGGTAGATGCCTGAATCTCTATTCAGGCATCTACTTCT
CCTGATTCTGATTTATCGCC

Downstream 100 bases:

>100_bases
GGGGGATCTTAAGCAATCAGAAGCCAATGACTACGCATTTTTATAAGCCGGATCATGAAGTTAGACGGCAATTAAGTAAC
ACTAACGTTTAAGGTGAAAA

Product: putative symport protein

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MHLMEEKMKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFTTIFLRLIKMIIAPLVVSTLVVGIAKMGDA
KALGRIFSKTLFLFICASLLSIALGLITVNFFMPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEIL
QIVVFSIFLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVMVSAGIFMGEFYFTML
LLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFPGTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSF
ATVFIAQACNIHLSIGEQITMLLILMLTSKGMAGVPRASMVVIATTLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSNA
MGAAMVSRWEGEHFGEGCRGKALKPNESNVALP

Sequences:

>Translated_433_residues
MHLMEEKMKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFTTIFLRLIKMIIAPLVVSTLVVGIAKMGDA
KALGRIFSKTLFLFICASLLSIALGLITVNFFMPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEIL
QIVVFSIFLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVMVSAGIFMGEFYFTML
LLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFPGTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSF
ATVFIAQACNIHLSIGEQITMLLILMLTSKGMAGVPRASMVVIATTLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSNA
MGAAMVSRWEGEHFGEGCRGKALKPNESNVALP
>Mature_433_residues
MHLMEEKMKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFTTIFLRLIKMIIAPLVVSTLVVGIAKMGDA
KALGRIFSKTLFLFICASLLSIALGLITVNFFMPGTGINFVAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEIL
QIVVFSIFLGCSLTAIGEKGSAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVMVSAGIFMGEFYFTML
LLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFPGTLEKLEQFGVSPKIASFVLPIGYSFNLVGSMAYCSF
ATVFIAQACNIHLSIGEQITMLLILMLTSKGMAGVPRASMVVIATTLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSNA
MGAAMVSRWEGEHFGEGCRGKALKPNESNVALP

Specific function: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=479, Percent_Identity=27.1398747390397, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI21314632, Length=423, Percent_Identity=28.1323877068558, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI4827012, Length=284, Percent_Identity=31.3380281690141, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI66773030, Length=454, Percent_Identity=25.7709251101322, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI40254478, Length=465, Percent_Identity=24.7311827956989, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI194239697, Length=284, Percent_Identity=28.5211267605634, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI262359914, Length=416, Percent_Identity=25.7211538461538, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI5032093, Length=390, Percent_Identity=28.2051282051282, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI223468564, Length=261, Percent_Identity=28.735632183908, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI223468566, Length=261, Percent_Identity=28.735632183908, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI301601644, Length=196, Percent_Identity=30.6122448979592, Blast_Score=83, Evalue=4e-16,
Organism=Escherichia coli, GI1790514, Length=425, Percent_Identity=38.8235294117647, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1789947, Length=372, Percent_Identity=36.2903225806452, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1788024, Length=432, Percent_Identity=25, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17537407, Length=449, Percent_Identity=28.9532293986637, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI71996953, Length=486, Percent_Identity=25.3086419753086, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71983099, Length=476, Percent_Identity=25.8403361344538, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71983106, Length=473, Percent_Identity=25.5813953488372, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI193206505, Length=444, Percent_Identity=26.8018018018018, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI193206654, Length=449, Percent_Identity=24.7216035634744, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17541374, Length=136, Percent_Identity=34.5588235294118, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24583025, Length=458, Percent_Identity=26.2008733624454, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI17137668, Length=458, Percent_Identity=26.2008733624454, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24583023, Length=458, Percent_Identity=26.2008733624454, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI281360481, Length=464, Percent_Identity=26.5086206896552, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281360483, Length=464, Percent_Identity=26.5086206896552, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17137666, Length=464, Percent_Identity=26.5086206896552, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46206; Mature: 46206

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLMEEKMKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFTTIFLRLIKM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITVNFFMPGTGINF
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
VAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSIFLGCSLTAIGEKG
EECCCCCCCEEECCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCC
SAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVMVSAGIFMGEFYFTML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHH
LLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFPGTLEKLEQFGVSPKIAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHCCCCHHHHH
FVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITMLLILMLTSKGMAGVPRASM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHCCCCCCCCCCCE
VVIATTLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSNAMGAAMVSRWEGEHFGEGCRG
EEEEEECCCCCCCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
KALKPNESNVALP
CCCCCCCCCCCCC
>Mature Secondary Structure
MHLMEEKMKKISLTTMILLALVLGMIIGVVLNNTASPETAKLYAQEISIFTTIFLRLIKM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IIAPLVVSTLVVGIAKMGDAKALGRIFSKTLFLFICASLLSIALGLITVNFFMPGTGINF
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
VAHGAETTGVVASEPFTLKVFISHAFPTSIVDAMAHNEILQIVVFSIFLGCSLTAIGEKG
EECCCCCCCEEECCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCC
SAIVHALDSLAHAMLKLTGYVMLFAPLTVFAAISALIAERGLAVMVSAGIFMGEFYFTML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHH
LLWVLLIGLAIVYVGPCIRRLTRALSEPALLAFTTSSSEAAFPGTLEKLEQFGVSPKIAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHCCCCHHHHH
FVLPIGYSFNLVGSMAYCSFATVFIAQACNIHLSIGEQITMLLILMLTSKGMAGVPRASM
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCEEEECHHHHHHHHHHHHHCCCCCCCCCCCE
VVIATTLNQFNIPEAGLILLMGVDPFLDMGRSATNVMSNAMGAAMVSRWEGEHFGEGCRG
EEEEEECCCCCCCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
KALKPNESNVALP
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]