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The map label for this gene is lnt

Identifier: 218699021

GI number: 218699021

Start: 658275

End: 659813

Strand: Reverse

Name: lnt

Synonym: ECIAI39_0624

Alternate gene names: 218699021

Gene position: 659813-658275 (Counterclockwise)

Preceding gene: 218699022

Following gene: 218699020

Centisome position: 12.86

GC content: 55.36

Gene sequence:

>1539_bases
ATGGCTTTTGCCTCATTAATTGAACGCCAGCGCATTCGCCTGCTGCTGGCGTTATTATTCGGTGCCTGCGGAACGCTGGC
CTTCTCTCCTTACGACGTCTGGCCTGCGGCGATTATTTCGCTGATGGGGCTTCAGGCGTTGACCTTTAACCGCCGTCCAC
TCCAGTCTGCCGCTATTGGCTTTTGCTGGGGATTTGGCCTCTTTGGCAGCGGTATTAACTGGGTCTATGTCAGCATCGCG
ACCTTTGGCGGAATGCCTGGCCCGGTTAACATATTCCTGGTGGTGCTGCTGGCGGCGTATTTGTCGCTGTATACCGGACT
GTTTGCTGGCGTGCTGTCGCGTCTGTGGCCGAAAACCACCTGGCTGCGCGTGGCGATTGCCGCCCCTGCTCTCTGGCAAG
TGACCGAGTTTCTGCGCGGTTGGGTGCTGACTGGCTTCCCGTGGTTACAGTTCGGCTATAGCCAGATTGATGGCCCGTTA
AAAGGGCTGGCACCGATAATGGGCGTGGAAGCCATTAACTTCCTGCTGATGATGGTTAGTGGCCTGCTGGCACTGGCGTT
GGTCAAACGCAACTGGCGTCCGCTGGTGGTGGCCGTCGTGCTGTTTGCCCTTCCCTTCCCGCTGCGTTACATCCAGTGGT
TTACCCCGCAACCGGAGAAAACCATTCAGGTTTCGATGGTTCAGGGCGATATTCCGCAATCGCTGAAATGGGACGAAGGC
CAGCTTCTTAATACGCTGAAGATTTACTACAACGCAACGGCACCGCTGATGGGCAAATCATCGTTGATTATCTGGCCGGA
GTCGGCGATAACCGATCTGGAAATTAATCAGCAACCGTTCCTCAAAGCTCTGGACGGTGAGTTGCGCGATAAAGGTAGCT
CGCTGGTCACCGGGATTGTCGACGCGCGTCTCAATAAGCAGAACCGCTACGATACCTACAACACCATCATCACACTGGGT
AAAGGCGCACCGTACAGCTATGAATCAGCCGATCGCTATAACAAAAACCATCTGGTGCCGTTTGGCGAGTTTGTCCCGCT
GGAGTCGATTCTGCGTCCGTTAGCACCGTTCTTTGATCTGCCGATGTCGTCGTTCAGCCGTGGGCCATATATCCAGCCGC
CGCTGTCGGTAAATGGTATTGAGCTTACTGCGGCTATTTGCTACGAGATCATTCTCGGCGAGCAAGTGCGCGATAACTTC
CGCCCGGATACCGACTATCTGCTGACTATCTCCAACGATGCGTGGTTTGGTAAGTCTATTGGTCCATGGCAGCACTTCCA
GATGGCGCGAATGCGTGCGTTGGAGCTGGCGCGCCCATTGCTGCGTAGCACCAACAACGGTATTACGGCGGTGATTGGCC
CGCAGGGTGAGATTCAGGCGATGATCCCGCAGTTCACCCGCGAGGTGTTAACCACTAATGTGACACCGACCACCGGACTC
ACACCGTATGCACGTACCGGCAACTGGCCGCTGTGGGTACTGACGGCATTGTTTGGTTTTGCCGCTGTGTTGATGAGTCT
GCGTCAACGACGTAAATAA

Upstream 100 bases:

>100_bases
GGCCGACAGTCGGCGTATTATTCAGGTTCATGTCAAAATCCCGGATGACTCACCCCAGCCGAAGCTGGATGAATAAAACC
GAAACTGGATAGATAACTAC

Downstream 100 bases:

>100_bases
TCCCTTCTTATCGTGCCGGAATACATCCCGTATTTCGGCACGCTCGCCGGATGTGGCATCAGTGCGTTATCCGGATGACA
AAATTCCCCTATCCAACTTT

Product: apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: ALP N-acyltransferase; Copper homeostasis protein CutE [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGLQALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIA
TFGGMPGPVNIFLVVLLAAYLSLYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQPEKTIQVSMVQGDIPQSLKWDEG
QLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEINQQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLG
KGAPYSYESADRYNKNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIILGEQVRDNF
RPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITAVIGPQGEIQAMIPQFTREVLTTNVTPTTGL
TPYARTGNWPLWVLTALFGFAAVLMSLRQRRK

Sequences:

>Translated_512_residues
MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGLQALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIA
TFGGMPGPVNIFLVVLLAAYLSLYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQPEKTIQVSMVQGDIPQSLKWDEG
QLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEINQQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLG
KGAPYSYESADRYNKNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIILGEQVRDNF
RPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITAVIGPQGEIQAMIPQFTREVLTTNVTPTTGL
TPYARTGNWPLWVLTALFGFAAVLMSLRQRRK
>Mature_511_residues
AFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGLQALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIAT
FGGMPGPVNIFLVVLLAAYLSLYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPLK
GLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQPEKTIQVSMVQGDIPQSLKWDEGQ
LLNTLKIYYNATAPLMGKSSLIIWPESAITDLEINQQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGK
GAPYSYESADRYNKNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIILGEQVRDNFR
PDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITAVIGPQGEIQAMIPQFTREVLTTNVTPTTGLT
PYARTGNWPLWVLTALFGFAAVLMSLRQRRK

Specific function: Transfers the fatty acyl group on membrane lipoproteins [H]

COG id: COG0815

COG function: function code M; Apolipoprotein N-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1786878, Length=512, Percent_Identity=99.8046875, Blast_Score=1031, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004563
- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 57095; Mature: 56963

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGLQALTFNRRPLQSAAIG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLSLYTGLFAGVLSRLWPKTT
HHHHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
WLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPLKGLAPIMGVEAINFLLMMVS
HEEHHHHCCHHHHHHHHHHHHHHCCCHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQPEKTIQVSMVQGDIPQSLKWDEG
HHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCC
QLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEINQQPFLKALDGELRDKGSSLVTGIV
HHHHHEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCCHHHHCCHHHHHHHH
DARLNKQNRYDTYNTIITLGKGAPYSYESADRYNKNHLVPFGEFVPLESILRPLAPFFDL
HHHHCCCCCCCCCHHEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PMSSFSRGPYIQPPLSVNGIELTAAICYEIILGEQVRDNFRPDTDYLLTISNDAWFGKSI
CHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
GPWQHFQMARMRALELARPLLRSTNNGITAVIGPQGEIQAMIPQFTREVLTTNVTPTTGL
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCC
TPYARTGNWPLWVLTALFGFAAVLMSLRQRRK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGLQALTFNRRPLQSAAIG
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLSLYTGLFAGVLSRLWPKTT
HHHHHHHHCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
WLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPLKGLAPIMGVEAINFLLMMVS
HEEHHHHCCHHHHHHHHHHHHHHCCCHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQPEKTIQVSMVQGDIPQSLKWDEG
HHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCC
QLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEINQQPFLKALDGELRDKGSSLVTGIV
HHHHHEEEEEECCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHCCHHHHCCHHHHHHHH
DARLNKQNRYDTYNTIITLGKGAPYSYESADRYNKNHLVPFGEFVPLESILRPLAPFFDL
HHHHCCCCCCCCCHHEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PMSSFSRGPYIQPPLSVNGIELTAAICYEIILGEQVRDNFRPDTDYLLTISNDAWFGKSI
CHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
GPWQHFQMARMRALELARPLLRSTNNGITAVIGPQGEIQAMIPQFTREVLTTNVTPTTGL
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCC
TPYARTGNWPLWVLTALFGFAAVLMSLRQRRK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]